Variation in Fusarium graminearum isolates from Nepal associated with their host of origin

A collection of group II Fusarium graminearum isolates obtained from maize, wheat and rice from different locations in Nepal were identified using a combination of morphological and molecular criteria. The variation within this collection was analysed using RAPD markers, intergenic spacer (IGS) RFLP and PCR polymorphisms. The isolates were divided into two groups, A and B, by RAPD analysis. Isolates in group A yielded four different PCR polymorphic markers, but all the isolates in group B yielded a single polymorphic marker. The IGS RFLP analysis was consistent with division of the isolates into two groups. Isolates from wheat and rice were more frequently placed in group A, with isolates from maize more evenly distributed between the groups. Results indicate that host preference might be a factor in the division of isolates, although the year of isolation may also have had an influence. No geographical factors or agricultural practices could be identified to account for the observed variation.

[1]  C. Maragos,et al.  Occurrence of Fusarium species and mycotoxins in nepalese maize and wheat and the effect of traditional processing methods on mycotoxin levels. , 2000, Journal of agricultural and food chemistry.

[2]  F. Workneh,et al.  Molecular Identification of Fungi Associated with Vascular Discoloration of Soybean in the North Central United States. , 2000, Plant disease.

[3]  R. Hamelin,et al.  Genetic diversity among Fusarium graminearum strains from Ontario and Quebec , 1999 .

[4]  A. Osbourn,et al.  Isolation, Characterization, and Avenacin Sensitivity of a Diverse Collection of Cereal-Root-Colonizing Fungi , 1999, Applied and Environmental Microbiology.

[5]  K. O’Donnell,et al.  Morphological and molecular characterization of Fusarium pseudograminearum sp. nov., formerly recognized as the Group 1 population of F. graminearum , 1999 .

[6]  J. Percich,et al.  Fusarium Head Blight of Cultivated and Natural Wild Rice (Zizania palustris) in Minnesota Caused by Fusarium graminearum and Associated Fusarium spp. , 1999, Plant disease.

[7]  J. Leslie,et al.  Sexual Recombination in Gibberella zeae. , 1999, Phytopathology.

[8]  D. Joyce,et al.  DETECTION AND QUANTIFICATION OF FUSARIUM CULMORUM AND FUSARIUM GRAMINEARUM IN CEREALS USING PCR ASSAYS , 1998 .

[9]  K. O’Donnell,et al.  Molecular systematics and phylogeography of Gibberella fujikuroi species complex , 1998 .

[10]  J. Miller,et al.  Effect of tillage practice on Fusarium head blight of wheat. , 1998 .

[11]  D. Joyce,et al.  Differentiation and quantification of the cereal eyespot fungi Tapesia yallundae and Tapesia acuformis using a PCR assay. , 1997 .

[12]  T. Miedaner,et al.  Molecular Variation and Genetic Structure in Field Populations of Fusarium Species Causing Head Blight in Wheat , 1997 .

[13]  R. Vogel,et al.  Use of the AFLP technique for the Identification and Discrimination of Fusarium graminearum , 1997 .

[14]  C. Alabouvette,et al.  Evaluation of restriction analysis of polymerase chain reaction (PCR)-amplified ribosomal DNA for the identification of Fusarium species , 1997 .

[15]  D. W. Stewart,et al.  Distribution and prediction of Fusarium species associated with maize ear rot in Ontario. , 1997 .

[16]  G. Bateman,et al.  Relationships among Fusarium spp estimated by comparing restriction fragment length polymorphisms in polymerase chain reaction-amplified nuclear rDNA , 1996 .

[17]  T. Gordon,et al.  Relationships among pathogenic and nonpathogenic isolates of Fusarium oxysporum based on the partial sequence of the intergenic spacer region of the ribosomal DNA. , 1996, Molecular plant-microbe interactions : MPMI.

[18]  P. E. Rasmussen,et al.  Diseases of wheat in long-term agronomic experiments at Pendleton, Oregon. , 1996 .

[19]  A. Schilling.  Polymerase chain reaction-based assays for species-specific detection of Fusarium culmorum, F. graminearum, and F. avenaceum , 1996 .

[20]  T. Gordon,et al.  Intraspecific variation within populations of Fusarium oxysporum based on RFLP analysis of the intergenic spacer region of the rDNA. , 1995, Experimental mycology.

[21]  D. Parry,et al.  Fusarium ear blight (scab) in small grain cereals—a review , 1995 .

[22]  D. Parry,et al.  Isolation of Fusarium species from common broad-leaved weeds and their pathogenicity to winter wheat , 1994 .

[23]  K. Seifert,et al.  Genetic characterization of Fusarium graminearum strains using RAPD and PCR amplification , 1993 .

[24]  James B. Anderson,et al.  Molecular phylogeny of northern hemisphere species of Armillaria , 1992 .

[25]  M. Fernandez.  Recovery of Cochliobolus sativus and Fusarium graminearum from living and dead wheat and nongramineous winter crops in southern Brazil , 1991 .

[26]  J. Mattick,et al.  'Touchdown' PCR to circumvent spurious priming during gene amplification. , 1991, Nucleic acids research.

[27]  E. Myers,et al.  Basic local alignment search tool. , 1990, Journal of molecular biology.

[28]  R. Hall,et al.  Transmission of Fusarium graminearum from seed to stems of winter wheat , 1987 .

[29]  A. V. Sturz,et al.  Characterization of Fusarium colonization of spring barley and wheat produced on stubble or fallow soil , 1985 .

[30]  P. E. Nelson,et al.  Fusarium species: an illustrated manual for identification. , 1983 .

[31]  D. Cullen.  Cultural Characteristics, Pathogenicity, and Zearalenone Production by Strains ofGibberella zeaeIsolated from Corn , 1982 .

[32]  L. Burgess,et al.  Distribution of Fusarium roseum ‘Graminearum’ Group 1 and its mode of survival in eastern Australian wheat belt soils , 1977 .

[33]  L. Burgess,et al.  Characteristics of two populations of Fusarium roseum ‘Graminearum’ in Eastern Australia , 1977 .

[34]  R. Francis,et al.  Surveys of Fusaria and Other Fungi Associated with Stalk Rot of Maize in Eastern Australia , 1975 .

[35]  H. Warren.  Fertilization and Wheat Refuse Effects on Fusarium Species Associated with Wheat Roots in Minnesota , 1973 .

[36]  G. Purss.  Pathogenic specialization in Fusarium graminearum , 1971 .