Carotenoid content of wild edible young shoots traditionally consumed in Spain (Asparagus acutifolius L., Humulus lupulus L., Bryonia dioica Jacq. and Tamus communis L.).

BACKGROUND Wild vegetables have traditionally been consumed as part of the Mediterranean diet, being valuable sources of nutrients and bioactive compounds. The objective of this work was to analyse the carotenoid content of the edible young shoots of four species (Asparagus acutifolius L., Humulus lupulus L., Bryonia dioica Jacq. and Tamus communis L.) as part of a wider study on the characterisation of the nutritional composition of wild edible plants commonly consumed in Spain. Samples were gathered from two locations in Central Spain for two consecutive years. RESULTS Lutein, β-carotene, neoxanthin and violaxanthin were quantified by high-performance liquid chromatography with a photodiode array detector. Median concentration ranges (μg g(-1) edible wet weight) were: β-carotene, 3.39-6.69, lutein, 5.44–19.13;neoxanthin, 5.17-17.37; and violaxanthin, 2.08-8.93. The highest carotenoid content was that of B. dioica (59.01 μg g(-1)) and the lowest was found in A. acutifolius (17.58 μg g(-1)) [corrected]. CONCLUSION Our results show that these wild young shoots are richer sources of carotenoids than many of the commercially available leafy vegetables.

[1]  M. Cámara,et al.  Fatty acids profiles of some Spanish wild vegetables , 2012, Food science and technology international = Ciencia y tecnologia de los alimentos internacional.

[2]  Ana Maria Carvalho,et al.  Tocopherol composition and antioxidant activity of Spanish wild vegetables , 2012, Genetic Resources and Crop Evolution.

[3]  M. Pardo-de-Santayana,et al.  Wild vegetables of the Mediterranean area as valuable sources of bioactive compounds , 2012, Genetic Resources and Crop Evolution.

[4]  D. Žnidarčič,et al.  Carotenoid and chlorophyll composition of commonly consumed leafy vegetables in Mediterranean countries. , 2011, Food chemistry.

[5]  E. Amante,et al.  Changes in carotenoids during processing and storage of pumpkin puree. , 2011, Food chemistry.

[6]  Ana Maria Carvalho,et al.  Use of HPLC–DAD–ESI/MS to profile phenolic compounds in edible wild greens from Portugal , 2011 .

[7]  Ana Maria Carvalho,et al.  Nutritional and in vitro antioxidant properties of edible wild greens in Iberian Peninsula traditional diet , 2011 .

[8]  F. Batič,et al.  Antioxidants in spring leaves of Oxalis acetosella L. , 2010 .

[9]  D. Rodriguez-Amaya.  Quantitative analysis, in vitro assessment of bioavailability and antioxidant activity of food carotenoids—A review , 2010 .

[10]  A. M. Carvalho.  Plantas y sabiduría popular del Parque Natural de Montesinho. Un estudio etnobotánico en Portugal , 2010 .

[11]  V. Böhm,et al.  Carotenoids: actual knowledge on food sources, intakes, stability and bioavailability and their protective role in humans. , 2009, Molecular nutrition & food research.

[12]  Jafar M. El-Qudah,et al.  Identification and Quantification of Major Carotenoids in Some Vegetables , 2009 .

[13]  Cintia Nanci Kobori,et al.  Uncultivated Brazilian Green Leaves are Richer Sources of Carotenoids than are Commercially Produced Leafy Vegetables , 2008, Food and nutrition bulletin.

[14]  Delia B. Rodriguez-Amaya,et al.  Updated Brazilian database on food carotenoids: Factors affecting carotenoid composition , 2008 .

[15]  M. El-Demerdash,et al.  Wild and semi-domesticated food plant consumption in seven circum-Mediterranean areas , 2008, International journal of food sciences and nutrition.

[16]  Javier Tardío,et al.  Wild food plants traditionally used in the province of Madrid, Central Spain , 2005, Economic Botany.

[17]  L. Massey.  Food oxalate: factors affecting measurement, biological variation, and bioavailability. , 2007, Journal of the American Dietetic Association.

[18]  Shih-Chuan Liu,et al.  Effects of domestic processing on steroidal saponins in Taiwanese yam cultivar (Dioscorea pseudojaponica Yamamoto). , 2006, Journal of agricultural and food chemistry.

[19]  A. Afolayan,et al.  The suitability of wild vegetables for alleviating human dietary deficiencies , 2006 .

[20]  L. P. Christensen,et al.  Content of carotenoids in commonly consumed Asian vegetables and stability and extractability during frying , 2006 .

[21]  M. Pardo-de-Santayana,et al.  Ethnobotanical review of wild edible plants in Spain , 2006 .

[22]  Elizabeth J Johnson,et al.  Carotenoid actions and their relation to health and disease. , 2005, Molecular aspects of medicine.

[23]  N. Pellegrini,et al.  Antioxidant characterization of some Sicilian edible wild greens. , 2005, Journal of agricultural and food chemistry.

[24]  R. Elliott.  Mechanisms of genomic and non-genomic actions of carotenoids. , 2005, Biochimica et biophysica acta.

[25]  Z. Moradi,et al.  THE AMOUNTS AND DISTRIBUTION OF DIOSGENIN AND SAPONIN AND THEIR CARBOHYDRATE MOIETY OF TAMUS COMMUNIS L. , 2005 .

[26]  A. Asai,et al.  An epoxide-furanoid rearrangement of spinach neoxanthin occurs in the gastrointestinal tract of mice and in vitro: formation and cytostatic activity of neochrome stereoisomers. , 2004, The Journal of nutrition.

[27]  F. Ertuğ.  Wild Edible Plants of the Bodrum Area (Muğla, Turkey) , 2004 .

[28]  M. Sagardoy,et al.  Changes in carotenoids and chlorophylls in fresh green asparagus (Asparagus officinalis L) stored under modified atmosphere packaging , 2004 .

[29]  W. Stahl,et al.  Antioxidant activity of carotenoids. , 2003, Molecular aspects of medicine.

[30]  P. Guarrera.  Food medicine and minor nourishment in the folk traditions of Central Italy (Marche, Abruzzo and Latium). , 2003, Fitoterapia.

[31]  P. Nakata.  Advances in our understanding of calcium oxalate crystal formation and function in plants , 2003 .

[32]  M. M. Rebolloso-Fuentes,et al.  Fatty acids and carotenoids from Stinging Nettle (Urtica dioica L.) , 2003 .

[33]  F. Granado,et al.  A fast, reliable and low-cost saponification protocol for analysis of carotenoids in vegetables. , 2001 .

[34]  M. Edelenbos,et al.  HPLC determination of chlorophyll and carotenoid pigments in processed green pea cultivars (Pisum sativum L.). , 2001, Journal of agricultural and food chemistry.

[35]  A. Naska,et al.  Guidelines for the intake of vegetables and fruit: the Mediterranean approach. , 2001, International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition.

[36]  L. Herrera-Estrella,et al.  Organic acid metabolism in plants: from adaptive physiology to transgenic varieties for cultivation in extreme soils. , 2000, Plant science : an international journal of experimental plant biology.

[37]  J. Deli,et al.  Carotenoid composition in the fruits of Asparagus officinalis. , 2000, Journal of agricultural and food chemistry.

[38]  J. Guil‐Guerrero,et al.  Lipids classes, fatty acids and carotenes of the leaves of six edible wild plants , 1999 .

[39]  J. Lampe,et al.  Health effects of vegetables and fruit: assessing mechanisms of action in human experimental studies. , 1999, The American journal of clinical nutrition.

[40]  J. J. Giménez-Martínez,et al.  NUTRITIONAL COMPOSITION OF WILD EDIBLE CRUCIFER SPECIES , 1999 .

[41]  J. G. Guerrero,et al.  Mineral Nutrient Composition of Edible Wild Plants , 1998 .

[42]  S. Gawlak,et al.  Characterization of ribosome-inactivating proteins isolated from Bryonia dioica and their utility as carcinoma-reactive immunoconjugates. , 1994, Bioconjugate chemistry.

[43]  F. Granado,et al.  Carotenoid composition in raw and cooked Spanish vegetables , 1992 .

[44]  F. Granado,et al.  An improved HPLC Method for the Separation of Fourteen Carotenoids, Including 15-/13- and 9-CIS-β-Carotene Isomers, Phytoene and Phytofluene , 1991 .

[45]  F. Khachik,et al.  Separation, identification, and quantification of carotenoids in fruits, vegetables and human plasma by high performance liquid chromatography , 1991 .

[46]  F. Granado,et al.  A Rapid Separation of Ten Carotenoids, Three Retinoids, Alpha-Tocopherol and d-Alpha-Tocopherol Acetate by High Performance Liquid Chromatography and its Application to Serum and Vegetable Samples , 1990 .

[47]  Y. Kimura,et al.  A Seasonal Variation of Carotenoid Composition in Green Leaves and Effect of Environmental Factors on It , 1990 .

[48]  P. Hylands,et al.  Bryonoside and bryoside—new triterpene glycosides from Bryonia dioica , 1982 .

[49]  E. D. Ritter,et al.  10 – CAROTENOID ANALYTICAL METHODS , 1981 .

[50]  G. Nano,et al.  [Partial synthesis of bryogenine. XII. Constituents of Bryonia dioica root]. , 1965, Il Farmaco; edizione scientifica.