Combined spectroscopic method for determining the fluorophore concentration in highly scattering media

The spectroscopic analysis of composition of highly scattering media, in particular, biological tissues, is associated with difficult interpretation of the spectrum of radiation subjected to both absorption and multiple scattering in a medium. The proposed technique of the combined analysis of spectra of laser radiation fluorescence and diffuse reflection by highly scattering biological media allows quantitative determination of concentrations of basic chromophores and fluorophores in tissues with simultaneous estimation of structural changes in objects under study. To determine the effect of structural parameters on the intensity of backscattered laser and fluorescent radiation, numerical simulation and physical modeling of the interaction of exciting and fluorescent radiation with highly scattering media are performed. The dependences of the measured signal on the concentrations of scatterers and fluorophore (protoporphyrin IX) under study are measured.

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