Paper matrix based array for rapid and sensitive optical detection of mercury ions using silver enhancement

AbstractThe authors describe a nitrocellulose membrane based paper matrix array for detection of mercury(II) with high throughput and repeatability. A thymine-rich signal reporting ssDNA probe labeled with gold nanoparticles (AuNPs) was designed, and detection is based on the strong T-Hg-T interaction which leads to a significant color change. In addition, catalytic signal amplification is accomplished by silver staining in order to enhance coloration. The method has a detection limit as low 0.5 ppt. This is at least 500-fold better than that of existing methods. By using an array, multiple sets of duplicate detections can be carried out simultaneously and in parallel. This improves the efficiency and accuracy of the assay. The method shows remarkable specificity since the signal of other competing metal ions is negligible compared to that of mercury(II) even at 100-fold higher concentration. The protocol was successfully applied to the determination of Hg(II) in spiked tap water and lake water samples. In our perception, this assay is a most promising tool for optical determination f ultra-low levels of Hg(II). Graphical abstractMetal deposition based signal enhancement strategy is successful applied in the paper-matrix based array protocol for rapid and accurate detection of mercury ions. 500-fold amplification effect is achieved for mercury ion detection with the detection limit of 0.5 ppt by bare-eye observation.

[1]  Weisheng Guo,et al.  Rapid and quantitative detection of prostate specific antigen with a quantum dot nanobeads-based immunochromatography test strip. , 2014, ACS applied materials & interfaces.

[2]  Juan Tang,et al.  DNA-based electrochemical determination of mercury(II) by exploiting the catalytic formation of gold amalgam and of silver nanoparticles , 2016, Microchimica Acta.

[3]  Yiping Cui,et al.  SERS detection and removal of mercury(II)/silver(I) using oligonucleotide-functionalized core/shell magnetic silica sphere@Au nanoparticles. , 2014, ACS applied materials & interfaces.

[4]  Yuan-Kai Wang,et al.  Simultaneous quantitative determination of multiple mycotoxins in cereal and feedstuff samples by a suspension array immunoassay. , 2013, Journal of agricultural and food chemistry.

[5]  Daniel Quesada-González,et al.  Nanoparticle-based lateral flow biosensors. , 2015, Biosensors & bioelectronics.

[6]  Yang Song,et al.  Multi-color quantum dot-based fluorescence immunoassay array for simultaneous visual detection of multiple antibiotic residues in milk. , 2015, Biosensors & bioelectronics.

[7]  T. G. Shrivastav,et al.  Selective capturing and detection of Salmonella typhi on polycarbonate membrane using bioconjugated quantum dots. , 2011, Talanta.

[8]  Sook Mei Khor,et al.  A colloidal gold-based lateral flow immunoassay for direct determination of haemoglobin A1c in whole blood , 2015 .

[9]  Paul Yager,et al.  Multiplexed enrichment and detection of malarial biomarkers using a stimuli-responsive iron oxide and gold nanoparticle reagent system. , 2012, ACS nano.

[10]  Haijuan Zeng,et al.  A new spot quality control for protein macroarray based on immunological detection. , 2015, Talanta.

[11]  Hu Tianyu,et al.  Aptamer-based aggregation assay for mercury(II) using gold nanoparticles and fluorescent CdTe quantum dots , 2016, Microchimica Acta.

[12]  Kang Zeng,et al.  Visual detection of Hg²⁺ in aqueous solution using gold nanoparticles and thymine-rich hairpin DNA probes. , 2011, Biosensors & bioelectronics.

[13]  D. Hofmann,et al.  Detailed‐atomistic molecular modeling of small molecule diffusion and solution processes in polymeric membrane materials , 2000 .

[14]  Guohua Zhao,et al.  A highly sensitive and wide-ranged electrochemical zinc(II) aptasensor fabricated on core-shell SiO2-Pt@meso-SiO2. , 2014, Biosensors & bioelectronics.

[15]  Xiliang Wang,et al.  Utilizing three monoclonal antibodies in the development of an immunochromatographic assay for simultaneous detection of sulfamethazine, sulfadiazine, and sulfaquinoxaline residues in egg and chicken muscle. , 2010, Analytical Chemistry.

[16]  Martin Moskovits,et al.  Visualizing chromatographic separation of metal ions on a surface-enhanced Raman active medium. , 2011, Nano letters.

[17]  Zhengbo Chen,et al.  Chitosan-functionalized gold nanoparticles for colorimetric detection of mercury ions based on chelation-induced aggregation , 2015, Microchimica Acta.

[18]  Takashi Fujimoto,et al.  MercuryII-mediated formation of thymine-HgII-thymine base pairs in DNA duplexes. , 2006, Journal of the American Chemical Society.

[19]  Shui-Tong Lee,et al.  Ordered Ag/Si nanowires array: wide-range surface-enhanced Raman spectroscopy for reproducible biomolecule detection. , 2013, Nano letters.

[20]  C. Toh,et al.  Membrane-based electrochemical nanobiosensor for the detection of virus. , 2009, Analytical chemistry.

[21]  Hengwei Lin,et al.  Paper-based colorimetric array test strip for selective and semiquantitative multi-ion analysis: simultaneous detection of Hg²⁺, Ag⁺, and Cu²⁺. , 2014, Analytical chemistry.

[22]  P. Gros,et al.  Hg(II) trace electrochemical detection on gold electrode: Evidence for chloride adsorption as the responsible for the broad baseline , 2013 .

[23]  Ying Wang,et al.  Ultrasensitive detection of mercury with a novel one-step signal amplified lateral flow strip based on gold nanoparticle-labeled ssDNA recognition and enhancement probes. , 2014, Biosensors & bioelectronics.

[24]  Lei Zheng,et al.  Immunochromatographic lateral flow strip for on-site detection of bisphenol A , 2013, Microchimica Acta.

[25]  Man Teng,et al.  Development of a lateral flow colloidal gold immunoassay strip for the rapid detection of enrofloxacin residues. , 2008, Journal of agricultural and food chemistry.

[26]  Xue-cheng Sun,et al.  Ultrasensitive detection and quantification of E. coli O157:H7 using a giant magnetoimpedance sensor in an open-surface microfluidic cavity covered with an antibody-modified gold surface , 2016, Microchimica Acta.

[27]  C. Elliott,et al.  Validation of a high-throughput immunobead array technique for multiplex detection of three foodborne pathogens in chicken products. , 2016, International journal of food microbiology.

[28]  Lei Zheng,et al.  Ultrasensitive and rapid screening of mercury(II) ions by dual labeling colorimetric method in aqueous samples and applications in mercury-poisoned animal tissues. , 2015, Analytica chimica acta.

[29]  R. Hagiwara,et al.  Electrochemical performance of hard carbon negative electrodes for ionic liquid-based sodium ion batteries over a wide temperature range , 2015 .

[30]  A. Denizli,et al.  Lysine-promoted colorimetric response of gold nanoparticles: a simple assay for ultrasensitive mercury(II) detection. , 2014, Analytical chemistry.

[31]  Lei Zheng,et al.  One-step signal amplified lateral flow strip biosensor for ultrasensitive and on-site detection of bisphenol A (BPA) in aqueous samples. , 2013, Biosensors & bioelectronics.

[32]  Chao Lin,et al.  Development of a one-step test strip for rapid screening of fumonisins B1, B2 and B3 in maize , 2012 .

[33]  Penelope C Ioannou,et al.  Oligonucleotide-functionalized gold nanoparticles as probes in a dry-reagent strip biosensor for DNA analysis by hybridization. , 2003, Analytical chemistry.

[34]  Otto S. Wolfbeis,et al.  Optical sensors for determination of heavy metal ions , 1997 .

[35]  M. Shamsipur,et al.  Highly selective aggregation assay for visual detection of mercury ion based on competitive binding of sulfur-doped carbon nanodots to gold nanoparticles and mercury ions , 2016, Microchimica Acta.

[36]  D. Pan,et al.  Rapid and ultrasensitive colorimetric detection of mercury(II) by chemically initiated aggregation of gold nanoparticles , 2015, Microchimica Acta.

[37]  Jingquan Liu,et al.  Lab-on-paper micro- and nano-analytical devices: Fabrication, modification, detection and emerging applications , 2016, Microchimica Acta.

[38]  Chad A Mirkin,et al.  Gold nanoparticle probes for the detection of nucleic acid targets. , 2006, Clinica chimica acta; international journal of clinical chemistry.

[39]  M. Fournier,et al.  High-sensitivity array analysis of gene expression for the early detection of disseminated breast tumor cells in peripheral blood , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[40]  Hualei Wang,et al.  A rapid immunochromatographic test strip for detecting rabies virus antibody , 2010, Journal of Virological Methods.

[41]  Minghua Wang,et al.  One-step fabrication of electrochemical biosensor based on DNA-modified three-dimensional reduced graphene oxide and chitosan nanocomposite for highly sensitive detection of Hg(II) , 2016 .

[42]  X. Xia,et al.  Versatile microfluidic droplets array for bioanalysis. , 2015, ACS applied materials & interfaces.