A nanoceria-platinum-graphene nanocomposite for electrochemical biosensing.

Most graphene-metal nanocomposites for biosensing are formed using noble metals. Recently, development of nanocomposites using rare earth metals has gained much attention. This paper reports on the development of a nanoceria-nanoplatinum-graphene hybrid nanocomposite as a base transducing layer for mediator-free enzymatic biosensors. The hybrid nanocomposite was shown to improve detection of superoxide or hydrogen peroxide when compared to other carbon-metal hybrid nanocomposites. Based on this finding, the nanocomposite was applied for biosensing by adding either a peroxide-producing oxidase (glucose oxidase), or a superoxide-producing oxidase (xanthine oxidase). Material analysis indicated that nanoceria and nanoplatinum were equally distributed along the surface of the hybrid material, ensuring detection of either superoxide or hydrogen peroxide produced by oxidase activity. Glucose biosensors demonstrated a sensitivity (66.2±2.6μAmM(-1)cm(-2)), response time (6.3±3.4s), and limit of detection (1.3±0.6μM) that were comparable to other graphene-mediated electrodes in the current literature. Remarkably, XOD biosensor sensitivity (1164±332μAmM(-1)), response time (5.0±1.5s), and limit of detection (0.2±0.1μM) were higher than any reported biosensors using similar metal-decorated carbon nanomaterials. This material is the first demonstration of a highly efficient, diverse nanoceria/nanoplatinum/graphene hybrid nanocomposite for biosensing.

[1]  S. Stankovich,et al.  Graphene-based composite materials , 2006, Nature.

[2]  Bingbing Liu,et al.  Controlled Synthesis of CeO2/Graphene Nanocomposites with Highly Enhanced Optical and Catalytic Properties , 2012 .

[3]  Meihe Zhang,et al.  Cerium oxide-graphene as the matrix for cholesterol sensor. , 2013, Analytical biochemistry.

[4]  Fan Zhang,et al.  Photoluminescence modification in upconversion rare-earth fluoride nanocrystal array constructed photonic crystals , 2010 .

[5]  Jin Zhai,et al.  Hierarchically ordered macro-mesoporous TiO₂-graphene composite films: improved mass transfer, reduced charge recombination, and their enhanced photocatalytic activities. , 2011, ACS nano.

[6]  Y. Tong,et al.  Redox cycles promoting photocatalytic hydrogen evolution of CeO2 nanorods , 2011 .

[7]  Jian Fang,et al.  Amperometric detection of hydrogen peroxide utilizing synergistic action of cobalt hexacyanoferrate and carbon nanotubes chemically modified with platinum nanoparticles , 2013 .

[8]  Hua Zhang,et al.  Graphene-based composites. , 2012, Chemical Society reviews.

[9]  Jing Ning,et al.  Reduced Graphene Oxide‐Mediated Growth of Uniform Tin‐Core/Carbon‐Sheath Coaxial Nanocables with Enhanced Lithium Ion Storage Properties , 2012, Advanced materials.

[10]  H. Shin,et al.  Reduced graphene oxide (rGO)-wrapped fullerene (C₆₀) wires. , 2011, ACS nano.

[11]  J. Ahn,et al.  Multi-walled carbon nanotubes for the immobilization of enzyme in glucose biosensors , 2003 .

[12]  Xiaoping Shen,et al.  Synthesis of reduced graphene oxide/CeO2 nanocomposites and their photocatalytic properties , 2013, Nanotechnology.

[13]  C. L. Cheung,et al.  Defect engineering in cubic cerium oxide nanostructures for catalytic oxidation. , 2011, Nano letters.

[14]  Tom Regier,et al.  Co₃O₄ nanocrystals on graphene as a synergistic catalyst for oxygen reduction reaction. , 2011, Nature materials.

[15]  J. Luong,et al.  Electrochemical biosensing platforms using platinum nanoparticles and carbon nanotubes. , 2004, Analytical chemistry.

[16]  K. N. Sood,et al.  Nanostructured cerium oxide film for triglyceride sensor , 2009 .

[17]  Sandeep Yadav,et al.  Amperometric determination of xanthine in fish meat by zinc oxide nanoparticle/chitosan/multiwalled carbon nanotube/polyaniline composite film bound xanthine oxidase. , 2012, The Analyst.

[18]  Eric S McLamore,et al.  A comparative study of carbon-platinum hybrid nanostructure architecture for amperometric biosensing. , 2014, The Analyst.

[19]  A. Karakoti,et al.  Enzyme-free detection of hydrogen peroxide from cerium oxide nanoparticles immobilized on poly(4-vinylpyridine) self-assembled monolayers. , 2013, Journal of materials chemistry. B.

[20]  Qing Peng,et al.  Enhanced catalytic activity of ceria nanorods from well-defined reactive crystal planes , 2005 .

[21]  C. R. Raj,et al.  Development of an Amperometric Cholesterol Biosensor Based on Graphene-Pt Nanoparticle Hybrid Material , 2010 .

[22]  Benjamin Gilbert,et al.  Comparison of the mechanism of toxicity of zinc oxide and cerium oxide nanoparticles based on dissolution and oxidative stress properties. , 2008, ACS nano.

[23]  Dingcai Wu,et al.  Reactive template-induced self-assembly to ordered mesoporous polymeric and carbonaceous materials. , 2013, ACS nano.

[24]  J. Rayappan,et al.  Lipase immobilized on nanostructured cerium oxide thin film coated on transparent conducting oxide electrode for butyrin sensing , 2013 .

[25]  Xiaoping Shen,et al.  Graphene–inorganic nanocomposites , 2012 .

[26]  Andre K. Geim,et al.  The rise of graphene. , 2007, Nature materials.

[27]  Yeliang Wang,et al.  Intercalation of metal islands and films at the interface of epitaxially grown graphene and Ru(0001) surfaces , 2011 .

[28]  Huimin Zhang,et al.  3D porous and redox-active prussian blue-in-graphene aerogels for highly efficient electrochemical detection of H2O2 , 2012 .

[29]  R. Villalonga,et al.  Supramolecular immobilization of xanthine oxidase on electropolymerized matrix of functionalized hybrid gold nanoparticles/single-walled carbon nanotubes for the preparation of electrochemical biosensors. , 2012, ACS applied materials & interfaces.

[30]  B. Rezaei,et al.  A novel enzyme-free amperometric sensor for hydrogen peroxide based on Nafion/exfoliated graphene oxide-Co3O4 nanocomposite. , 2013, Talanta.

[31]  Dongyang Li,et al.  Mechanical properties and erosion resistance of ceria nano-particle-doped ultrafine WC–12Co composite prepared by spark plasma sintering , 2013 .

[32]  S. Ramaprabhu,et al.  Facile and simultaneous production of metal/metal oxide dispersed graphene nano composites by solar exfoliation , 2011 .

[33]  Tymish Y. Ohulchanskyy,et al.  High contrast in vitro and in vivo photoluminescence bioimaging using near infrared to near infrared up-conversion in Tm3+ and Yb3+ doped fluoride nanophosphors. , 2008, Nano letters.

[34]  Juan Tang,et al.  In situ amplified electronic signal for determination of low-abundance proteins coupling with nanocatalyst-based redox cycling. , 2013, Chemical communications.

[35]  Changwen Hu,et al.  Review on the latest design of graphene-based inorganic materials. , 2012, Nanoscale.

[36]  B. D. Malhotra,et al.  Quantum dots based platform for application to fish freshness biosensor , 2013 .

[37]  J SmithDavid,et al.  超高真空透過電子顕微鏡法を用いたSi(110)上におけるCoSi2ナノワイヤーのエンドタキシャル成長のその場観測 , 2011 .

[38]  Bei Wang,et al.  FACILE SYNTHESIS AND CHARACTERIZATION OF GRAPHENE NANOSHEETS , 2008 .

[39]  C. Liu,et al.  Preparation and characterization of three dimensional graphene foam supported platinum-ruthenium bimetallic nanocatalysts for hydrogen peroxide based electrochemical biosensors. , 2014, Biosensors & bioelectronics.

[40]  D Marshall Porterfield,et al.  A comparative study of enzyme immobilization strategies for multi-walled carbon nanotube glucose biosensors , 2011, Nanotechnology.

[41]  Kwang S. Kim,et al.  Large-scale pattern growth of graphene films for stretchable transparent electrodes , 2009, Nature.

[42]  Jian Xie,et al.  An aqueous media based approach for the preparation of a biosensor platform composed of graphene oxide and Pt-black. , 2012, Biosensors & bioelectronics.

[43]  Eric S. McLamore,et al.  Electrochemical glutamate biosensing with nanocube and nanosphere augmented single-walled carbon nanotube networks: a comparative study , 2011 .

[44]  G. Adachi,et al.  Rare earth contribution in solid state electrolytes, especially in the chemical sensor field , 1997 .

[45]  S. Khondaker,et al.  Anchoring ceria nanoparticles on reduced graphene oxide and their electronic transport properties , 2011, 1107.1745.

[46]  G. Fudenberg,et al.  Ultrahigh electron mobility in suspended graphene , 2008, 0802.2389.

[47]  Bansi D. Malhotra,et al.  Sol-gel derived nanostructured cerium oxide film for glucose sensor , 2008 .

[48]  Guy Denuault,et al.  Detection of hydrogen peroxide at mesoporous platinum microelectrodes. , 2002, Analytical chemistry.

[49]  S. Stankovich,et al.  Synthesis of graphene-based nanosheets via chemical reduction of exfoliated graphite oxide , 2007 .

[50]  Feng Li,et al.  Anchoring Hydrous RuO2 on Graphene Sheets for High‐Performance Electrochemical Capacitors , 2010 .

[51]  O. Akhavan.  Photocatalytic reduction of graphene oxides hybridized by ZnO nanoparticles in ethanol , 2011 .

[52]  R. Ruoff,et al.  Graphene and Graphene Oxide: Synthesis, Properties, and Applications , 2010, Advanced materials.

[53]  G. Wallace,et al.  Processable aqueous dispersions of graphene nanosheets. , 2008, Nature nanotechnology.

[54]  Roberto Car,et al.  Functionalized single graphene sheets derived from splitting graphite oxide. , 2006, The journal of physical chemistry. B.

[55]  Jae-Joon Lee,et al.  A Comprehensive Review of Glucose Biosensors Based on Nanostructured Metal-Oxides , 2010, Sensors.

[56]  E S McLamore,et al.  A self referencing platinum nanoparticle decorated enzyme-based microbiosensor for real time measurement of physiological glucose transport. , 2011, Biosensors & bioelectronics.

[57]  Gang Wang,et al.  Prepartion and electrochemical performance of a cerium oxide-graphene nanocomposite as the anode material of a lithium ion battery , 2011 .

[58]  H. Ju,et al.  Amperometric detection of hypoxanthine and xanthine by enzymatic amplification using a gold nanoparticles-carbon nanohorn hybrid as the carrier. , 2012, The Analyst.

[59]  B. Habibi,et al.  Voltammetric and amperometric determination of hydrogen peroxide using a carbon-ceramic electrode modified with a nanohybrid composite made from single-walled carbon nanotubes and silver nanoparticles , 2012, Microchimica Acta.

[60]  T. Chung,et al.  Synthesis of a graphene–carbon nanotube composite and its electrochemical sensing of hydrogen peroxide , 2012 .

[61]  A. Govindaraj,et al.  Graphene: the new two-dimensional nanomaterial. , 2009, Angewandte Chemie.

[62]  SUPARNA DUTTASINHA,et al.  Graphene: Status and Prospects , 2009, Science.

[63]  Xiaohong Sun,et al.  Fabrication of Ag@SiO(2)@Y(2)O(3):Er nanostructures for bioimaging: tuning of the upconversion fluorescence with silver nanoparticles. , 2010, Journal of the American Chemical Society.

[64]  Ping Li,et al.  A high-sensitive and fast-fabricated glucose biosensor based on Prussian blue/topological insulator Bi2Se3 hybrid film. , 2012, Biosensors & bioelectronics.

[65]  Bingbing Liu,et al.  Shape-selective synthesis and optical performance of ceria nanocrystal/graphene hybrid composites , 2013 .

[66]  H. Cho,et al.  A novel multivalent nanomaterial based hydrogen peroxide sensor , 2007 .

[67]  P. Solanki,et al.  Sol–gel derived nanoporous cerium oxide film for application to cholesterol biosensor , 2008 .

[68]  Huangxian Ju,et al.  Amperometric glucose sensor based on catalytic reduction of dissolved oxygen at soluble carbon nanofiber. , 2007, Biosensors & bioelectronics.

[69]  Yueming Li,et al.  P25-graphene composite as a high performance photocatalyst. , 2010, ACS nano.

[70]  S P Singh,et al.  Nanoporous cerium oxide thin film for glucose biosensor. , 2009, Biosensors & bioelectronics.

[71]  Feng Li,et al.  High-energy MnO2 nanowire/graphene and graphene asymmetric electrochemical capacitors. , 2010, ACS nano.