Functional insights into long antisense noncoding RNA Kcnq1ot1 mediated bidirectional silencing

The prevailing view has been that noncoding antisense RNA primarily regulates the transcriptional activity of its sense counterpart, however, a subset of long noncoding antisense RNAs, such as Kcnq1ot1 and Air, have been shown to regulate the transcriptional silencing of multiple genes spread over several hundred kilobases on either side of their promoters. It is however unknown how these long RNAs regulate the transcriptional silencing of multiple genes. Our recent work demonstrated through exploiting an episomal-based system that the Kcnq1ot1 RNA harbors a silencing domain at its 5

[1]  P. Fraser,et al.  The role of long non-coding RNAs in chromatin structure and gene regulation: variations on a theme , 2008, Biological chemistry.

[2]  Paulo P. Amaral,et al.  The Eukaryotic Genome as an RNA Machine , 2008, Science.

[3]  J. Cavaille,et al.  Non‐coding RNAs in imprinted gene clusters , 2008, Biology of the cell.

[4]  Peter Fraser,et al.  Kcnq1ot1/Lit1 Noncoding RNA Mediates Transcriptional Silencing by Targeting to the Perinucleolar Region , 2008, Molecular and Cellular Biology.

[5]  Jong-Yeon Shin,et al.  Two distinct mechanisms of silencing by the KvDMR1 imprinting control region , 2008, The EMBO journal.

[6]  W. Bickmore,et al.  G9a Histone Methyltransferase Contributes to Imprinting in the Mouse Placenta , 2007, Molecular and Cellular Biology.

[7]  M. Oshimura,et al.  Suggestive evidence for chromosomal localization of non-coding RNA from imprinted LIT1 , 2007, Journal of Human Genetics.

[8]  Victor V Lobanenkov,et al.  Allele-Specific Binding of CTCF to the Multipartite Imprinting Control Region KvDMR1 , 2007, Molecular and Cellular Biology.

[9]  C. Kanduri,et al.  The length of the transcript encoded from the Kcnq1ot1 antisense promoter determines the degree of silencing , 2006, The EMBO journal.

[10]  S. Tilghman,et al.  Elongation of the Kcnq1ot1 transcript is required for genomic imprinting of neighboring genes. , 2006, Genes & development.

[11]  C. Kanduri,et al.  NF-Y Regulates the Antisense Promoter, Bidirectional Silencing, and Differential Epigenetic Marks of the Kcnq1 Imprinting Control Region* , 2004, Journal of Biological Chemistry.

[12]  K. Mitsuya,et al.  Imprinting on distal chromosome 7 in the placenta involves repressive histone methylation independent of DNA methylation , 2004, Nature Genetics.

[13]  C. Kanduri,et al.  An Antisense RNA Regulates the Bidirectional Silencing Property of the Kcnq1 Imprinting Control Region , 2004, Molecular and Cellular Biology.

[14]  P. Avner,et al.  The role of antisense transcription in the regulation of X-inactivation. , 2004, Current topics in developmental biology.

[15]  C. Kanduri,et al.  Bidirectional Silencing and DNA Methylation-sensitive Methylation-spreading Properties of the Kcnq1 Imprinting Control Region Map to the Same Regions* , 2003, The Journal of Biological Chemistry.

[16]  F. P. Villena,et al.  Genome imprinting regulated by the mouse Polycomb group protein Eed , 2003, Nature Genetics.

[17]  P. Soloway,et al.  Regional loss of imprinting and growth deficiency in mice with a targeted deletion of KvDMR1 , 2002, Nature Genetics.

[18]  C. Kanduri,et al.  A Differentially Methylated Imprinting Control Region within the Kcnq1 Locus Harbors a Methylation-sensitive Chromatin Insulator* , 2002, The Journal of Biological Chemistry.

[19]  D. Barlow,et al.  Quantitative genetics: Turning up the heat on QTL mapping , 2002, Nature Reviews Genetics.

[20]  Rudolf Jaenisch,et al.  Chromosomal silencing and localization are mediated by different domains of Xist RNA , 2002, Nature Genetics.