论文信息 - Identification and antigenic comparison of equine arteritis virus isolated from an outbreak of epidemic abortion of mares.

Identification and antigenic comparison of equine arteritis virus isolated from an outbreak of epidemic abortion of mares.

Summary Five aborted foetuses and a foal from a half-breed English horse stud (P) in which the abortion rate reached 40 % were studied. After four to eight passages of liver, spleen and lung homogenates in rabbit kidney (RK-13) and monkey kidney (Vero) cell cultures, six cytopathic agents were isolated. They were identified as Equine Arteritis Virus (EAV) using the fluorescent antibody test. One of the new isolates (Wroclaw-2) which was studied by the cross-virus neutralization test in comparison to the Bucyrus strain of EAV showed antigenic identity with the prototype virus. Zusammenfassung Identifizierung und antigenetische Untersuchung von Equinem Arteritis Virus, das bei einem Ausbruch von Stutenabort isoliert wurde In einem Halbblutgerstut lag die Abortrate bei 40 %. Leber, Lunge und Milz von funf Foeten und einem Fohlen wurden virologisch untersucht. Nach 4 bis 8 Passagen des homogenisierten Gewebes auf Kaninchennieren- (RK-13) bzw. Affennierenzellkulturen (Vero) konnte in allen sechs Fallen ein cytopathisches Agens isoliert werden. Mit Hilfe der Immunfluoreszenz wurde es als Equines Arteritis Virus (EAV) identifiziert. Eines der neuen Isolate (Wroclaw-2) wurde im Kreuzneutralisationstest mit dem EAV-Referenzstamm Bucyrus verglichen. Dabei bestatigte sich die antigenetische Identitat der beiden Viren.

A. Moraillon | W. Golnik | J. Golnik

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