Decolorization of an azo dye by unacclimated activated sludge under anaerobic conditions

Abstract Studies on the reductive decolorization of a complex azo dye, Reactive Red 3.1, were made as part of the development of a practical approach to better exploit the metabolic potential of biomass in wastewater treatment. Decolorization was achieved at low and variable rates by mixed microbial cultures under various environmental conditions, including low pH and high salt concentration. It was caused by reductive cleavage of the azo bond to yield two aromatic amines. More reliable and effective decolorization rates, of up to 20–30 mg l−1 h−1, were given by unadapted activated sludge, (6 g l−1) incubated with 400 mg l−1 of Reactive Red 3.1 under anaerobic conditions. Decolorization also occurred best in static conditions.

[1]  I. Banat,et al.  Microbial process for the decolorization of textile effluent containing azo, diazo and reactive dyes , 1996 .

[2]  T. Ohe,et al.  Microbial degradation of 1, 6-and 2, 6-naphthalenedisulfonic acid by Pseudomonas sp. DS-1 , 1988 .

[3]  J. Knapp,et al.  The microbiological decolorization of an industrial effluent containing a diazo-linked chromophore , 1995 .

[4]  H. D. Stensel,et al.  Anaerobic and aerobic treatment of chlorinated aliphatic compounds , 1993 .

[5]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[6]  P. Bishop,et al.  Two stage anaerobic/aerobic treatment of sulfonated azo dyes , 1995 .

[7]  H. Knackmuss,et al.  Degradation of naphthalene-2,6- and naphthalene-1,6-disulfonic acid by a Moraxella sp , 1988, Applied and environmental microbiology.

[8]  T. Thurnheer,et al.  Orthanilic Acid and Analogues as Carbon Sources for Bacteria: Growth Physiology and Enzymic Desulphonation , 1986 .

[9]  H. Knackmuss,et al.  Bacterial communities degrading amino- and hydroxynaphthalene-2-sulfonates , 1986, Applied and environmental microbiology.

[10]  T. Ohe,et al.  Degradation of 2-Naphthylamine-l-sulfonic Acid by Pseudomonas Strain TA-1 , 1986 .

[11]  Paul L. Bishop,et al.  Anaerobic/aerobic treatment of selected azo dyes in wastewater , 1994 .

[12]  D. Hempel,et al.  Mineralization of the sulfonated azo dye Mordant Yellow 3 by a 6-aminonaphthalene-2-sulfonate-degrading bacterial consortium , 1991, Applied and environmental microbiology.

[13]  A. Stolz,et al.  Autoxidation Reactions of Different Aromatic o-Aminohydroxynaphthalenes That Are Formed during the Anaerobic Reduction of Sulfonated Azo Dyes , 1999 .

[14]  E. Carliell,et al.  Microbial decolourisation of a reactive azo dye under anaerobic conditions , 1995 .

[15]  G. Lettinga,et al.  Detoxification and partial mineralization of the azo dye mordant orange 1 in a continuous upflow anaerobic sludge-blanket reactor , 1997, Applied Microbiology and Biotechnology.

[16]  Mark A. Brown,et al.  Predicting azo dye toxicity , 1993 .

[17]  G. Wedemeyer.  Dechlorination of 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane by Aerobacter aerogenes. I. Metabolic products. , 1967, Applied microbiology.