Effect of Nd:YAG laser-nitriding-treated titanium nitride surface over Ti6Al4V substrate on the activity of MC3T3-E1 cells.

Ti6Al4V discs with a thickness of 2.5 mm and dimensions of 15 × 15 mm2 were fabricated. The titanium nitride (TiN) surface was formed via Nd:YAG laser-nitriding. A sandblast acid-etched (SA) surface was used as a control. Scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), and surface roughness tests were conducted to study the surface and cross-section morphologies as well as the properties of TiN and SA surfaces. MC3T3-E1 osteoblast-like cells were cultured on the TiN and SA surfaces to evaluate the effect of TiN surface on cellular behaviors, including attachment, proliferation and differentiation. Morphological testing results revealed that the cross-section of TiN exhibited dendritic crystallization without cracking. The proliferation and differentiation of MC3T3-E1 cells on the laser-nitriding TiN surface were significantly increased compared to those cultured on SA surface. These findings suggested that the TiN surface generated from Nd:YAG laser-nitriding were favorable for the proliferation and differentiation of MC3T3-E1 cells, which is significant for implant surface modification.

[1]  Raffaella Aversa,et al.  Biological response of human bone marrow stromal cells to sandblasted titanium nitride-coated implant surfaces , 2008, Journal of materials science. Materials in medicine.

[2]  L. Rasmusson,et al.  A 10-year follow-up study of titanium dioxide-blasted implants. , 2005, Clinical implant dentistry and related research.

[3]  Koichi Ito,et al.  Treatment of titanium dental implants with three piezoelectric ultrasonic scalers: an in vivo study. , 2007, Journal of periodontology.

[4]  In-Seop Lee,et al.  The biocompatibility of SLA-treated titanium implants , 2008, Biomedical materials.

[5]  K. Baba,et al.  Abrasion resistance of titanium nitride coatings formed on titanium by ion-beam-assisted deposition. , 2005, Journal of oral rehabilitation.

[6]  E. Czarnowska,et al.  Improvement of titanium alloy for biomedical applications by nitriding and carbonitriding processes under glow discharge conditions , 2000, Journal of materials science. Materials in medicine.

[7]  L. Cooper,et al.  Formation of mineralizing osteoblast cultures on machined, titanium oxide grit-blasted, and plasma-sprayed titanium surfaces. , 1999, The International journal of oral & maxillofacial implants.

[8]  J. Celis,et al.  Comparison of wear and corrosion wear of TiN coatings under uni- and bidirectional sliding , 1999 .

[9]  D. Brunette,et al.  Substratum surface topography alters cell shape and regulates fibronectin mRNA level, mRNA stability, secretion and assembly in human fibroblasts. , 1995, Journal of cell science.

[10]  Y. Gong,et al.  Biomimetic coating of compound titania and hydroxyapatite on titanium. , 2007, Journal of biomedical materials research. Part A.

[11]  R. Singer,et al.  Chemical vapor deposition of titanium nitride on carbon fibres as a protective layer in metal matrix composites , 1997 .

[12]  R. Mengel,et al.  An in vitro study of the treatment of implant surfaces with different instruments. , 1998, The International journal of oral & maxillofacial implants.

[13]  Hua-ming Wang,et al.  Wear resistance of a laser surface alloyed Ti–6Al–4V alloy , 2000 .

[14]  Torsten Jemt,et al.  Implant treatment in the edentulous mandible: a prospective study on Brånemark system implants over more than 20 years. , 2003, The International journal of prosthodontics.

[15]  Ann Wennerberg,et al.  Oral implant surfaces: Part 1--review focusing on topographic and chemical properties of different surfaces and in vivo responses to them. , 2004, The International journal of prosthodontics.

[16]  M. Yamauchi,et al.  Titanium surface topography affects collagen biosynthesis of adherent cells. , 2011, Bone.

[17]  P. Krebsbach,et al.  Isolation and Characterization of MC3T3‐E1 Preosteoblast Subclones with Distinct In Vitro and In Vivo Differentiation/Mineralization Potential , 1999, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[18]  R. Mengel,et al.  The treatment of uncoated and titanium nitride-coated abutments with different instruments. , 2004, The International journal of oral & maxillofacial implants.

[19]  T. Yue,et al.  Excimer laser surface treatment of Ti–6Al–4V alloy for corrosion resistance enhancement , 2002 .

[20]  L F Cooper,et al.  A role for surface topography in creating and maintaining bone at titanium endosseous implants. , 2000, The Journal of prosthetic dentistry.

[21]  Yun Tian,et al.  Research progress on laser surface modification of titanium alloys , 2005 .