Enhanced specific antibody productivity of hybridomas resulting from hyperosmotic stress is cell line-specific

Hybridomas with non-growth-associated antibody production are thought to exhibit enhanced specific monoclonal antibody productivity (qMAb) when subjected to hyperosmotic stress. Two hybridoma cell lines exhibiting non-growth-associated antibody production, S3H5/γ2bA2 and DB9G8 hybridomas, are cultivated in a batch mode using hyperosmolar media resulting from sodium chloride addition. Their response to hyperosmotic stress regarding qMAb is quite different, though they show similar depression of cell growth in hyperosmolar media. The qMAb of S3H5/γ2bA2 cells in a hyperosmolar medium (396 mOsm/kg, 10% fetal bovine serum (FBS)) is enhanced by approximately 180% when compared with that in a standard medium (283 mOsm/kg, 10% FBS), while qMAb of DB9G8 cells in the same hyperosmolar medium is enhanced by only 10%. Thus, the enhanced qMAb of hybridomas exhibiting non-growth-associated antibody production resulting from hyperosmotic stress is cell line-specific.

[1]  W. Teo,et al.  Substantial overproduction of antibodies by applying osmotic pressure and sodium butyrate , 1993, Biotechnology and bioengineering.

[2]  Y. Bergman,et al.  Characterization of a carcinogen‐induced murine B lymphocyte cell line of C3H/eB origin , 1977, European journal of immunology.

[3]  B. Palsson,et al.  Effect of serum concentration on hybridoma cell growth and monoclonal antibody production at various initial cell densities. , 1989, Hybridoma.

[4]  Amit Varma,et al.  Production of monoclonal antibody using free‐suspended and immobilized hybridoma cells: Effect of serum , 1991, Biotechnology and bioengineering.

[5]  W M Miller,et al.  Determination of antibody content in live versus dead hybridoma cells: Analysis of antibody production in osmotically stressed cultures , 1992, Biotechnology and bioengineering.

[6]  B. Palsson,et al.  Effect of medium osmolarity on hybridoma growth, metabolism, and antibody production. , 1991, Biotechnology and Bioengineering.

[7]  W M Miller,et al.  Effects of Abrupt and Gradual Osmotic Stress on Antibody Production and Content in Hybridoma Cells That Differ in Production Kinetics , 1994, Biotechnology progress.

[8]  H. Blanch,et al.  A kinetic analysis of hybridoma growth and metabolism in continuous suspension culture on serum‐free medium , 1991, Biotechnology and bioengineering.

[9]  E. Jo,et al.  Step‐fortifications of nutrients in mammalian cell culture , 1993, Biotechnology and bioengineering.

[10]  T. Bender,et al.  Mapping epitopes on the insulin molecule using monoclonal antibodies , 1983, European journal of immunology.

[11]  Alvin W. Nienow,et al.  The effects of agitation intensity with and without continuous sparging on the growth and antibody production of hybridoma cells , 1989 .

[12]  B. Buckland,et al.  Monoclonal Antibody Process Development Using Medium Concentrates , 1994, Biotechnology progress.