Scanning Fiber Endoscope with multiple fluorescence-reflectance imaging channels for guiding biopsy

Fluorescence-labeled molecular probes can be used during endoscopy for early cancer detection. As many tumors express multiple cell surface markers and these molecular signatures are heterogeneous across patients, simultaneous imaging of numerous different molecular targets is important for increasing the sensitivity of early cancer diagnosis and personalized treatment. For this purpose, a wide-field, multi-spectral fluorescence-reflectance scanning fiber endoscope (SFE) has been developed. Using a set of calibrated fluorescent test targets at in vivo dye concentration, algorithms and methodologies were developed and demonstrated. Preliminary results showed the promise of fluorescence molecular imaging in clinical applications using the multi-spectral SFE.

[1]  Thomas D. Wang,et al.  Targeted Imaging of Esophageal Neoplasia with a Fluorescently Labeled Peptide: First-in-Human Results , 2013, Science Translational Medicine.

[2]  H Stepp,et al.  Detection of early bladder cancer by 5-aminolevulinic acid induced porphyrin fluorescence. , 1996, The Journal of urology.

[3]  Thomas D. Wang,et al.  Targeted endoscopic imaging. , 2009, Gastrointestinal endoscopy clinics of North America.

[4]  Ann M Gillenwater,et al.  Optical molecular imaging of multiple biomarkers of epithelial neoplasia: epidermal growth factor receptor expression and metabolic activity in oral mucosa. , 2012, Translational oncology.

[5]  Hisataka Kobayashi,et al.  Multicolor in vivo targeted imaging to guide real‐time surgery of HER2‐positive micrometastases in a two‐tumor coincident model of ovarian cancer , 2009, Cancer science.

[6]  James M Olson,et al.  In vivo bio-imaging using chlorotoxin-based conjugates. , 2011, Current pharmaceutical design.

[7]  Eric J. Seibel,et al.  Targeted detection of murine colonic dysplasia in vivo with flexible multispectral scanning fiber endoscopy , 2012, Photonics West - Biomedical Optics.

[8]  Miriam Scadeng,et al.  Surgery with molecular fluorescence imaging using activatable cell-penetrating peptides decreases residual cancer and improves survival , 2010, Proceedings of the National Academy of Sciences.

[9]  P. Low,et al.  Intraoperative tumor-specific fluorescence imaging in ovarian cancer by folate receptor-α targeting: first in-human results , 2011, Nature Medicine.

[10]  Thomas D. Wang,et al.  Future and advances in endoscopy , 2011, Journal of biophotonics.

[11]  Christopher H Contag,et al.  Detection of colonic dysplasia in vivo using a targeted heptapeptide and confocal microendoscopy. , 2008, Nature medicine.

[12]  Brian C Wilson,et al.  Detection and treatment of dysplasia in Barrett's esophagus: a pivotal challenge in translating biophotonics from bench to bedside. , 2007, Journal of biomedical optics.

[13]  John V Frangioni,et al.  New technologies for human cancer imaging. , 2008, Journal of clinical oncology : official journal of the American Society of Clinical Oncology.

[14]  Eric J. Seibel,et al.  Mitigating fluorescence spectral overlap in wide-field endoscopic imaging , 2013, Journal of biomedical optics.

[15]  Thomas D. Wang,et al.  Exogenous Molecular Probes for Targeted Imaging in Cancer: Focus on Multi-modal Imaging , 2010, Cancers.

[16]  Sylvain Gioux,et al.  Design and characterization of an optimized simultaneous color and near-infrared fluorescence rigid endoscopic imaging system , 2013, Journal of biomedical optics.

[17]  Richard S. Johnston,et al.  Multi-spectral scanning fiber endoscope with concurrent autofluorescence mitigation for enhanced target-to-background ratio imaging , 2014, Photonics West - Biomedical Optics.

[18]  Chenying Yang,et al.  Color-matched and fluorescence-labeled esophagus phantom and its applications , 2013, Journal of biomedical optics.