Determination of terahertz permittivity of dehydrated biological samples

A key step in transforming terahertz imaging to a practical medical imaging modality lies in understanding the interactions between terahertz (THz) waves and biological tissues. Most of the models in the literature use the permittivity of liquid water to simulate the THz-tissue interactions, but they often neglect contributions from the biological background such as proteins and lipids because dehydrated biological samples are experimentally difficult to prepare. In this work, we present a method to prepare thin and flat dehydrated samples which can be easily handled and measured in a transmission setup. Our results will provide fundamental parameters for modelling THz-tissue interactions.

[1]  G. Jenkin,et al.  Undecalcified Bone Preparation for Histology, Histomorphometry and Fluorochrome Analysis , 2010, Journal of visualized experiments : JoVE.

[2]  P. Taday,et al.  Simulating the response of terahertz radiation to basal cell carcinoma using ex vivo spectroscopy measurements. , 2005, Journal of biomedical optics.

[3]  E. Pickwell‐MacPherson,et al.  Simulation of Terahertz Pulse Propagation in Biological Systems , 2004 .

[4]  Zexuan Zhu,et al.  Observing the Temperature Dependent Transition of the GP2 Peptide Using Terahertz Spectroscopy , 2012, PloS one.

[5]  Valery V Tuchin,et al.  Diffusion characteristics of ethylene glycol in skeletal muscle , 2015, Journal of biomedical optics.

[6]  B A Telfer,et al.  Tissue water content in rats measured by desiccation. , 1997, Journal of pharmacological and toxicological methods.

[7]  Washington Y. Sanchez,et al.  Multiphoton fluorescence microscopy of the live kidney in health and disease , 2014, Journal of biomedical optics.

[8]  Emma Pickwell-MacPherson,et al.  Effects of formalin fixing on the terahertz properties of biological tissues. , 2009, Journal of biomedical optics.

[9]  S. J. Allen,et al.  Probing the collective vibrational dynamics of a protein in liquid water by terahertz absorption spectroscopy , 2006, Protein science : a publication of the Protein Society.

[10]  E. Pickwell‐MacPherson,et al.  Terahertz pulsed imaging of freshly excised human colonic tissues , 2011, Physics in medicine and biology.

[11]  J. Zeitler,et al.  Accurate determination of optical coefficients from chemical samples using terahertz time-domain spectroscopy and effective medium theory. , 2009, Optics letters.

[12]  Richard Baraniuk,et al.  Material parameter estimation with terahertz time-domain spectroscopy. , 2001, Journal of the Optical Society of America. A, Optics, image science, and vision.

[13]  Christopher G. Rylander,et al.  Effect of Localized Mechanical Indentation on Skin Water Content Evaluated Using OCT , 2011, Int. J. Biomed. Imaging.

[14]  E. Pickwell‐MacPherson,et al.  Terahertz spectroscopy of liver cirrhosis: investigating the origin of contrast , 2010, Physics in medicine and biology.

[15]  V. Wallace,et al.  In vivo study of human skin using pulsed terahertz radiation , 2004, Physics in medicine and biology.

[16]  Shuting Fan,et al.  The potential of terahertz imaging for cancer diagnosis: A review of investigations to date. , 2012, Quantitative imaging in medicine and surgery.

[17]  Joo-Hiuk Son,et al.  Terahertz imaging of excised oral cancer at frozen temperature. , 2013, Biomedical optics express.

[18]  S. C. Rojas-Landeros,et al.  Terahertz imaging for early screening of diabetic foot syndrome: A proof of concept , 2017, Scientific Reports.

[19]  B. Fischer,et al.  Terahertz scattering by granular composite materials: An effective medium theory , 2012 .

[20]  W S Grundfest,et al.  Stratified Media Model for Terahertz Reflectometry of the Skin , 2011, IEEE Sensors Journal.

[21]  D. Abbott,et al.  The impact of hydration changes in fresh bio-tissue on THz spectroscopic measurements , 2008, Physics in medicine and biology.

[22]  Emma Pickwell-MacPherson,et al.  The growth of biomedical terahertz research , 2014 .

[23]  Emma Pickwell-MacPherson,et al.  Gelatin embedding: a novel way to preserve biological samples for terahertz imaging and spectroscopy , 2015, Physics in medicine and biology.

[24]  Valery V. Tuchin,et al.  Optical characterization and composition of abdominal wall muscle from rat , 2009 .

[25]  I. Al-Naib,et al.  Limitation in thin-film sensing with transmission-mode terahertz time-domain spectroscopy. , 2011, Optics express.

[26]  W. Grundfest,et al.  THz Medical Imaging: in vivo Hydration Sensing , 2011, IEEE Transactions on Terahertz Science and Technology.

[27]  G R McCahan,et al.  Thermal properties calculated from measured water content as a function of depth in porcine skin. , 1986, Burns, including thermal injury.

[28]  Vincent P Wallace,et al.  Classification of terahertz-pulsed imaging data from excised breast tissue. , 2012, Journal of biomedical optics.

[29]  Emma Pickwell-MacPherson,et al.  Freeze-thaw hysteresis effects in terahertz imaging of biomedical tissues. , 2016, Biomedical optics express.

[30]  C. Joerdens,et al.  Evaluation of leaf water status by means of permittivity at terahertz frequencies , 2009, Journal of biological physics.

[31]  P. Taday,et al.  Terahertz Pulsed Spectroscopy of Human Basal Cell Carcinoma , 2006, Applied spectroscopy.

[32]  Valery V. Tuchin,et al.  The characteristic time of glucose diffusion measured for muscle tissue at optical clearing , 2013 .

[33]  H. Senn,et al.  Terahertz underdamped vibrational motion governs protein-ligand binding in solution , 2014, Nature Communications.

[34]  E. Linfield,et al.  Terahertz pulse imaging of ex vivo basal cell carcinoma. , 2003, The Journal of investigative dermatology.

[35]  H. Looyenga.  Dielectric constants of heterogeneous mixtures , 1965 .