Presynaptic action of neuropeptide Y in area CA1 of the rat hippocampal slice.

1. Neuropeptide tyrosine (neuropeptide Y, NPY), a recently isolated endogenous brain peptide, reduces the extracellular population spike evoked by stimulation of stratum radiatum in area CA1 of the in vitro rat hippocampal slice, without reducing the antidromically evoked population spike. To test the hypothesis that NPY acts presynaptically, intracellular recordings were made of pyramidal neurones of area CA1 in vitro. 2. Bath application of 10(‐6) M‐NPY causes a long‐lasting (1‐1.5 h), reversible reduction of the orthodromically evoked excitatory post‐synaptic potential (e.p.s.p.) recorded intracellularly from CA1 pyramidal neurones. This effect on the e.p.s.p. was dependent upon the concentration of NPY. 3. The resting membrane potential, slope input resistance, and action potential threshold, amplitude and duration of the CA1 pyramidal neurones were not affected by NPY. 4. The responses of CA1 pyramidal neurones to ionophoretic pulses of glutamate, applied to the dendrites during synaptic blockade, was also unaffected by NPY. 5. The evidence supports the hypothesis that NPY acts presynaptically in the CA1 region of hippocampus to reduce excitatory input to the pyramidal neurones.

[1]  J. Eccles,et al.  PATHWAY OF POSTSYNAPTIC INHIBITION IN THE HIPPOCAMPUS. , 1964, Journal of neurophysiology.

[2]  P. Andersen,et al.  Functional characteristics of unmyelinated fibres in the hippocampal cortex , 1978, Brain Research.

[3]  F. Edward Dudek,et al.  Local synaptic circuits in rat hippocampus: interactions between pyramidal cells , 1980, Brain Research.

[4]  P. Andersen,et al.  Possible mechanisms for long‐lasting potentiation of synaptic transmission in hippocampal slices from guinea‐pigs. , 1980, The Journal of physiology.

[5]  Q. Pittman,et al.  Somatostatin hyperpolarizes hippocampal pyramidal cells in vitro , 1981, Brain Research.

[6]  K. Tatemoto.  Neuropeptide Y: complete amino acid sequence of the brain peptide. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[7]  S. Bloom,et al.  Two novel related peptides, neuropeptide Y (NPY) and peptide YY (PYY) inhibit the contraction of the electrically stimulated mouse vas deferens , 1982, Neuropeptides.

[8]  R. Nicoll,et al.  Pharmacological evidence for two kinds of GABA receptors on rat hippocampal pyramidal cells studied in vitro , 1982, The Journal of physiology.

[9]  J. Lundberg,et al.  Pancreatic polypeptide family (APP, BPP, NPY and PYY) in relation to sympathetic vasoconstriction resistant to alpha-adrenoceptor blockade. , 1982, Acta physiologica Scandinavica.

[10]  D. Jacobowitz,et al.  The effects of pancreatic polypeptides and neuropeptide Y on the rat vas deferens , 1983, Peptides.

[11]  T. Crow,et al.  Neuropeptide Y distribution in human brain , 1983, Nature.

[12]  T. Crow,et al.  Neuropeptide Y distribution in the rat brain. , 1983, Science.

[13]  T. Hökfelt,et al.  Central administration of neuropeptide Y induces hypotension bradypnea and EEG synchronization in the rat. , 1983, Acta physiologica Scandinavica.

[14]  T. Hökfelt,et al.  High levels of neuropeptide Y in peripheral noradrenergic neurons in various mammals including man , 1983, Neuroscience Letters.

[15]  P. Emson,et al.  Neuropeptide Y: Cerebrovascular innervation an vasomotor effects in the cat , 1983, Neuroscience Letters.

[16]  P. Schwartzkroin,et al.  Passive Electrotonic Structure and Dendritic Properties of Hippocampal Neurons , 1984 .

[17]  P. Emson,et al.  Morphology, distribution, and synaptic relations of somatostatin- and neuropeptide Y-immunoreactive neurons in rat and monkey neocortex , 1984, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[18]  M. Mühlethaler,et al.  Contrasting effects of neurohypophysial peptides on pyramidal and non-pyramidal neurones in the rat hippocampus , 1984, Brain Research.

[19]  T. O'donohue,et al.  Coexistence of neuropeptide Y and somatostatin in rat and human cortical and rat hypothalamic neurons , 1984, Neuroscience Letters.

[20]  A. Franco‐Cereceda,et al.  Effects of neuropeptide Y (NPY) on mechanical activity and neurotransmission in the heart, vas deferens and urinary bladder of the guinea-pig. , 1984, Acta physiologica Scandinavica.

[21]  C. Wahlestedt,et al.  Neuropeptide Y potentiates the effect of various vasoconstrictor agents on rabbit blood vessels , 1984, British journal of pharmacology.

[22]  J. Polak,et al.  High concentrations of a novel peptide, neuropeptide Y, in the innervation of mouse and rat heart. , 1984, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[23]  J. Lundberg,et al.  Neuropeptide Y (NPY) depresses the secretion of 3H-noradrenaline and the contractile response evoked by field stimulation, in rat vas deferens. , 1984, Acta physiologica Scandinavica.

[24]  C. Ferris,et al.  Neuropeptide Y: Role in light-dark cycle entrainment of hamster circadian rhythms , 1984, Neuroscience Letters.

[25]  C. Wahlestedt,et al.  Neuropeptide Y co-exists and co-operates with noradrenaline in perivascular nerve fibers , 1984, Regulatory Peptides.

[26]  T. Hökfelt,et al.  Differential co-existence of neuropeptide Y (NPY)-like immunoreactivity with catecholamines in the central nervous system of the rat , 1984, Neuroscience.

[27]  S. Kalra,et al.  Norepinephrine-like effects of neuropeptide Y on LH release in the rat. , 1984, Life sciences.

[28]  E G Jones,et al.  Neuropeptide-containing neurons of the cerebral cortex are also GABAergic. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[29]  A. Saria,et al.  Evidence for specific neuropeptide Y-binding sites in rat brain synaptosomes. , 1984, European journal of pharmacology.

[30]  K. Lukowiak,et al.  Neuropeptide Y reduces orthodromically evoked population spike in rat hippocampal CA1 by a possibly presynaptic mechanism , 1985, Brain Research.

[31]  E. Potter.  Prolonged non-adrenergic inhibition of cardiac vagal action following sympathetic stimulation: Neuromodulation by neuropeptide Y? , 1985, Neuroscience Letters.

[32]  S. Lightman,et al.  Cardiovascular effects of neuropeptide Y in the nucleus tractus solitarius of rats: Relationship with noradrenaline and vasopressin , 1985, Peptides.

[33]  S. Leibowitz,et al.  Neuropeptide Y injected in the paraventricular hypothalamus: a powerful stimulant of feeding behavior. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[34]  T. O'donohue,et al.  The anatomy of neuropeptide-y-containing neurons in rat brain , 1985, Neuroscience.