Detection of Cardiac Biomarker Troponin (cTnI) with Enhanced Immune Sensitivity using a Single Monoclonal Antibody

This work reports on using surface plasmon-enhanced fluorescence (SPFS) assay for the sensitive detection of cTnI at clinically relevant concentrations, using only one monoclonal antibody specific for one epitope. Moreover, it attempts to elucidate the role of the biointerface design by combined SPR and SPFS study in order to optimize the assay performance characteristics. This study is supported by protein modelling in order to visualize the dependence of the epitopes exposure on the protein orientation, which plays a significant role and it may provide important insights into the future development of cTnI immunoassays for accurate screening of CVD at early stage.

[1]  W. Knoll,et al.  Compact Grating-Coupled Biosensor for the Analysis of Thrombin. , 2019, ACS sensors.

[2]  R. El-Kased.  Immuno-analytical approach and its application for cardiac disease marker detection , 2018, Journal of immunoassay & immunochemistry.

[3]  X. He,et al.  Rapid and Sensitive Detection of Cardiac Troponin I for Point-of-Care Tests Based on Red Fluorescent Microspheres , 2018, Molecules.

[4]  Shalini Prasad,et al.  Cardiac troponin biosensors: where are we now? , 2018 .

[5]  C. Rodriguez-Emmenegger,et al.  Plasmonic Hepatitis B Biosensor for the Analysis of Clinical Saliva , 2017, Analytical chemistry.

[6]  B. Liedberg,et al.  Peptide Functionalized Gold Nanoparticles with Optimized Particle Size and Concentration for Colorimetric Assay Development: Detection of Cardiac Troponin I , 2016 .

[7]  Lin Wu,et al.  Exploiting Surface-Plasmon-Enhanced Light Scattering for the Design of Ultrasensitive Biosensing Modality. , 2016, Analytical chemistry.

[8]  J. Dostálek,et al.  Plasmonically amplified bioassay - Total internal reflection fluorescence vs. epifluorescence geometry. , 2016, Talanta.

[9]  Roger M. Leblanc,et al.  Recent Development of Cardiac Troponin i Detection , 2016 .

[10]  Rajesh R. Naik,et al.  Peptide Functionalized Gold Nanorods for the Sensitive Detection of a Cardiac Biomarker Using Plasmonic Paper Devices , 2015, Scientific Reports.

[11]  Feng Xu,et al.  Biomarker detection for disease diagnosis using cost-effective microfluidic platforms. , 2015, The Analyst.

[12]  Yang Zhang,et al.  The I-TASSER Suite: protein structure and function prediction , 2014, Nature Methods.

[13]  Jakub Dostalek,et al.  Plasmon-Enhanced Fluorescence Biosensors: a Review , 2013, Plasmonics.

[14]  A. Bereznikova,et al.  Epitope specificity of anti-cardiac troponin I monoclonal antibody 8I-7. , 2013, Clinical chemistry.

[15]  Qingge Xu,et al.  The impact of antibody selection on the detection of cardiac troponin I. , 2013, Clinica chimica acta; international journal of clinical chemistry.

[16]  J. Niazi,et al.  Biosensors for cardiac biomarkers detection: A review , 2012 .

[17]  Tsehai A. J. Grell,et al.  Rapid and sensitive detection of troponin I in human whole blood samples by using silver nanoparticle films and microwave heating. , 2011, Clinical chemistry.

[18]  Yang Zhang,et al.  I-TASSER: a unified platform for automated protein structure and function prediction , 2010, Nature Protocols.

[19]  M. Fischer,et al.  Amine coupling through EDC/NHS: a practical approach. , 2010, Methods in molecular biology.

[20]  W. Knoll,et al.  Long range surface plasmon-enhanced fluorescence spectroscopy for the detection of aflatoxin M1 in milk. , 2009, Biosensors & bioelectronics.

[21]  Fred S Apple,et al.  Assessment of the multiple-biomarker approach for diagnosis of myocardial infarction in patients presenting with symptoms suggestive of acute coronary syndrome. , 2009, Clinical chemistry.

[22]  W. Knoll,et al.  New concepts with surface plasmons and nano-biointerfaces , 2008 .

[23]  Fred S Apple,et al.  Universal definition of myocardial infarction. , 2007, Journal of the American College of Cardiology.

[24]  Yang Zhang,et al.  I-TASSER server for protein 3D structure prediction , 2008, BMC Bioinformatics.

[25]  J. Dostálek,et al.  Multichannel SPR biosensor for detection of endocrine-disrupting compounds , 2007, Analytical and bioanalytical chemistry.

[26]  Holger H. Hoos,et al.  A replica exchange Monte Carlo algorithm for protein folding in the HP model , 2007, BMC Bioinformatics.

[27]  W. Knoll,et al.  Long Range Surface Plasmons for Observation of Biomolecular Binding Events at Metallic Surfaces , 2007 .

[28]  Jean-Philippe Charrier,et al.  Isoelectric point determination of cardiac troponin I forms present in plasma from patients with myocardial infarction. , 2007, Clinica chimica acta; international journal of clinical chemistry.

[29]  Lars Edenbrandt,et al.  Direct hospital costs of chest pain patients attending the emergency department: a retrospective study , 2006, BMC emergency medicine.

[30]  D. Cook,et al.  Elevated cardiac troponin levels in critically ill patients: prevalence, incidence, and outcomes. , 2006, American journal of critical care : an official publication, American Association of Critical-Care Nurses.

[31]  A. Jaffe,et al.  Troponin: the biomarker of choice for the detection of cardiac injury , 2005, Canadian Medical Association Journal.

[32]  A. Katrukha.  Antibody Selection Strategies in Cardiac Troponin Assays , 2003 .

[33]  A. Go,et al.  The prognostic value of troponin in patients with non-ST elevation acute coronary syndromes: a meta-analysis. , 2001, Journal of the American College of Cardiology.

[34]  Wolfgang Knoll,et al.  Surface-Plasmon Field-Enhanced Fluorescence Spectroscopy , 2000 .

[35]  W. Knoll,et al.  Interfaces and thin films as seen by bound electromagnetic waves. , 1998, Annual review of physical chemistry.

[36]  K. Pulkki,et al.  Troponin I is released in bloodstream of patients with acute myocardial infarction not in free form but as complex. , 1997, Clinical chemistry.