Interaction of antibiotics with functional sites in 16S ribosomal RNA

Chemical footprinting shows that several classes of antibiotics (streptomycin, tetracycline, spectinomycin, edeine, hygromycin and the neomycins) protect concise sets of highly conserved nucleotides in 16S ribosomal RNA when bound to ribosomes. These findings have strong implications for the mechanism of action of these antibiotics and for the assignment of functions to specific structural features of 16S rRNA.

[1]  A. Kaji,et al.  Binding of specific sRNA to 30S ribosomal subunits: effect of 50S ribosomal subunits. , 1966, Proceedings of the National Academy of Sciences of the United States of America.

[2]  T. Ikemura,et al.  Specific inactivation of 16S ribosomal RNA induced by colicin E3 in vivo. , 1971, Proceedings of the National Academy of Sciences of the United States of America.

[3]  D. Vazquez.  Inhibitors of Protein Biosynthesis , 1979, Molecular Biology Biochemistry and Biophysics.

[4]  C. Sigmund,et al.  Antibiotic resistance mutations in 16S and 23S ribosomal RNA genes of Escherichia coli. , 1984, Nucleic acids research.

[5]  S. K. Smailov,et al.  Effect of streptomycin on the stoichiometry of GTP hydrolysis in a poly(U)‐dependent cell‐free translation system , 1985, FEBS letters.

[6]  A. Okuyama,et al.  Inhibition by kasugamycin of initiation complex formation on 30S ribosomes. , 1971, Biochemical and biophysical research communications.

[7]  R. Brimacombe,et al.  The tertiary folding of Escherichia coli 16S RNA, as studied by in situ intra-RNA cross-linking of 30S ribosomal subunits with bis-(2-chloroethyl)-methylamine. , 1986, Nucleic acids research.

[8]  E. Blackburn,et al.  The nucleotide sequence of the 17S ribosomal RNA gene of Tetrahymena thermophila and the identification of point mutations resulting in resistance to the antibiotics paromomycin and hygromycin. , 1985, The Journal of biological chemistry.

[9]  J. Ofengand,et al.  Covalent crosslinking of tRNA1Val to 16S RNA at the ribosomal P site: identification of crosslinked residues. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[10]  H. Khorana,et al.  A further study of misreading of codons induced by streptomycin and neomycin using ribopolynucleotides containing two nucleotides in alternating sequence as templates. , 1966, Journal of molecular biology.

[11]  A. Bollen,et al.  Ribosomal Protein Conferring Sensitivity to the Antibiotic Spectinomycin in Escherichia coli , 1969, Science.

[12]  M. Yarus,et al.  Transfer RNA selection at the ribosomal A and P sites. , 1979, Journal of molecular biology.

[13]  H. Noller,et al.  Rapid chemical probing of conformation in 16 S ribosomal RNA and 30 S ribosomal subunits using primer extension. , 1986, Journal of molecular biology.

[14]  R. Wagner,et al.  E. coli ribosomes with a C912 to U base change in the 16S rRNA are streptomycin resistant. , 1986, The EMBO journal.

[15]  B. D. Davis,et al.  Selective inhibition of initiating ribosomes by spectinomycin. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[16]  D. Peattie,et al.  Direct chemical method for sequencing RNA. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[17]  R. Thompson,et al.  Effect of Mg2+ concentration, polyamines, streptomycin, and mutations in ribosomal proteins on the accuracy of the two-step selection of aminoacyl-tRNAs in protein biosynthesis. , 1981, The Journal of biological chemistry.

[18]  D. Nathans,et al.  Inhibition of aminoacyl-sRNA binding to ribosomes by tetracycline , 1965 .

[19]  W. Szer,et al.  Effect of edeine on aminoacyl-tRNA binding to ribosomes and its relationship to ribosomal binding sites. , 1970, Biochimica et biophysica acta.

[20]  I. Holland,et al.  Effect of colicin E3 upon the 30S ribosomal subunit of Escherichia coli. , 1971, Proceedings of the National Academy of Sciences of the United States of America.

[21]  Ernest Frederick Gale,et al.  The Molecular basis of antibiotic action , 1972 .

[22]  C. Kurland,et al.  Altered Ribosomal Protein in Streptomycin-Dependent Escherichia coli , 1969, Science.

[23]  R. Gutell,et al.  Detailed analysis of the higher-order structure of 16S-like ribosomal ribonucleic acids. , 1983, Microbiological reviews.

[24]  H. Noller.  Structure of ribosomal RNA. , 1984, Annual review of biochemistry.

[25]  H. Noller,et al.  Localization of the binding site for protein S4 on 16 S ribosomal RNA by chemical and enzymatic probing and primer extension. , 1986, Journal of molecular biology.

[26]  M. Nomura,et al.  Identification and Functional Characterization of the Protein controlled by the Streptomycin-resistant Locus in E. coli , 1969, Nature.

[27]  E. Cundliffe,et al.  Bacterial-protein synthesis. A novel system for studying antibiotic action in vivo. , 1973, European journal of biochemistry.

[28]  N. Martin,et al.  Identification of the paromomycin-resistance mutation in the 15 S rRNA gene of yeast mitochondria. , 1982, The Journal of biological chemistry.

[29]  J. Modolell,et al.  Dual interference of hygromycin B with ribosomal translocation and with aminoacyl-tRNA recognition. , 1978, European journal of biochemistry.

[30]  J. Ofengand,et al.  Identification of the site of cross-linking in 16S rRNA of an aromatic azide photoaffinity probe attached to the 5'-anticodon base of A site bound tRNA. , 1985, Biochemistry.

[31]  P. Schürmann,et al.  Streptomycin-resistance of Euglena gracilis chloroplasts: identification of a point mutation in the 16S rRNA gene in an invariant position. , 1985, Nucleic acids research.

[32]  A. Böck,et al.  Comparative analysis of the effect of aminoglycosides on bacterial protein synthesis in vitro. , 1979, European journal of biochemistry.

[33]  M. Hierowski.  INHIBITION OF PROTEIN SYNTHESIS BY CHLORTETRACYCLINE IN THE E. COLI IN VITRO SYSTEM. , 1965, Proceedings of the National Academy of Sciences of the United States of America.

[34]  M. Cousin,et al.  Paromomycin and dihydrostreptomycin binding to Escherichia coli ribosomes. , 1976, European journal of biochemistry.

[35]  R. Gutell,et al.  Comparative anatomy of 16-S-like ribosomal RNA. , 1985, Progress in nucleic acid research and molecular biology.

[36]  J. Modolell,et al.  Functional interaction of neomycin B and related antibiotics with 30S and 50S ribosomal subunits. , 1979, Biochemical and biophysical research communications.

[37]  Harry F. Noller,et al.  Transfer RNA shields specific nucleotides in 16S ribosomal RNA from attack by chemical probes , 1986, Cell.

[38]  D. Biswas,et al.  Restriction, de-restriction and mistranslation in missense suppression. Ribosomal discrimination of transfer RNA's. , 1972, Journal of molecular biology.