The antigen-binding characteristics of antibody pools of different relative affinity.

determinations, wepresent datawhichconfirmthevalidity ofaffinity measurementsderived fromextrapola-tion ofthe linear part ofsuch binding curves.MATERIALSANDMETHODSAntigensHumanserum albumin (HSA) was obtained from the Lister Institute of PreventiveMedicine and purified by gel filtration through Sephadex G-200. Radioiodination wascarried outbythemethodofMcFarlane (1958). DNP-HSAwasprepared bythe methodofEisen (1967). H3-E-DNP-L-lysine was prepared by the method of Eisen, Simms andPotter (1968).MiceInbred strains ofmice were immunized with antigen in saline