Strain ATCC 4720T is the authentic type strain of Agrobacterium tumefaciens, which is not a later heterotypic synonym of Agrobacterium radiobacter.

The original type strains of Agrobacterium radiobacter and Agrobacterium tumefaciens recorded in the eighth edition of Bergey's Manual of Determinative Bacteriology published in 1974 were NCIB 9042T and ATCC 4720T, respectively. However, in the list of the valid names of bacteria compiled in 1980, both strains were changed, A. radiobacter NCIB 9042T to ATCC 19358T and A. tumefaciens ATCC 4720T to ATCC 23308T. These changes were unjustified, particularly in the case of A. tumefaciens whose type strain was replaced by another strain from the same collection, although the original type strain ATCC 4720T was never lost and it is currently available in several culture collections. Therefore, we request that the type strain of A. tumefaciens be corrected from ATCC 23308T to ATCC 4720T.

[1]  M. Hungria,et al.  Genetic diversity of Agrobacterium species isolated from nodules of common bean and soybean in Brazil, Mexico, Ecuador and Mozambique, and description of the new species Agrobacterium fabacearum sp. nov. , 2020, International journal of systematic and evolutionary microbiology.

[2]  E. Velázquez,et al.  Identification of Species and Subspecies of Lactic Acid Bacteria Present in Spanish Cheeses Type “Torta” by MALDI-TOF MS and pheS gene Analyses , 2020, Microorganisms.

[3]  Sudhir Kumar,et al.  MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. , 2016, Molecular biology and evolution.

[4]  A. Willems,et al.  Revised phylogeny of Rhizobiaceae: proposal of the delineation of Pararhizobium gen. nov., and 13 new species combinations. , 2015, Systematic and applied microbiology.

[5]  X. Nesme,et al.  Rapid and Efficient Identification of Agrobacterium Species by recA Allele Analysis , 2010, Microbial Ecology.

[6]  P. García-Fraile,et al.  Characterization of xylanolytic bacteria present in the bract phyllosphere of the date palm Phoenix dactylifera , 2007, Letters in applied microbiology.

[7]  J. Thioulouse,et al.  Identification of Genomic Species in Agrobacterium Biovar 1 by AFLP Genomic Markers , 2006, Applied and Environmental Microbiology.

[8]  G. Perrière,et al.  A mathematical method for determining genome divergence and species delineation using AFLP. , 2002, International journal of systematic and evolutionary microbiology.

[9]  J. Young,et al.  Phylogenies of atpD and recA support the small subunit rRNA-based classification of rhizobia. , 2001, International journal of systematic and evolutionary microbiology.

[10]  J. S. Rogers,et al.  A fast method for approximating maximum likelihoods of phylogenetic trees from nucleotide sequences. , 1998, Systematic biology.

[11]  J. Thompson,et al.  The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. , 1997, Nucleic acids research.

[12]  H. Sawada,et al.  Proposal for rejection of Agrobacterium tumefaciens and revised descriptions for the genus Agrobacterium and for Agrobacterium radiobacter and Agrobacterium rhizogenes. , 1993, International journal of systematic bacteriology.

[13]  M. Kimura A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences , 1980, Journal of Molecular Evolution.

[14]  J. Chun,et al.  Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. , 2018, International journal of systematic and evolutionary microbiology.

[15]  P. Sneath,et al.  Approved lists of bacterial names. , 1980, The Medical journal of Australia.