Positive regulatory interactions of the HIS4 gene of Saccharomyces cerevisiae

The role of cis- and trans-acting elements in the expression of HIS4 has been examined by using HIS4-lacZ fusions in which lacZ expression is dependent upon the HIS4 5' noncoding region. The cis-acting sequences involved in regulation were defined by studying the effects of the wild-type and various deletions and their revertants on regulation via the general control of amino acid biosynthesis. The role of trans-acting genes was analyzed by studying the regulation of the HIS4-lacZ fusions in strains carrying mutations in the GCN (AAS) or GCD (TRA) genes and in strains carrying the GCN genes on high-copy-number plasmids. These studies have led to the following conclusions. (i) HIS4 is positively regulated by the general control. (ii) At least one copy of the 5'TGACTC3' repeat at -136 is required in cis for this regulation. (iii) Both the GCN4 gene and at least one copy of the repeated sequence are required for expression at the repressed level. (iv) The open reading frames in the 5' noncoding region are not required in either cis or trans for the regulation of HIS4.

[1]  Jeffrey H. Miller.  Experiments in molecular genetics , 1972 .

[2]  R. Hütter,et al.  Regulation of Tryptophan Biosynthesis in Saccharomyces cerevisiae: Mode of Action of 5-Methyl-Tryptophan and 5-Methyl-Tryptophan-Sensitive Mutants , 1974, Journal of bacteriology.

[3]  F. Messenguy,et al.  The regulation of arginine biosynthesis in Saccharomyces cerevisiae. The specificity of argR- mutations and the general control of amino-acid biosynthesis. , 1975, European journal of biochemistry.

[4]  F. Messenguy,et al.  Integration of amino acid biosynthesis into the cell cycle of Saccharomyces cerevisiae. , 1975, Journal of molecular biology.

[5]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[6]  P. Niederberger,et al.  Tryptophan biosynthesis in Saccharomyces cerevisiae: control of the flux through the pathway , 1978, Journal of bacteriology.

[7]  J. Broach,et al.  TRANSFORMATION IN YEAST: DEVELOPMENT OF A HYBRID CLON- ING VECTOR AND ISOLATION OF THE CAN! GENE (Saccharomyces cerevisiae, plasmid vector; arginine permease) , 1979 .

[8]  G. Fink,et al.  Methods in yeast genetics , 1979 .

[9]  D. Botstein,et al.  Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. , 1979, Gene.

[10]  R. W. Davis,et al.  A physical, genetic and transcriptional map of the cloned his3 gene region of Saccharomyces cerevisiae. , 1980, Journal of molecular biology.

[11]  D. Botstein,et al.  Yeast genes fused to beta-galactosidase in Escherichia coli can be expressed normally in yeast. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[12]  Ralf,et al.  Biological role of the general control of amino acid biosynthesis in Saccharomyces cerevisiae , 1981, Molecular and cellular biology.

[13]  G. Fink,et al.  The nucleotide sequence of the HIS4 region of yeast. , 1982, Gene.

[14]  Regulation of HIS4-lacZ fusions in Saccharomyces cerevisiae. , 1982, Molecular and cellular biology.

[15]  K. Struhl.  Regulatory sites for his3 gene expression in yeast , 1982, Nature.

[16]  C. Yanofsky,et al.  Yeast gene TRP5: structure, function, regulation. , 1982, The Journal of biological chemistry.

[17]  S. Johnston,et al.  Isolation of the yeast regulatory gene GAL4 and analysis of its dosage effects on the galactose/melibiose regulon. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[18]  G. Fink,et al.  Regulation of Amino Acid and Nucleotide Biosynthesis in Yeast , 1982 .

[19]  G. Fink,et al.  Repeated DNA sequences upstream from HIS1 also occur at several other co-regulated genes in Saccharomyces cerevisiae. , 1983, The Journal of biological chemistry.

[20]  G. Lucchini,et al.  A short nucleotide sequence required for regulation of HIS4 by the general control system of yeast , 1983, Cell.

[21]  M. Penn,et al.  Identification of AAS genes and their regulatory role in general control of amino acid biosynthesis in yeast. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[22]  G. Fink,et al.  Positive regulation in the general amino acid control of Saccharomyces cerevisiae. , 1983, Proceedings of the National Academy of Sciences of the United States of America.