Green Synthesis of Zinc Oxide Nanoparticles Using Nostoc sp. and Their Multiple Biomedical Properties

Zinc oxide nanoparticles (ZnONPs) are the top candidate in the field of biological applications because of their high surface area and excellent catalytic activities. In the present study, the cyanobacteria-mediated biosynthesis of zinc oxide NPs using Nostoc sp. extract as a stabilizing, chelating, and reducing agent is reported. ZnONPs were biologically synthesized using an eco-friendly and simple technique with a minimal reaction time and calcination temperature. Various methods, including X-ray diffraction (XRD), ultraviolet spectroscopy (UV), Fourier transform infrared (FTIR), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX) were used to characterize the biosynthesized zinc oxide NPs. XRD analysis depicted the crystalline form of zinc oxide NPs, and the Scherrer equation determined a mean crystalline size of ~28.21 nm. The SEM results reveal the spherical shape of the biosynthesized nanoparticles. Various functional groups were involved in the capping and stabilization of the zinc oxide NPs, which were confirmed by FTIR analysis. The zinc oxide NPs showed strong UV-vis absorption at 340 nm. Multiple in vitro biological applications showed significant therapeutic potential for zinc oxide NPs. Potential antimicrobial assays were reported for zinc oxide NPs via the disc-diffusion method and food poisoning method, respectively. All other activities mentioned below are described with the concentration and IC50 values. Biocompatibility with human erythrocytes and macrophages (IC50: 433 µg/mL, IC50 > 323 µg/mL) and cytotoxic properties using brine shrimps (IC50: 11.15 µg/mL) and Leishmania tropics (Amastigotes IC50: 43.14 µg mL−1 and Promastigotes IC50: 14.02 µg mL−1) were determined. Enzyme inhibition assays (protein kinase and alpha amylase) were performed and showed strong potential. Free radical scavenging tests showed strong antioxidant capacities. These results indicate that zinc oxide NPs synthesized by Nostoc sp. have strong biological applications and are promising candidates for clinical development.

[1]  M. F. H. Munis,et al.  Biocontrol of Fruit Rot of Litchi chinensis Using Zinc Oxide Nanoparticles Synthesized in Azadirachta indica , 2022, Micromachines.

[2]  Mohamed Hosny,et al.  Green synthesis of bimetallic Ag/ZnO@Biohar nanocomposite for photocatalytic degradation of tetracycline, antibacterial and antioxidant activities , 2022, Scientific Reports.

[3]  Krishnendu Pal,et al.  Antioxidant and photocatalytic properties of zinc oxide nanoparticles phyto-fabricated using the aqueous leaf extract of Sida acuta , 2022, Green Processing and Synthesis.

[4]  S. Faisal,et al.  Green Synthesis of Zinc Oxide (ZnO) Nanoparticles Using Aqueous Fruit Extracts of Myristica fragrans: Their Characterizations and Biological and Environmental Applications , 2021, ACS omega.

[5]  Ehab Azab,et al.  Green Synthesis of Zinc Oxide Nanoparticles (ZnO-NPs) Using Arthrospira platensis (Class: Cyanophyceae) and Evaluation of their Biomedical Activities , 2021, Nanomaterials.

[6]  S. Hassan,et al.  Plant Growth-Promoting Endophytic Bacterial Community Inhabiting the Leaves of Pulicaria incisa (Lam.) DC Inherent to Arid Regions , 2021, Plants.

[7]  S. Hassan,et al.  An eco-friendly approach to textile and tannery wastewater treatment using maghemite nanoparticles (γ-Fe2O3-NPs) fabricated by Penicillium expansum strain (K-w) , 2020 .

[8]  Vishnu Sankar Sivasankarapillai,et al.  Green synthesis and characterization of zinc oxide nanoparticles with antibacterial and antifungal activity , 2020 .

[9]  Dan Xu,et al.  Green synthesis of zinc oxide nanoparticles using Citrus sinensis peel extract and application to strawberry preservation: A comparison study , 2020 .

[10]  J. Iqbal,et al.  Bioactivities of Geranium wallichianum Leaf Extracts Conjugated with Zinc Oxide Nanoparticles , 2019, Biomolecules.

[11]  Yuezhou Wei,et al.  Efficient removal of uranium, cadmium and mercury from aqueous solutions using grafted hydrazide-micro-magnetite chitosan derivative , 2019, Journal of Materials Science.

[12]  R. Hamouda,et al.  Synthesis and biological characterization of silver nanoparticles derived from the cyanobacterium Oscillatoria limnetica , 2019, Scientific Reports.

[13]  M. Zangeneh,et al.  Ziziphora clinopodioides Lam leaves aqueous extract mediated synthesis of zinc nanoparticles and their antibacterial, antifungal, cytotoxicity, antioxidant, and cutaneous wound healing properties under in vitro and in vivo conditions , 2019, Applied Organometallic Chemistry.

[14]  J. Iqbal,et al.  Plant-extract mediated green approach for the synthesis of ZnONPs: Characterization and evaluation of cytotoxic, antimicrobial and antioxidant potentials , 2019, Journal of Molecular Structure.

[15]  J. Iqbal,et al.  Green synthesis and characterizations of Nickel oxide nanoparticles using leaf extract of Rhamnus virgata and their potential biological applications , 2019, Applied Organometallic Chemistry.

[16]  M. Zolfaghari,et al.  A bio-inspired strategy for the synthesis of zinc oxide nanoparticles (ZnO NPs) using the cell extract of cyanobacterium Nostoc sp. EA03: from biological function to toxicity evaluation , 2019, RSC advances.

[17]  K. Ghanemi,et al.  Phycosynthesis and Enhanced Photocatalytic Activity of Zinc Oxide Nanoparticles Toward Organosulfur Pollutants , 2019, Scientific Reports.

[18]  A. Naghizadeh,et al.  Facile green synthesis of silver nanoparticles using Berberis vulgaris leaf and root aqueous extract and its antibacterial activity. , 2019, International journal of biological macromolecules.

[19]  M. Jamzad,et al.  Green synthesis of zinc oxide nanoparticles: a comparison , 2019, Green Chemistry Letters and Reviews.

[20]  O. Dakhil,et al.  GREEN SYNTHESIS AND CHARACTERIZATION OF ZINC OXIDE NANOPARTICLES FROM CLADOPHORA GLOMERATA AND ITS ANTIFUNGAL ACTIVITY AGAINST SOME FUNGAL ISOLATES , 2019 .

[21]  J. Iqbal,et al.  Role of dietary phytochemicals in modulation of miRNA expression: Natural swords combating breast cancer , 2018 .

[22]  S. Basu,et al.  Biogenic ZnO nanoparticles: a study of blueshift of optical band gap and photocatalytic degradation of reactive yellow 186 dye under direct sunlight , 2018, Green Processing and Synthesis.

[23]  B. Ahirwar,et al.  Antioxidant and anticancer activities of green synthesized silver nanoparticles using aqueous extract of tubers of Pueraria tuberosa , 2018, Artificial cells, nanomedicine, and biotechnology.

[24]  J. Roux,et al.  Uranium and europium sorption on amidoxime-functionalized magnetic chitosan micro-particles , 2018, Chemical Engineering Journal.

[25]  Y. Lim,et al.  Cytotoxic effects of zinc oxide nanoparticles on cyanobacterium Spirulina (Arthrospira) platensis , 2018, PeerJ.

[26]  Zabta Khan Shinwari,et al.  Sageretia thea (Osbeck.) modulated biosynthesis of NiO nanoparticles and their in vitro pharmacognostic, antioxidant and cytotoxic potential , 2018, Artificial cells, nanomedicine, and biotechnology.

[27]  T. Prasad,et al.  Synthesis, characterization and evaluation of antimicrobial efficacy and brine shrimp lethality assay of Alstonia scholaris stem bark extract mediated ZnONPs , 2018, Biochemistry and biophysics reports.

[28]  A. Baqi.  Determination of antioxidants in two medicinally important plants, Haloxylon griffithii and Convolvulus leioclaycinus, of Balochistan , 2018 .

[29]  Sershen,et al.  Green synthesis of silver nanoparticles from Moringa oleifera leaf extracts and its antimicrobial potential , 2018 .

[30]  M. Sundrarajan,et al.  Green synthesis and characterization of zinc oxide nanoparticle using insulin plant (Costus pictus D. Don) and investigation of its antimicrobial as well as anticancer activities , 2018 .

[31]  T. Shaheen,et al.  Green approach for one-pot synthesis of silver nanorod using cellulose nanocrystal and their cytotoxicity and antibacterial assessment. , 2018, International journal of biological macromolecules.

[32]  S. K. Chaudhuri,et al.  Biosynthesis of zinc oxide nanoparticles using leaf extract of Calotropis gigantea: characterization and its evaluation on tree seedling growth in nursery stage , 2017, Applied Nanoscience.

[33]  Diego F. Correa,et al.  Biodiversity impacts of bioenergy production: Microalgae vs. first generation biofuels , 2017 .

[34]  I. Chung,et al.  Green synthesis of copper nanoparticles using Eclipta prostrata leaves extract and their antioxidant and cytotoxic activities , 2017, Experimental and therapeutic medicine.

[35]  A. Khaledi-Nasab,et al.  Potato extract as reducing agent and stabiliser in a facile green one-step synthesis of ZnO nanoparticles , 2016 .

[36]  B. Mirza,et al.  Extraction optimization of medicinally important metabolites from Datura innoxia Mill.: an in vitro biological and phytochemical investigation , 2015, BMC Complementary and Alternative Medicine.

[37]  K. Dey,et al.  Microwave synthesis of ZnO@mSiO₂ for detailed antifungal mode of action study: understanding the insights into oxidative stress. , 2015, Journal of colloid and interface science.

[38]  B. Mahanty,et al.  Biosynthesis of hematite nanoparticles and its cytotoxic effect on HepG2 cancer cells. , 2015, International journal of biological macromolecules.

[39]  V. Rajendran,et al.  Rambutan peels promoted biomimetic synthesis of bioinspired zinc oxide nanochains for biomedical applications. , 2015, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[40]  D. Late,et al.  Antimicrobial Activity of ZnO Nanoparticles against Pathogenic Bacteria and Fungi , 2015 .

[41]  M. Arshad,et al.  Zinc Oxide Nanoparticles for Revolutionizing Agriculture: Synthesis and Applications , 2014, TheScientificWorldJournal.

[42]  L. Laânab,et al.  One step synthesis of ZnO nanoparticles in free organic medium: Structural and optical characterizations , 2014 .

[43]  M. Darroudi,et al.  Green chemistry approach for the synthesis of ZnO nanopowders and their cytotoxic effects , 2014 .

[44]  Susan Azizi,et al.  Green biosynthesis and characterization of zinc oxide nanoparticles using brown marine macroalga Sargassum muticum aqueous extract , 2014 .

[45]  D. El-Hady,et al.  Green Synthesis of Silver Nanoparticles by Water Soluble Fraction of the Extracellular Polysaccharides/Matrix of the Cyanobacterium Nostoc Commune and its Application as a Potent Fungal Surface Sterilizing Agent of Seed Crops , 2014 .

[46]  S. Nagarajan,et al.  Extracellular synthesis of zinc oxide nanoparticle using seaweeds of gulf of Mannar, India , 2013, Journal of Nanobiotechnology.

[47]  Zijiong Li,et al.  High-performance solid-state supercapacitors based on graphene-ZnO hybrid nanocomposites , 2013, Nanoscale Research Letters.

[48]  S. Rajeshwari,et al.  Bio-fabrication of zinc oxide nanoparticles using leaf extract of Parthenium hysterophorus L. and its size-dependent antifungal activity against plant fungal pathogens. , 2013, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[49]  Yusuf Chisti,et al.  Synthesis of metallic nanoparticles using plant extracts. , 2013, Biotechnology advances.

[50]  V. Stone,et al.  Zinc oxide nanoparticles and monocytes: impact of size, charge and solubility on activation status. , 2013, Toxicology and applied pharmacology.

[51]  R. Sivaraj,et al.  Green synthesized ZnO nanoparticles against bacterial and fungal pathogens , 2012 .

[52]  Hedi Mattoussi,et al.  The state of nanoparticle-based nanoscience and biotechnology: progress, promises, and challenges. , 2012, ACS nano.

[53]  Yongsheng Chen,et al.  Mechanism of photogenerated reactive oxygen species and correlation with the antibacterial properties of engineered metal-oxide nanoparticles. , 2012, ACS nano.

[54]  T. Özer,et al.  Fourier transform infrared (FTIR) spectroscopy for identification of Chlorella vulgaris Beijerinck 1890 and Scenedesmus obliquus (Turpin) Kützing 1833 , 2012 .

[55]  B. Mirza,et al.  Antioxidant and Cytotoxic Activities and Phytochemical Analysis of Euphorbia wallichii Root Extract and its Fractions , 2012, Iranian journal of pharmaceutical research : IJPR.

[56]  S. Chanda,et al.  Brine shrimp cytotoxicity of Caesalpinia pulcherrima aerial parts, antimicrobial activity and characterisation of isolated active fractions , 2011, Natural product research.

[57]  G. Tan,et al.  Citrinin derivatives from the soil filamentous fungus Penicillium sp. H9318 , 2011 .

[58]  R. Varma,et al.  Green synthesis of metal nanoparticles: Biodegradable polymers and enzymes in stabilization and surface functionalization , 2011 .

[59]  Gaoke Zhang,et al.  Facile Synthesis of Monodisperse Porous ZnO Spheres by a Soluble Starch-Assisted Method and Their Photocatalytic Activity , 2011 .

[60]  Siavash Iravani,et al.  Green synthesis of metal nanoparticles using plants , 2011 .

[61]  Peter Rodgers,et al.  Nanoscience and technology : a collection of reviews from nature journals , 2009 .

[62]  R. P. Rastogi,et al.  Biotechnological and industrial significance of cyanobacterial secondary metabolites. , 2009, Biotechnology advances.

[63]  Qin Guo,et al.  Recent Advances in Nanotechnology Applied to Biosensors , 2009, Sensors.

[64]  Rajender S. Varma,et al.  Green synthesis of silver and palladium nanoparticles at room temperature using coffee and tea extract , 2008 .

[65]  Anil K Patri,et al.  Method for analysis of nanoparticle hemolytic properties in vitro. , 2008, Nano letters.

[66]  Mritunjai Singh,et al.  NANOTECHNOLOGY IN MEDICINE AND ANTIBACTERIAL EFFECT OF SILVER NANOPARTICLES , 2008 .

[67]  D. Malagoli.  A full-length protocol to test hemolytic activity of palytoxin on human erythrocytes , 2007 .

[68]  Ie-Hong Hong,et al.  Diameter dependence of the electronic structure of ZnO nanorods determined by x-ray absorption spectroscopy and scanning photoelectron microscopy , 2004 .

[69]  Jianrong Chen,et al.  Nanotechnology and biosensors. , 2004, Biotechnology advances.

[70]  M. Edlund.  Freshwater Algae of North America. Ecology and Classification , 2003 .

[71]  J. Davies,et al.  Identifying protein kinase inhibitors using an assay based on inhibition of aerial hyphae formation in Streptomyces. , 2002, The Journal of antibiotics.

[72]  A. Barral,et al.  A simple method for human peripheral blood monocyte isolation. , 2000, Memorias do Instituto Oswaldo Cruz.