Utilization of laser induced breakdown spectroscopy for investigation of the metal accumulation in vegetal tissues

We report on the development and implementation of analytical methodology for investigating elemental accumulation in different layers within plant leaves, with in-situ spatial resolution mapping, exploiting the technique of LIBS. The spectrochemical analysis of lead-doped leaf samples is demonstrated to develop a real time identification procedure in order to complement other analytical techniques not lending themselves for spatial resolution analysis. Our findings suggest that with elevated levels of Pb within the plants transportation and storage of some nutrition elements is changed.

[1]  Yong Cai,et al.  A fern that hyperaccumulates arsenic , 2001, Nature.

[2]  Helmut H. Telle,et al.  Prospects of real-time single-particle biological aerosol analysis: A comparison between laser-induced breakdown spectroscopy and aerosol time-of-flight mass spectrometry , 2005 .

[3]  R. Leigh,et al.  Differential ion accumulation and ion fluxes in the mesophyll and epidermis of barley. , 2000, Plant physiology.

[4]  Sergei V. Kukhlevsky,et al.  Femtosecond laser spectrochemical analysis of plant samples , 2005 .

[5]  Francesco Flora,et al.  Mapping of the metal intake in plants by large-field X-ray microradiography and preliminary feasibility studies in microtomography , 2005 .

[6]  D. Beddows,et al.  Single-pollen analysis by laser-induced breakdown spectroscopy and Raman microscopy. , 2003, Applied optics.

[7]  M. Palmgren,et al.  A long way ahead: understanding and engineering plant metal accumulation. , 2002, Trends in plant science.

[8]  F. Flora,et al.  Monitoring of the heavy‐metal hyperaccumulation in vegetal tissues by X‐ray radiography and by femto‐second laser induced breakdown spectroscopy , 2007, Microscopy research and technique.

[9]  L. Kochian,et al.  Shoot biomass and zinc/cadmium uptake for hyperaccumulator and non-accumulator Thlaspi species in response to growth on a zinc-deficient calcareous soil , 2003 .

[10]  Timothy G. Rials,et al.  Analysis of preservative-treated wood by multivariate analysis of laser-induced breakdown spectroscopy spectra , 2005 .

[11]  J. Javier Laserna,et al.  Laser-induced plasma spectrometry: truly a surface analytical tool , 2004 .

[12]  Kevin L. McNesby,et al.  Investigation of statistics strategies for improving the discriminating power of laser-induced breakdown spectroscopy for chemical and biological warfare agent simulants , 2005 .