Nuclear localisation and pDNA condensation in non‐viral gene delivery

Non‐viral gene delivery vectors are multi‐component systems reflecting various functionalities required for effective cell transfection, including DNA condensation, promotion of cell membrane interactions and provision for subcellular targeting through endosomal escape and/or nuclear delivery. Elements mediating these functions will clearly display inter‐dependency. In this study we sought to explore the relationship within non‐viral vectors of condensation and nuclear localisation.

[1]  W. Traub,et al.  A comparative X‐ray study of a nucleoprotamine and DNA complexes with polylysine and polyarginine , 1972, Biopolymers.

[2]  J. A. Subirana,et al.  X-ray diffraction study of DNA complexes with arginine peptides and their relation to nucleoprotamine structure. , 1983, Journal of molecular biology.

[3]  Deutsches Krebsforschungszentrum,et al.  Nucleocytoplasmic Transport , 1986, Springer Berlin Heidelberg.

[4]  M. Cotten,et al.  Transferrin-polycation-DNA complexes: the effect of polycations on the structure of the complex and DNA delivery to cells. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[5]  J. Palau,et al.  Helical structure of basic proteins from spermatozoa. Comparison with model peptides. , 1993, European journal of biochemistry.

[6]  J. Hagstrom,et al.  Complexes of non-cationic liposomes and histone H1 mediate efficient transfection of DNA without encapsulation. , 1996, Biochimica et biophysica acta.

[7]  H. Schreier,et al.  Cationic liposome plasmid DNA complexes : In vitro cell entry and transgene expression augmented by synthetic signal peptides , 1996 .

[8]  K. Ulbrich,et al.  Characterization of vectors for gene therapy formed by self-assembly of DNA with synthetic block co-polymers. , 1996, Human gene therapy.

[9]  A T Hoogeveen,et al.  Efficacy of a peptide-based gene delivery system depends on mitotic activity. , 1996, Gene therapy.

[10]  F. Szoka,et al.  The influence of polymer structure on the interactions of cationic polymers with DNA and morphology of the resulting complexes , 1997, Gene Therapy.

[11]  L. Huang,et al.  In vivo gene transfer via intravenous administration of cationic lipid–protamine–DNA (LPD) complexes , 1997, Gene Therapy.

[12]  E. Schacht,et al.  Synthetic polymers for vectorial delivery of DNA : characterisation of polymer-DNA complexes by photon correlation spectroscopy and stability to nuclease degradation and disruption by polyanions in vitro , 1997 .

[13]  R Balhorn,et al.  AFM analysis of DNA-protamine complexes bound to mica. , 1997, Nucleic acids research.

[14]  J. Hughes,et al.  Nuclear localization signal peptides enhance cationic liposome-mediated gene therapy. , 1998, Journal of drug targeting.

[15]  C. K. Chan,et al.  Mutual exclusivity of DNA binding and nuclear localization signal recognition by the yeast transcription factor GAL4: implications for nonviral DNA delivery , 1998, Gene Therapy.

[16]  J. Bonadio,et al.  Stability of peptide-condensed plasmid DNA formulations. , 1998, Journal of pharmaceutical sciences.

[17]  J. Williams,et al.  Efficiency of cationic lipid-mediated transfection of polarized and differentiated airway epithelial cells in vitro and in vivo. , 1998, Human gene therapy.

[18]  A. Kabanov,et al.  Interpolyelectrolyte and block ionomer complexes for gene delivery: physico-chemical aspects. , 1998, Advanced drug delivery reviews.

[19]  Leaf Huang,et al.  Improved gene delivery formulations and expression systems for enhanced transaction efficiency , 1998 .

[20]  Leaf Huang,et al.  LPD lipopolyplex initiates a potent cytokine response and inhibits tumor growth , 1999, Gene Therapy.

[21]  I. Maclachlan,et al.  Cationic lipid-mediated transfection of cells in culture requires mitotic activity , 1999, Gene Therapy.

[22]  M. Kamihira,et al.  Enhancement of transfection efficiency by protamine in DDAB lipid vesicle-mediated gene transfer. , 1999, Journal of biochemistry.

[23]  J. Birchall,et al.  Physical stability and in-vitro gene expression efficiency of nebulised lipid-peptide-DNA complexes. , 2000, International journal of pharmaceutics.

[24]  M. Kamihira,et al.  Protamine-modified DDAB lipid vesicles promote gene transfer in the presence of serum. , 2001, Journal of biochemistry.

[25]  H. Mizuguchi,et al.  Nuclear targeting of DNA. , 2001, European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences.

[26]  D. Scherman,et al.  Critical assessment of the nuclear import of plasmid during cationic lipid‐mediated gene transfer , 2001, The journal of gene medicine.

[27]  D. Matthews,et al.  Enhanced cationic liposome-mediated transfection using the DNA-binding peptide μ (mu) from the adenovirus core , 2001, Gene Therapy.

[28]  R. Cartier,et al.  Utilization of synthetic peptides containing nuclear localization signals for nonviral gene transfer systems , 2002, Gene Therapy.

[29]  Andrew D. Miller,et al.  Biophysical characterization of the DNA binding and condensing properties of adenoviral core peptide mu. , 2002, Biochemistry.

[30]  N. Lamb,et al.  Macromolecular uptake is a spontaneous event during mitosis in cultured fibroblasts: implications for vector-dependent plasmid transfection. , 2002, Molecular biology of the cell.

[31]  J. Birchall,et al.  Preparation of dry powder dispersions for non‐viral gene delivery by freeze‐drying and spray‐drying , 2002, The journal of gene medicine.

[32]  J. Birchall,et al.  Enhanced Dispersibility and Deposition of Spray-dried Powders for Pulmonary Gene Therapy , 2003, Journal of drug targeting.

[33]  N. Minakawa,et al.  No enhancement of nuclear entry by direct conjugation of a nuclear localization signal peptide to linearized DNA. , 2003, Bioconjugate chemistry.

[34]  Xun Sun,et al.  Transfection efficiency of pORF lacZ plasmid lipopolyplex to hepatocytes and hepatoma cells. , 2004, World journal of gastroenterology.

[35]  J. Birchall,et al.  The use of absorption enhancers to enhance the dispersibility of spray‐dried powders for pulmonary gene therapy , 2005, The journal of gene medicine.

[36]  Y. Tabata,et al.  Preparation of poly(ethylene glycol)-introduced cationized gelatin as a non-viral gene carrier , 2005, Journal of biomaterials science. Polymer edition.

[37]  D. Crommelin,et al.  An NLS peptide covalently linked to linear DNA does not enhance transfection efficiency of cationic polymer based gene delivery systems , 2005, The journal of gene medicine.

[38]  J. Birchall,et al.  The use of amino acids to enhance the aerosolisation of spray‐dried powders for pulmonary gene therapy , 2005, The journal of gene medicine.

[39]  W. Colledge,et al.  Enhancement of gene delivery to human airway epithelial cells in vitro using a peptide from the polyoma virus protein VP1 , 2005, The journal of gene medicine.

[40]  Martin L Read,et al.  Enhanced gene transfer activity of peptide-targeted gene-delivery vectors , 2005, Journal of drug targeting.

[41]  Shiroh Futaki,et al.  Evaluation of the nuclear delivery and intra‐nuclear transcription of plasmid DNA condensed with µ (mu) and NLS‐µ by cytoplasmic and nuclear microinjection: a comparative study with poly‐L‐lysine , 2006 .

[42]  Sion A. Coulman,et al.  Minimally invasive cutaneous delivery of macromolecules and plasmid DNA via microneedles. , 2006, Current drug delivery.

[43]  S. Futaki,et al.  Evaluation of the nuclear delivery and intra-nuclear transcription of plasmid DNA condensed with micro (mu) and NLS-micro by cytoplasmic and nuclear microinjection: a comparative study with poly-L-lysine. , 2006, The journal of gene medicine.