Lipid changes in tobacco cell suspensions following treatment with cellulase elicitor

Tobacco (Nicotians tabacum) KY14 cell cultures have previously been reported to produce capsidiol and other stress metabolites when treated with fungal elicitor or cellulase. Using a new high performance liquid chromatographic technique, we have measured the changes in sesquiterpene phytoalexins and membrane lipid classes thai occur upon elicitation of tobacco cell cultures with cellulase. Measurable levels of capsidiol and debneyol were found in the tobacco cells and in the culture medium after 8 h of elicitor treatment, with levels continuing to increase for up to 24 h. For the duration of the experiments, the levels of most of the galactolipids and phospholipids were found to decrease in elicited cells and increase in control cells. The most striking change was a rapid decrease in the level of digalactosyldiacylglycerol in elicited cells, to less than 10% of the level in control cells. Among the sterol lipid classes, the most notable changes occurred in the levels of sterol esters and acylated sterol glycosides, which increased significantly in elicited cells within 2 to 4 h after addition of cellulase, but remained unchanged in control cells. Free sterols and sterol glycosides declined slightly, while free fatty acids dropped to low levels 24 h after treatment of cells with cellulase. The present results and those of previous studies indicate that esterification of phytosterols may be a widespread response to environmental or chemical stress.