Suppression of benign prostate hyperplasia by Kaempferia parviflora rhizome

Background: Kaempferia parviflora rhizome is used as a folk medicine in Thailand for the treatment of various symptoms. In the present study, the inhibitory activities of extract from K. parviflora rhizome against 5α-reductase (5αR) were subjected. Furthermore, the effects of the extract from K. parviflorar hizome in benign prostate hyperplasia (BPH) were studied using the model mice. Materials and Methods: Preparations of extracts from the rhizomes of K. parviflora, Curcuma zedoaria and Zingiber officinale, and methoxyflavones isolated from K. parviflora was used for 5αR inhibition assay. The effects of K. parviflora extract on growth suppression for the prostates and seminal vesicles were performed based on the Hershberger's method. The K. parviflora extract was administered to castrated mice for 14 days. Results: K. parviflora extract showed more potent inhibitory activity on 5αR than C. zedoaria and Z. officinale extracts. The active principles were identified as 3,5,7,3’,4’-pentamethoxyflavone and 5,7,3’,4’-tetramethoxyflavone by activity guided fractionation. Furthermore, K. parviflora extract suppressed the weights of prostates and seminal vesicles in BPH model rats by daily administration for 14 days. Conclusion: These results indicate that K. parviflora extract can be a promising agent for the treatment of BPH.

[1]  H. Matsuda,et al.  Improvement in blood fluidity by Kaempferia parviflora rhizome , 2013, Journal of Natural Medicines.

[2]  Katsumasa Okamura,et al.  Promotion of Hair Growth by Rosmarinus officinalis Leaf Extract , 2013, Phytotherapy research : PTR.

[3]  T. Shimada,et al.  Polymethoxyflavonoids from Kaempferia parviflora induce adipogenesis on 3T3-L1 preadipocytes by regulating transcription factors at an early stage of differentiation. , 2012, Biological & pharmaceutical bulletin.

[4]  H. Matsuda,et al.  Effects of Ginseng Rhizome and Ginsenoside Ro on Testosterone 5α‐Reductase and Hair Re‐growth in Testosterone‐treated Mice , 2012, Phytotherapy research : PTR.

[5]  T. Shimada,et al.  Preventive effect of Kaempferia parviflora ethyl acetate extract and its major components polymethoxyflavonoid on metabolic diseases. , 2011, Fitoterapia.

[6]  T. Shinada,et al.  Xanthine oxidase inhibitory activities and crystal structures of methoxyflavones from Kaempferia parviflora rhizome. , 2011, Biological & pharmaceutical bulletin.

[7]  K. Ingkaninan,et al.  Effects of Kaempferia parviflora Wall. Ex Baker on endothelial dysfunction in streptozotocin-induced diabetic rats. , 2011, Journal of ethnopharmacology.

[8]  H. Sanada,et al.  Antiobesity effects of Kaempferia parviflora in spontaneously obese type II diabetic mice , 2011, Journal of Natural Medicines.

[9]  C. Yenjai,et al.  Cytotoxicity against KB and NCI-H187 cell lines of modified flavonoids from Kaempferia parviflora. , 2010, Bioorganic & medicinal chemistry letters.

[10]  U. Kokpol,et al.  Anticholinesterase activity of 7‐methoxyflavones isolated from Kaempferia parviflora , 2009, Phytotherapy research : PTR.

[11]  P. Venskutonis,et al.  The effect of Echinacea purpurea (L.) Moench extract on experimental prostate hyperplasia , 2009, Phytotherapy research : PTR.

[12]  T. Koyama,et al.  Banana Peel Extract Suppressed Prostate Gland Enlargement in Testosterone-Treated Mice , 2009, Bioscience, biotechnology, and biochemistry.

[13]  S. Tewtrakul,et al.  Anti-allergic activity of compounds from Kaempferia parviflora. , 2008, Journal of ethnopharmacology.

[14]  G. Gonzales,et al.  Dose-response effect of Red Maca (Lepidium meyenii) on benign prostatic hyperplasia induced by testosterone enanthate. , 2007, Phytomedicine : international journal of phytotherapy and phytopharmacology.

[15]  R. Yumoto,et al.  Modulation of function of multidrug resistance associated-proteins by Kaempferia parviflora extracts and their components. , 2007, European journal of pharmacology.

[16]  R. Kondo,et al.  Anti-androgenic activities of Ganoderma lucidum. , 2005, Journal of ethnopharmacology.

[17]  D. Kanjanapothi,et al.  Anti-gastric ulcer effect of Kaempferia parviflora. , 2005, Journal of ethnopharmacology.

[18]  W. Owens,et al.  OECD validation of the Hershberger assay in Japan: phase 2 dose response of methyltestosterone, vinclozolin, and p,p'-DDE. , 2003, Environmental health perspectives.

[19]  P. Kittakoop,et al.  Bioactive flavonoids from Kaempferia parviflora. , 2003, Fitoterapia.

[20]  M. Lieber,et al.  Long-term 6-year experience with finasteride in patients with benign prostatic hyperplasia. , 2003, Urology.

[21]  M. Kubo,et al.  Anti-androgenic and hair growth promoting activities of Lygodii spora (spore of Lygodium japonicum) I. Active constituents inhibiting testosterone 5alpha-reductase. , 2002, Biological & pharmaceutical bulletin.

[22]  M. Takatsuki,et al.  Strain sensitivity differences in the Hershberger assay. , 2001, Reproductive toxicology.

[23]  M. Kubo,et al.  Testosterone 5alpha-reductase inhibitory active constituents from Anemarrhenae Rhizoma. , 2001, Biological & pharmaceutical bulletin.

[24]  M. Kubo,et al.  Anti-androgenic activity of Myricae Cortex--isolation of active constituents from bark of Myrica rubra. , 2001, Biological & pharmaceutical bulletin.

[25]  G. Ooi,et al.  Enzyme assay for 5α-reductase Type 2 activity in the presence of 5α-reductase Type 1 activity in rat testis , 2000, The Journal of Steroid Biochemistry and Molecular Biology.

[26]  F. Habib,et al.  Serenoa repens (Permixon®): A 5α‐reductase types I and II inhibitor—new evidence in a coculture model of BPH , 1999, The Prostate.

[27]  S. Monti,et al.  Effects of long‐term treatment with Serenoa repens (Permixon®) on the concentrations and regional distribution of androgens and epidermal growth factor in benign prostatic hyperplasia , 1998, The Prostate.

[28]  G. Warne,et al.  Localization of functional domains in the androgen receptor , 1997, The Journal of Steroid Biochemistry and Molecular Biology.

[29]  S. J. Higgins,et al.  The endocrinology and developmental biology of the prostate. , 1987, Endocrine reviews.

[30]  Katsumasa Okamura,et al.  Inhibitory activities of Puerariae Flos against testosterone 5α-reductase and its hair growth promotion activities , 2011, Journal of Natural Medicines.

[31]  T. Murakami,et al.  Effects of Kaempferia parviflora extracts and their flavone constituents on P-glycoprotein function. , 2007, Journal of pharmaceutical sciences.

[32]  C. Roehrborn.  Benign prostatic hyperplasia: an overview. , 2005, Reviews in urology.

[33]  L. Marks.  5alpha-reductase: history and clinical importance. , 2004, Reviews in urology.

[34]  G. Ooi,et al.  Enzyme assay for 5alpha-reductase type 2 activity in the presence of 5alpha-reductase type 1 activity in rat testis. , 2000, The Journal of steroid biochemistry and molecular biology.