Toxic assessment of urban atmospheric particle-bound PAHs: relevance of composition and particle size in Barcelona (Spain).

Zebrafish embryotoxicity and dioxin-like activity levels were tested for particulate air samples from an urban background site in Barcelona (Spain). Samples were collected during 14 months, and maximal values for both biological activities corresponded to samples collected during late autumn months, correlating with elevated PAH levels. Vehicle and combustion emissions appeared as the potentially most toxic sources, whereas total PM mass and mineral content appeared to be poor predictors of the biological activity of the samples. Samples simultaneously collected at different particle size cut-offs (10, 2.5, and 1 μm) did not differ significantly in dioxin-like PAH levels and biological activity, indicating that the sub-micron particle fraction (PM1) concentrated essentially all observed toxicity. Our results support the need for a tighter control on sub-micron particle emissions and show that total PM mass and, particularly, PM10, may not fully characterize the toxic potential of air samples.

[1]  Daniel Krewski,et al.  Cardiovascular Mortality and Exposure to Airborne Fine Particulate Matter and Cigarette Smoke: Shape of the Exposure-Response Relationship , 2009, Circulation.

[2]  Christer Johansson,et al.  Cancer risk assessment, indicators, and guidelines for polycyclic aromatic hydrocarbons in the ambient air. , 2002, Environmental health perspectives.

[3]  Aldert H Piersma,et al.  Relative embryotoxicity of two classes of chemicals in a modified zebrafish embryotoxicity test and comparison with their in vivo potencies. , 2011, Toxicology in vitro : an international journal published in association with BIBRA.

[4]  N Künzli,et al.  Size fractionate particulate matter, vehicle traffic, and case-specific daily mortality in Barcelona, Spain. , 2009, Environmental science & technology.

[5]  L. Patrolecco,et al.  Occurrence of priority hazardous PAHs in water, suspended particulate matter, sediment and common eels (Anguilla anguilla) in the urban stretch of the River Tiber (Italy). , 2010, Chemosphere.

[6]  Mar Viana,et al.  A multidisciplinary approach to characterise exposure risk and toxicological effects of PM₁₀ and PM₂.₅ samples in urban environments. , 2012, Ecotoxicology and environmental safety.

[7]  Fabrice Cazier,et al.  Genotoxic potential of Polycyclic Aromatic Hydrocarbons-coated onto airborne Particulate Matter (PM 2.5) in human lung epithelial A549 cells. , 2008, Cancer letters.

[8]  Eliseo Monfort,et al.  Application of Optimally Scaled Target Factor Analysis for Assessing Source Contribution of Ambient PM10 , 2009, Journal of the Air & Waste Management Association.

[9]  J. Grimalt,et al.  Developmental effects of aerosols and coal burning particles in zebrafish embryos. , 2013, Environmental pollution.

[10]  S. Duggan,et al.  Exploratory investigation of the chemical characteristics and relative toxicity of ambient air particulates from two New Zealand cities. , 2009, The Science of the total environment.

[11]  Jeanne Garric,et al.  Development of a zebrafish 4-day embryo-larval bioassay to assess toxicity of chemicals. , 2006, Ecotoxicology and environmental safety.

[12]  U. Meyer,et al.  Human P-450 genes: evolution, regulation and possible role in carcinogenesis. , 1987, Biochemical Society transactions.

[13]  A. Valavanidis,et al.  Airborne Particulate Matter and Human Health: Toxicological Assessment and Importance of Size and Composition of Particles for Oxidative Damage and Carcinogenic Mechanisms , 2008, Journal of environmental science and health. Part C, Environmental carcinogenesis & ecotoxicology reviews.

[14]  B. Brunekreef,et al.  In vitro toxicity of particulate matter (PM) collected at different sites in the Netherlands is associated with PM composition, size fraction and oxidative potential - the RAPTES project , 2011, Particle and Fibre Toxicology.

[15]  D. Jerina,et al.  Carcinogenicity of Polycyclic Aromatic Hydrocarbons: The Bay-Region Theory , 1980 .

[16]  R. Xiao,et al.  Experimental study on the removal of PAHs using in-duct activated carbon injection. , 2005, Chemosphere.

[17]  R. Burnett,et al.  Lung cancer, cardiopulmonary mortality, and long-term exposure to fine particulate air pollution. , 2002, JAMA.

[18]  J. Schauer,et al.  Toxicity of ambient atmospheric particulate matter from the lake Michigan (USA) airshed to aquatic organisms , 2004, Environmental toxicology and chemistry.

[19]  R. Bissoli,et al.  Water Framework Directive 2000/60/EC. , 2008 .

[20]  W. Pryor,et al.  Quinoid redox cycling as a mechanism for sustained free radical generation by inhaled airborne particulate matter. , 2001, Free radical biology & medicine.

[21]  Wei Wang,et al.  Pulmonary toxicity study in rats with PM10 and PM2.5: Differential responses related to scale and composition , 2011 .

[22]  P. Ballesta,et al.  Seasonal and Daily Source Apportionment of Polycyclic Aromatic Hydrocarbon Concentrations in PM10 in a Semirural European Area , 2009 .

[23]  Damià Barceló,et al.  Evaluating the interactions of vertebrate receptors with persistent pollutants and antifouling pesticides using recombinant yeast assays , 2006, Analytical and bioanalytical chemistry.

[24]  D. Barceló,et al.  Detection of hormone receptor ligands in yeast by fluorogenic methods. , 2006, Talanta.

[25]  E. Canuel,et al.  Fate of atmospherically deposited polycyclic aromatic hydrocarbons (PAHs) in Chesapeake Bay. , 2001, Environmental science & technology.

[26]  Thomas W. Kirchstetter,et al.  On-Road Emissions of Particulate Polycyclic Aromatic Hydrocarbons and Black Carbon from Gasoline and Diesel Vehicles , 1998 .

[27]  V. Vasiliou,et al.  Role of the Ah Receptor and the Dioxin‐Inducible [Ah] Gene Battery in Toxicity, Cancer, and Signal Transduction a , 1993, Annals of the New York Academy of Sciences.

[28]  J. Kleinjans,et al.  Genotoxicity and physicochemical characteristics of traffic‐related ambient particulate matter , 2005, Environmental and molecular mutagenesis.

[29]  Miguel Luesma Castán,et al.  PAH in airborne particulate matter. Carcinogenic character of PM10 samples and assessment of the energy generation impact , 2011 .

[30]  K. Jones,et al.  Atmospheric dry deposition of persistent organic pollutants to the Atlantic and inferences for the global oceans. , 2004, Environmental science & technology.

[31]  J. Schauer,et al.  Seasonal and spatial relationship of chemistry and toxicity in atmospheric particulate matter using aquatic bioassays. , 2005, Environmental science & technology.

[32]  J. Klánová,et al.  PAHs on a west-to-east transect across the tropical Atlantic Ocean. , 2013, Environmental science & technology.

[33]  J. Grimalt,et al.  Influences of natural emission sources (wildfires and Saharan dust) on the urban organic aerosol in Barcelona (Western Mediterranean Basis) during a PM event , 2012, Environmental Science and Pollution Research.

[34]  T. D. de Kok,et al.  Toxicological assessment of ambient and traffic-related particulate matter: a review of recent studies. , 2006, Mutation research.

[35]  S. Gangamma,et al.  Particle Deposition in Human Respiratory Tract: Effect of Water- Soluble Fraction , 2006 .

[36]  Fulvio Amato,et al.  The Effects of Particulate Matter Sources on Daily Mortality: A Case-Crossover Study of Barcelona, Spain , 2011, Environmental health perspectives.

[37]  H. Handa,et al.  Aryl hydrocarbon receptor ligand activity of polycyclic aromatic ketones and polycyclic aromatic quinones , 2007, Environmental toxicology and chemistry.

[38]  E. Carraro,et al.  Application of semipermeable membrane device (SPMD) to assess air genotoxicity in an occupational environment. , 2009, Chemosphere.

[39]  X. Querol,et al.  Overview of the meteorology and transport patterns during the DAURE field campaign and their impact to PM observations , 2013 .

[40]  C. Kimmel,et al.  Stages of embryonic development of the zebrafish , 1995, Developmental dynamics : an official publication of the American Association of Anatomists.

[41]  J. Grimalt,et al.  Assessment of dioxin-like activity in ambient air particulate matter using recombinant yeast assays , 2011 .

[42]  T. Holsen,et al.  Dry Deposition of Particulate Polychlorinated Biphenyls and Polycyclic Aromatic Hydrocarbons to Lake Michigan , 1998 .

[43]  J. Dachs,et al.  Atmospheric occurrence and deposition of polycyclic aromatic hydrocarbons in the northeast tropical and subtropical Atlantic Ocean. , 2007, Environmental science & technology.

[44]  O. Jorba,et al.  Influence of the PBL scheme on high-resolution photochemical simulations in an urban coastal area over the Western Mediterranean , 2006 .

[45]  J. Dachs,et al.  Polycyclic aromatic hydrocarbons (PAHs) in the Mediterranean Sea: atmospheric occurrence, deposition and decoupling with settling fluxes in the water column. , 2012, Environmental pollution.

[46]  Fabrice Cazier,et al.  Prooxidant and proinflammatory potency of air pollution particulate matter (PM₂.₅₋₀.₃) produced in rural, urban, or industrial surroundings in human bronchial epithelial cells (BEAS-2B). , 2012, Chemical research in toxicology.

[47]  Charles A Miller.  Expression of the Human Aryl Hydrocarbon Receptor Complex in Yeast , 1997, The Journal of Biological Chemistry.

[48]  Monica Pandolfi,et al.  Quantifying road dust resuspension in urban environment by Multilinear Engine: A comparison with PMF2 , 2009 .

[49]  W. Thilly,et al.  Bacterial mutagenicity of urban organic aerosol sources in comparison to atmospheric samples. , 1994, Environmental science & technology.

[50]  R. Perry,et al.  A review of atmospheric polycyclic aromatic hydrocarbons: Sources, fate and behavior , 1991 .

[51]  L. Totten,et al.  Air—water exchange of polycyclic aromatic hydrocarbons in the New York—New Jersey, USA, Harbor Estuary , 2002, Environmental toxicology and chemistry.

[52]  Julieta M. Panzica-Kelly,et al.  Development of a zebrafish embryo teratogenicity assay and quantitative prediction model. , 2010, Birth defects research. Part B, Developmental and reproductive toxicology.

[53]  K. Jones,et al.  Latitudinal and seasonal capacity of the surface oceans as a reservoir of polychlorinated biphenyls. , 2004, Environmental pollution.

[54]  I. Nisbet,et al.  Toxic equivalency factors (TEFs) for polycyclic aromatic hydrocarbons (PAHs). , 1992, Regulatory toxicology and pharmacology : RTP.

[55]  W. Fritz.  WHO‐IARC Monographs on the evaluation of the carcinogenic risk of chemicals to humans. Vol. 32: Polynuclear Aromatic Compounds, Part 1, Chemical, Environmental and Experimental Data. 477 Seiten. International Agency for Research on Cancer, Lyon 1983. Preis: 80,‐ sfrs; 40.00 $ , 1985 .

[56]  James F. Pankow,et al.  Interdependence of the slopes and intercepts from log-log correlations of measured gas-particle paritioning and vapor pressure—I. theory and analysis of available data , 1992 .

[57]  R. Renne,et al.  Characterization of Mainstream Cigarette Smoke-Induced Biomarker Responses in ICR and C57Bl/6 Mice , 2004, Inhalation toxicology.