The invention belongs to the technical field of road design, and relates to a method for determining the minimum distance of road intersections in a harbor district. In the method, frequent lane-changing area of harbor district roads connected with the intersections and the intersections are subjected to two-dimensional cell division, and other parts are one-dimensional cells, and a cell model for harbor district roads near the intersections is constructed. The method comprises the following steps of: (1) constructing a driver sensing module; (2) constructing a behavior module; and (3) setting appearing ratios of various vehicles in a main road, speeds of the various vehicles, and departure frequencies of various vehicles, performing simulation experiments under the conditions of different distances of the intersections and different departure frequencies, and analyzing the simulation to obtain a traffic flow space-time map and vehicle delay data so as to obtain the minimum distance of proper intersections under different traffic conditions. By the method, the influence of the distance of the intersections on the road capacity of the harbor district can be accurately reflected, sothat a method for determining the distance of the road intersections in the harbor district under different conditions is provided.