Mg ion implantation on SLA-treated titanium surface and its effects on the behavior of mesenchymal stem cell.

Magnesium (Mg) is one of the most important ions associated with bone osseointegration. The aim of this study was to evaluate the cellular effects of Mg implantation in titanium (Ti) surfaces treated with sand blast using large grit and acid etching (SLA). Mg ions were implanted into the surface via vacuum arc source ion implantation. The surface morphology, chemical properties, and the amount of Mg ion release were evaluated by scanning electron microscopy (SEM), Auger electron spectroscopy (AES), Rutherford backscattering spectroscopy (RBS), and inductively coupled plasma-optical emission spectrometer (ICP-OES). Human mesenchymal stem cells (hMSCs) were used to evaluate cellular parameters such as proliferation, cytotoxicity, and adhesion morphology by MTS assay, live/dead assay, and SEM. Furthermore, osteoblast differentiation was determined on the basis of alkaline phosphatase (ALP) activity and the degree of calcium accumulation. In the Mg ion-implanted disk, 2.3×10(16) ions/cm(2) was retained. However, after Mg ion implantation, the surface morphology did not change. Implanted Mg ions were rapidly released during the first 7 days in vitro. The MTS assay, live/dead assay, and SEM demonstrated increased cell attachment and growth on the Mg ion-implanted surface. In particular, Mg ion implantation increased the initial cell adhesion, and in an osteoblast differentiation assay, ALP activity and calcium accumulation. These findings suggest that Mg ion implantation using the plasma source ion implantation (PSII) technique may be useful for SLA-treated Ti dental implants to improve their osseointegration capacity.

[1]  H. E. Kim,et al.  Ion-beam-assisted deposition (IBAD) of hydroxyapatite coating layer on Ti-based metal substrate. , 2000, Biomaterials.

[2]  Jin-Woo Park,et al.  Osteoblast response to magnesium ion-incorporated nanoporous titanium oxide surfaces. , 2010, Clinical oral implants research.

[3]  A. Wennerberg,et al.  Improved retention and bone-tolmplant contact with fluoride-modified titanium implants. , 2004, The International journal of oral & maxillofacial implants.

[4]  B. Kasemo.  Biocompatibility of titanium implants: surface science aspects. , 1983, The Journal of prosthetic dentistry.

[5]  A. Seifalian,et al.  Surface modification of POSS‐nanocomposite biomaterials using reactive oxygen plasma treatment for cardiovascular surgical implant applications , 2011, Biotechnology and applied biochemistry.

[6]  S. Moon,et al.  Electrochemical property and apatite formation of metal ion implanted titanium for medical implants , 2005 .

[7]  L. Cho,et al.  The effects of Mg-ion implantation and sandblasting on Porphyromonas gingivalis attachment. , 2012, Clinical oral implants research.

[8]  Daniel Perchy,et al.  In vitro evaluation of the surface effects on magnesium-yttrium alloy degradation and mesenchymal stem cell adhesion. , 2012, Journal of biomedical materials research. Part A.

[9]  N. Nardi,et al.  In Search of the In Vivo Identity of Mesenchymal Stem Cells , 2008, Stem cells.

[10]  R. Majeska,et al.  Studies on matrix vesicles isolated from chick epiphyseal cartilage. Association of pyrophosphatase and ATPase activities with alkaline phosphatase. , 1975, Biochimica et biophysica acta.

[11]  C. Kirkpatrick,et al.  Increased adhesion and activation of polymorphonuclear neutrophil granulocytes to endothelial cells under heavy metal exposure in vitro. , 1994, Pathobiology : journal of immunopathology, molecular and cellular biology.

[12]  L. Masi,et al.  Adhesion, growth, and matrix production by osteoblasts on collagen substrata , 1992, Calcified Tissue International.

[13]  M. Arlin.  Survival and success of sandblasted, large-grit, acid-etched and titanium plasma-sprayed implants: a retrospective study. , 2007, Journal.

[14]  D. Zaffe,et al.  Element release from titanium devices used in oral and maxillofacial surgery. , 2003, Biomaterials.

[15]  Ung-Jin Kim,et al.  Influence of macroporous protein scaffolds on bone tissue engineering from bone marrow stem cells. , 2005, Biomaterials.

[16]  K Asaoka,et al.  Early bone formation around calcium-ion-implanted titanium inserted into rat tibia. , 1997, Journal of biomedical materials research.

[17]  L Sennerby,et al.  Histologic evaluation of the bone integration of TiO(2) blasted and turned titanium microimplants in humans. , 2001, Clinical oral implants research.

[18]  A. Wennerberg,et al.  Surface characteristics of electrochemically oxidized implants and acid-etched implants: surface chemistry, morphology, pore configurations, oxide thickness, crystal structure, and roughness. , 2008, The International journal of oral & maxillofacial implants.

[19]  H. Donahue,et al.  Inhibiting gap junctional intercellular communication alters expression of differentiation markers in osteoblastic cells. , 1999, Bone.

[20]  H. E. Kim,et al.  Effects of ion beam-assisted deposition of hydroxyapatite on the osseointegration of endosseous implants in rabbit tibiae. , 2001, The International journal of oral & maxillofacial implants.

[21]  T. Albrektsson,et al.  Which surface properties enhance bone response to implants? Comparison of oxidized magnesium, TiUnite, and Osseotite implant surfaces. , 2006, The International journal of prosthodontics.

[22]  Yu-Tsai Wang,et al.  Effect of oxygen plasma immersion ion implantation treatment on corrosion resistance and cell adhesion of titanium surface. , 2011, Clinical oral implants research.

[23]  B. Boyan,et al.  Response of normal female human osteoblasts (NHOst) to 17beta-estradiol is modulated by implant surface morphology. , 2002, Journal of biomedical materials research.

[24]  Y. Sul,et al.  Biomechanical measurements of calcium-incorporated oxidized implants in rabbit bone: effect of calcium surface chemistry of a novel implant. , 2004, Clinical implant dentistry and related research.

[25]  T. Albrektsson,et al.  Bone reactions to oxidized titanium implants with electrochemical anion sulphuric acid and phosphoric acid incorporation. , 2002, Clinical implant dentistry and related research.

[26]  Chan-Jin Park,et al.  Bone response of Mg ion-implanted clinical implants with the plasma source ion implantation method. , 2010, Clinical oral implants research.

[27]  S. Spriano,et al.  Surface properties and cell response of low metal ion release Ti-6Al-7Nb alloy after multi-step chemical and thermal treatments. , 2005, Biomaterials.

[28]  T. Albrektsson,et al.  Optimum surface properties of oxidized implants for reinforcement of osseointegration: surface chemistry, oxide thickness, porosity, roughness, and crystal structure. , 2005, The International journal of oral & maxillofacial implants.

[29]  In-Seop Lee,et al.  The biocompatibility of SLA-treated titanium implants , 2008, Biomedical materials.

[30]  C. R. Howlett,et al.  Mechanisms of magnesium-stimulated adhesion of osteoblastic cells to commonly used orthopaedic implants. , 2002, Journal of biomedical materials research.

[31]  C. Knabe,et al.  In vitro investigation of titanium and hydroxyapatite dental implant surfaces using a rat bone marrow stromal cell culture system. , 2002, Biomaterials.

[32]  R. Brånemark,et al.  Morphological studies on machined implants of commercially pure titanium and titanium alloy (Ti6Al4V) in the rabbit. , 2009, Journal of biomedical materials research. Part B, Applied biomaterials.

[33]  H. Liu,et al.  The effects of surface and biomolecules on magnesium degradation and mesenchymal stem cell adhesion. , 2011, Journal of biomedical materials research. Part A.

[34]  S. Razzouk,et al.  Mesenchymal stem cells and their challenges for bone regeneration and osseointegration. , 2012, Journal of periodontology.