Molecular imaging of proteins in tissues by mass spectrometry

Imaging MS (IMS) is an emerging technology that permits the direct analysis and determination of the distribution of molecules in tissue sections. Biological molecules such as proteins, peptides, lipids, xenobiotics, and metabolites can be analyzed in a high-throughput manner with molecular specificity not readily achievable through other means. Tissues are analyzed intact and thus spatial localization of molecules within a tissue is preserved. Several studies are presented that focus on the unique types of information obtainable by IMS, such as Aβ isoform distributions in Alzheimer's plaques, protein maps in mouse brain, and spatial protein distributions in human breast carcinoma. The analysis of a biopsy taken 100 years ago from a patient with amyloidosis illustrates the use of IMS with formalin-fixed tissues. Finally, the registration and correlation of IMS with MRI is presented.

[1]  P. Chaurand,et al.  Enhancement of protein sensitivity for MALDI imaging mass spectrometry after chemical treatment of tissue sections , 2008, Journal of the American Society for Mass Spectrometry.

[2]  Richard M Caprioli,et al.  Imaging mass spectrometry reveals unique protein profiles during embryo implantation. , 2008, Endocrinology.

[3]  P. Chaurand,et al.  Monitoring Mouse Prostate Development by Profiling and Imaging Mass Spectrometry*S , 2008, Molecular & Cellular Proteomics.

[4]  Benoit M Dawant,et al.  Integrating spatially resolved three-dimensional MALDI IMS with in vivo magnetic resonance imaging , 2008, Nature Methods.

[5]  Ariel Y Deutch,et al.  Imaging mass spectrometry of proteins and peptides: 3D volume reconstruction , 2008, Nature Methods.

[6]  Patrick Ducoroy,et al.  Specific MALDI imaging and profiling for biomarker hunting and validation: fragment of the 11S proteasome activator complex, Reg alpha fragment, is a new potential ovary cancer biomarker. , 2007, Journal of proteome research.

[7]  Gerhard Jakse,et al.  Identifying prostate carcinoma by MALDI-Imaging. , 2007, International journal of molecular medicine.

[8]  Richard A Yost,et al.  Automated MALDI matrix deposition method with inkjet printing for imaging mass spectrometry. , 2007, Analytical chemistry.

[9]  L. McDonnell,et al.  Tools and strategies for visualization of large image data sets in high-resolution imaging mass spectrometry. , 2007, The Review of scientific instruments.

[10]  A. Woods,et al.  Direct MALDI-MS analysis of cardiolipin from rat organs sections , 2007, Journal of the American Society for Mass Spectrometry.

[11]  Sander R. Piersma,et al.  High-resolution MALDI imaging mass spectrometry allows localization of peptide distributions at cellular length scales in pituitary tissue sections , 2007 .

[12]  R. Caprioli,et al.  Identification of proteins directly from tissue: in situ tryptic digestions coupled with imaging mass spectrometry. , 2007, Journal of mass spectrometry : JMS.

[13]  P. Chaurand,et al.  Processing MALDI Mass Spectra to Improve Mass Spectral Direct Tissue Analysis. , 2007, International journal of mass spectrometry.

[14]  Hanno Langen,et al.  Biomarker Discovery by Imaging Mass Spectrometry , 2006, Molecular & Cellular Proteomics.

[15]  Richard M Caprioli,et al.  A Novel Histology-directed Strategy for MALDI-MS Tissue Profiling That Improves Throughput and Cellular Specificity in Human Breast Cancer* , 2006, Molecular & Cellular Proteomics.

[16]  Richard M. Caprioli,et al.  Molecular Assessment of the Tumor Protein Microenvironment Using Imaging Mass Spectrometry , 2006 .

[17]  P. Chaurand,et al.  Selective Profiling of Proteins in Lung Cancer Cells from Fine-Needle Aspirates by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry , 2006, Clinical Cancer Research.

[18]  R. Caprioli,et al.  Direct molecular analysis of whole-body animal tissue sections by imaging MALDI mass spectrometry. , 2006, Analytical chemistry.

[19]  M. Setou,et al.  A Novel Approach to in situ Proteome Analysis Using Chemical Inkjet Printing Technology and MALDI-QIT-TOF Tandem Mass Spectrometer , 2006 .

[20]  M. Setou,et al.  Thin Sectioning Improves the Peak Intensity and Signal-to-Noise Ratio in Direct Tissue Mass Spectrometry , 2006 .

[21]  Richard M Caprioli,et al.  Automated acoustic matrix deposition for MALDI sample preparation. , 2006, Analytical chemistry.

[22]  Walter A. Korfmacher,et al.  Investigation of the profiling depth in matrix-assisted laser desorption/ionization imaging mass spectrometry. , 2006, Rapid communications in mass spectrometry : RCM.

[23]  Yu Shyr,et al.  Proteomic patterns of preinvasive bronchial lesions. , 2005, American journal of respiratory and critical care medicine.

[24]  Yu Shyr,et al.  Proteomic-based prognosis of brain tumor patients using direct-tissue matrix-assisted laser desorption ionization mass spectrometry. , 2005, Cancer research.

[25]  R. Caprioli,et al.  Combination detergent/MALDI matrix: functional cleavable detergents for mass spectrometry. , 2005, Analytical chemistry.

[26]  Richard M Caprioli,et al.  Early Changes in Protein Expression Detected by Mass Spectrometry Predict Tumor Response to Molecular Therapeutics , 2004, Cancer Research.

[27]  Dean Billheimer,et al.  Integrating histology and imaging mass spectrometry. , 2004, Analytical chemistry.

[28]  PierreChaurand,et al.  Exploring the Potential of Cryodetectors for the Detection of Matrix-Assisted Laser Desorption/Ionization Produced Ions: Application to Profiling and Imaging Mass Spectrometry , 2004 .

[29]  Baogang J. Xu,et al.  Profiling and imaging proteins in the mouse epididymis by imaging mass spectrometry , 2003, Proteomics.

[30]  Michelle L. Reyzer,et al.  Direct tissue analysis using matrix-assisted laser desorption/ionization mass spectrometry: practical aspects of sample preparation. , 2003, Journal of mass spectrometry : JMS.

[31]  Jonathan V Sweedler,et al.  Spatial profiling invertebrate ganglia using MALDI MS , 2003, Journal of the American Society for Mass Spectrometry.

[32]  M. Stoeckli,et al.  Imaging mass spectrometry: A new technology for the analysis of protein expression in mammalian tissues , 2001, Nature Medicine.

[33]  R. Caprioli,et al.  Molecular imaging of biological samples: localization of peptides and proteins using MALDI-TOF MS. , 1997, Analytical chemistry.

[34]  Ronald C. Beavis,et al.  Matrix-assisted laser desorption/ionization mass spectrometry of biopolymers. , 1991, Analytical chemistry.

[35]  P. Westermark,et al.  Demonstration of amyloid protein AA in old museum specimens. , 1984, Archives of pathology & laboratory medicine.