High-resolution mass spectrometry-based selection of peanut peptide biomarkers considering food processing and market type variation.

To protect allergic patients and guarantee correct food labeling, robust, specific and sensitive detection methods are urgently needed. Mass spectrometry (MS)-based methods could overcome the limitations of current detection techniques. The first step in the development of an MS-based method is the identification of biomarkers, which are, in the case of food allergens, peptides. Here, we implemented a strategy to identify the most salient peptide biomarkers in peanuts. Processed peanut matrices were prepared and analyzed using an untargeted approach via high-resolution MS. More than 300 identified peptides were further filtered using selection criteria to strengthen the analytical performance of a future, routine quantitative method. The resulting 16 peptides are robust to food processing, specific to peanuts, and satisfy sequence-based criteria. The aspect of multiple protein isoforms is also considered in the selection tree, an aspect that is essential for a quantitative method's robustness but seldom, if ever, considered.

[1]  Rosa Pilolli,et al.  Chapter 7 - Mass Spectrometry in Food Allergen Research , 2015 .

[2]  Jennifer A. Siepen,et al.  Prediction of missed cleavage sites in tryptic peptides aids protein identification in proteomics. , 2007, Journal of proteome research.

[3]  A. Howard,et al.  Crystal structure of Ara h 3, a major allergen in peanut. , 2009, Molecular immunology.

[4]  Anuradha Balasundaram,et al.  Quantitative Proteomic Profiling of Peanut Allergens in Food Ingredients Used for Oral Food Challenges. , 2016, Analytical chemistry.

[5]  Penelope M. C. Smith,et al.  Analysis of crude protein and allergen abundance in peanuts (Arachis hypogaea cv. Walter) from three growing regions in Australia. , 2013, Journal of agricultural and food chemistry.

[6]  A. Hardacre,et al.  Development of an extruded snack product from the legume Vicia faba minor , 2011 .

[7]  N. Robinson,et al.  Structure-dependent nonenzymatic deamidation of glutaminyl and asparaginyl pentapeptides. , 2004, The journal of peptide research : official journal of the American Peptide Society.

[8]  M. Leippe,et al.  2‐D DIGE analysis of the proteome of extracts from peanut variants reveals striking differences in major allergen contents , 2009, Proteomics.

[9]  Egidio Barbi,et al.  IgE-mediated food allergy in children , 2013, The Lancet.

[10]  Bert Popping,et al.  Allergen detection by mass spectrometry--the new way forward. , 2011, Journal of AOAC International.

[11]  J. Davies,et al.  WHO/IUIS Allergen Nomenclature: Providing a common language , 2018, Molecular immunology.

[12]  P. Arese,et al.  Nutritional value of faba bean (Vicia faba L.) seeds for feed and food , 2010 .

[13]  Vasumathi Dharmavaram,et al.  Multivariate Analysis of the Sequence Dependence of Asparagine Deamidation Rates in Peptides , 2009, Pharmaceutical Research.

[14]  E N C Mills,et al.  The effect of thermal processing on the behaviour of peanut allergen peptide targets used in multiple reaction monitoring mass spectrometry experiments. , 2016, The Analyst.

[15]  Stephen R Quake,et al.  Food allergen detection by mass spectrometry: the role of systems biology , 2016, npj Systems Biology and Applications.

[16]  Arjon J. van Hengel,et al.  Food allergen detection methods and the challenge to protect food-allergic consumers , 2007 .

[17]  Geoffrey J. Barton,et al.  Jalview Version 2—a multiple sequence alignment editor and analysis workbench , 2009, Bioinform..

[18]  A. Huet,et al.  Gluten Analysis in Processed Foodstuffs by a Multi-Allergens and Grain-Specific UHPLC-MS/MS Method: One Method to Detect Them All. , 2019, Journal of AOAC International.

[19]  R. Schwartz,et al.  Peanut allergy: an increasingly common life-threatening disorder. , 2012, Journal of the American Academy of Dermatology.

[20]  V. Spicer,et al.  Effect of cyclization of N-terminal glutamine and carbamidomethyl-cysteine (residues) on the chromatographic behavior of peptides in reversed-phase chromatography. , 2011, Journal of chromatography. A.

[21]  Anthony Trela,et al.  Safety and feasibility of oral immunotherapy to multiple allergens for food allergy , 2014, Allergy, Asthma & Clinical Immunology.

[22]  A. Berger,et al.  On the size of the active site in proteases. I. Papain. , 1967, Biochemical and biophysical research communications.

[23]  Anshu Yang,et al.  Allergen composition analysis and allergenicity assessment of Chinese peanut cultivars. , 2016, Food chemistry.

[24]  A. Sheikh,et al.  Acute and long‐term management of food allergy: systematic review , 2014, Allergy.

[25]  D. Higgins,et al.  Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega , 2011, Molecular systems biology.

[26]  K. Gevaert,et al.  Selection of egg peptide biomarkers in processed food products by high resolution mass spectrometry. , 2019, Journal of chromatography. A.

[27]  N. Robinson,et al.  Prediction of primary structure deamidation rates of asparaginyl and glutaminyl peptides through steric and catalytic effects. , 2004, The journal of peptide research : official journal of the American Peptide Society.

[28]  H. Senyuva,et al.  Analysis and critical comparison of food allergen recalls from the European Union, USA, Canada, Hong Kong, Australia and New Zealand , 2016, Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment.

[29]  Christine H Parker,et al.  A Targeted LC-MS/MS Method for the Simultaneous Detection and Quantitation of Egg, Milk, and Peanut Allergens in Sugar Cookies. , 2018, Journal of AOAC International.

[30]  Scott H Sicherer,et al.  Clinical reviews in allergy and immunology , 2022 .

[31]  S. Kresovich,et al.  Genetic diversity of peanut (Arachis hypogaea L.) and its wild relatives based on the analysis of hypervariable regions of the genome , 2004, BMC Plant Biology.

[32]  C T Elliott,et al.  Is food allergen analysis flawed? Health and supply chain risks and a proposed framework to address urgent analytical needs. , 2016, The Analyst.

[33]  G. Agrawal,et al.  Proteomics analysis of mature seed of four peanut cultivars using two-dimensional gel electrophoresis reveals distinct differential expression of storage, anti-nutritional, and allergenic proteins , 2008 .

[34]  D. Gorbet,et al.  Fatty Acid and Amino Acid Profiles of Selected Peanut Cultivars and Breeding Lines , 1998 .

[35]  Steve L. Taylor,et al.  Allergenicity attributes of different peanut market types. , 2016, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[36]  T. Arnould,et al.  Development of a strategy for the quantification of food allergens in several food products by mass spectrometry in a routine laboratory. , 2019, Food chemistry.

[37]  Tong-Jen Fu,et al.  Effects of processing on the recovery of food allergens from a model dark chocolate matrix. , 2015, Food chemistry.