How to Catch a Smurf? - Ageing and Beyond… In vivo Assessment of Intestinal Permeability in Multiple Model Organisms.

The Smurf Assay (SA) was initially developed in the model organism Drosophila melanogaster where a dramatic increase of intestinal permeability has been shown to occur during aging (Rera et al., 2011). We have since validated the protocol in multiple other model organisms (Dambroise et al., 2016) and have utilized the assay to further our understanding of aging (Tricoire and Rera, 2015; Rera et al., 2018). The SA has now also been used by other labs to assess intestinal barrier permeability (Clark et al., 2015; Katzenberger et al., 2015; Barekat et al., 2016; Chakrabarti et al., 2016; Gelino et al., 2016). The SA in itself is simple; however, numerous small details can have a considerable impact on its experimental validity and subsequent interpretation. Here, we provide a detailed update on the SA technique and explain how to catch a Smurf while avoiding the most common experimental fallacies.

[1]  C. Vallot,et al.  The Smurf transition: new insights on ageing from end-of-life studies in animal models , 2018, Current opinion in oncology.

[2]  S. Panowski,et al.  Intestinal Autophagy Improves Healthspan and Longevity in C. elegans during Dietary Restriction , 2016, PLoS genetics.

[3]  M. Lipinski,et al.  Using Drosophila as an integrated model to study mild repetitive traumatic brain injury , 2016, Scientific Reports.

[4]  B. Lemaître,et al.  Remote Control of Intestinal Stem Cell Activity by Haemocytes in Drosophila , 2016, PLoS genetics.

[5]  L. Partridge,et al.  Sex difference in pathology of the ageing gut mediates the greater response of female lifespan to dietary restriction , 2016, eLife.

[6]  H. Aguilaniu,et al.  Two phases of aging separated by the Smurf transition as a public path to death , 2015, Scientific Reports.

[7]  M. Pellegrini,et al.  Distinct Shifts in Microbiota Composition during Drosophila Aging Impair Intestinal Function and Drive Mortality. , 2015, Cell reports.

[8]  H. Tricoire,et al.  Modelizing Drosophila melanogaster longevity curves using a new discontinuous 2-Phases of Aging model , 2015, bioRxiv.

[9]  D. Wassarman,et al.  Death following traumatic brain injury in Drosophila is associated with intestinal barrier dysfunction , 2015, eLife.

[10]  Chereen Collymore,et al.  Gavaging adult zebrafish. , 2013, Journal of visualized experiments : JoVE.

[11]  D. Walker,et al.  Intestinal barrier dysfunction links metabolic and inflammatory markers of aging to death in Drosophila , 2012, Proceedings of the National Academy of Sciences.

[12]  D. Walker,et al.  Modulation of longevity and tissue homeostasis by the Drosophila PGC-1 homolog. , 2011, Cell metabolism.

[13]  Linda Partridge,et al.  Quantification of Food Intake in Drosophila , 2009, PloS one.

[14]  Hao Lu,et al.  Direct Data Manipulation for Local Decision Analysis as Applied to the Problem of Arsenic in Drinking Water from Tube Wells in Bangladesh , 2004, Risk analysis : an official publication of the Society for Risk Analysis.

[15]  Carey,et al.  Below‐threshold mortality: implications for studies in evolution, ecology and demography , 1999 .