Plasmin effect on platelet glycoprotein Ib-von Willebrand factor interactions.

We have studied the effect of streptokinase on platelets in platelet-rich plasma (PRP) and of plasmin on washed platelets. By three and one-half minutes after the addition of 50,000 IU/mL streptokinase to PRP, the maximum rate of ristocetin-induced platelet agglutination declined 40%, and by 60 minutes, it declined 70%. During the same time interval, the thrombin time increased from 20 seconds to over 120 seconds. At a concentration as low as 50 IU/mL, streptokinase reduced the maximum rate of ristocetin-induced platelet agglutination by 50% and prolonged the thrombin time to 1.5 times control value. Streptokinase added to PRP also caused inhibition of platelet aggregation following stimulation by 2.9 mumol/L adenosine diphosphate, 0.25 U/mL thrombin, and 0.025 mg/mL collagen. Plasmin, 0.05 to 1.0 CU/mL, reduced ristocetin-mediated agglutination of washed platelets in the presence of von Willebrand factor (vWF) from 66% of control to 2% of control, following a one-hour incubation. Autoradiograms produced following sodium dodecyl-polyacrylamide gel electrophoresis (SDS-PAGE) of plasmin-treated 125I-surface-labeled platelets demonstrated progressive loss of a protein with a molecular weight (mol wt) of 180,000; simultaneously, a protein with mol wt 135,000 appeared on autoradiograms produced following SDS-PAGE of the surrounding platelet medium. These proteins are similar in molecular weight to glycoprotein (gp) Ib, a platelet surface receptor for vWF, and glycocalicin, a proteolytic fragment of gpIb. By use of an enzyme-linked immunosorbent assay (ELISA) based immunoinhibition assay for glycocalicin, we were able to demonstrate that plasmin treatment of washed platelets released a glycocalicin-related antigen into the surrounding medium and that appearance of this material corresponding to loss of vWF-dependent, ristocetin-induced agglutination.

[1]  J. Loscalzo,et al.  Partial characterization of a binding site for von Willebrand factor on glycocalicin. , 1986, Blood.

[2]  J. Buring,et al.  Pooled analyses of randomized trials of streptokinase and heparin in phlebographically documented acute deep venous thrombosis. , 1984, The American journal of medicine.

[3]  K. Clemetson,et al.  Antibodies against platelet membrane glycoproteins , 1983, British journal of haematology.

[4]  B. Coller,et al.  Studies with a murine monoclonal antibody that abolishes ristocetin-induced binding of von Willebrand factor to platelets: additional evidence in support of GPIb as a platelet receptor for von Willebrand factor. , 1983, Blood.

[5]  W. Bennett,et al.  Hydrolysis of human platelet membrane glycoproteins with a Serratia marcescens metalloprotease: effect on response to thrombin and von Willebrand factor. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[6]  G. Claeson,et al.  12. A new specific substrate for the determination of plasmin activity , 1982 .

[7]  J. Moake,et al.  Binding of radioiodinated human von Willebrand factor to Bernard-Soulier, thrombasthenic and von Willebrand's disease platelets. , 1980, Thrombosis research.

[8]  D. Collen.  On the Regulation and Control of Fibrinolysis , 1980, Thrombosis and Haemostasis.

[9]  N. O. Solum,et al.  Further evidence for glycocalicin being derived from a larger amphiphilic platelet membrane glycoprotein. , 1980, Thrombosis research.

[10]  N. O. Solum,et al.  Platelet glycocalicin. Its membrane association and solubilization in aqueous media. , 1980, Biochimica et biophysica acta.

[11]  Phillips Dr.  An evaluation of membrane glycoproteins in platelet adhesion and aggregation. , 1980 .

[12]  M. Peterka,et al.  Absence of the 145,000 molecular weight, soluble platelet membrane glycoprotein--lack of platelet agglutination. , 1980, Thrombosis and haemostasis.

[13]  D. Phillips.  An evaluation of membrane glycoproteins in platelet adhesion and aggregation. , 1980, Progress in hemostasis and thrombosis.

[14]  Caen Jp,et al.  The different glycoprotein abnormalities in thrombasthenic and Bernard-Soulier platelets. , 1979 .

[15]  M. Packham,et al.  Survival of rabbit platelets treated in vitro with chymotrypsin, plasmin, trypsin, or neuraminidase. , 1979, Blood.

[16]  G. Jamieson,et al.  Platelet membrane glycoproteins in thrombasthenia, Bernard-Soulier syndrome, and storage pool disease. , 1979, The Journal of laboratory and clinical medicine.

[17]  S. Pizzo,et al.  Demonstration and characterization of specific binding sites for factor VIII/von Willebrand factor on human platelets. , 1979, The Journal of clinical investigation.

[18]  M. Peterka,et al.  Absence of the 145,000 Molecular Weight, Soluble Platelet Membrane Glycoprotein - Lack of Platelet Agglutination , 1979, Thrombosis and Haemostasis.

[19]  A. Nurden,et al.  The different glycoprotein abnormalities in thrombasthenic and Bernard-Soulier platelets. , 1979, Seminars in hematology.

[20]  P. Henriksson,et al.  Effects of leukocytes, plasmin and thrombin on clotting factors. A comparative in vitro study. , 1979, Thrombosis research.

[21]  A. Nurden,et al.  A glycoprotein I defect in the platelets of three patients with severe cirrhosis of the liver. , 1978, Thrombosis research.

[22]  G. Jamieson,et al.  Reduced thrombin binding and aggregation in Bernard-Soulier platelets. , 1978, The Journal of clinical investigation.

[23]  M. Verstraete,et al.  Biochemical Changes Noted during Intermittent Administration of Streptokinase , 1978, Thrombosis and Haemostasis.

[24]  V. Marder,et al.  Effects of enzymatic degradation on the subunit composition and biologic properties of human factor VIII. , 1978, Blood.

[25]  J. Rodés,et al.  Increased factor VIII complex and defective ristocetin-induced platelet aggregation in liver disease. , 1977, Thrombosis research.

[26]  P. Agin,et al.  Platelet plasma membrane glycoproteins. Evidence for the presence of nonequivalent disulfide bonds using nonreduced-reduced two-dimensional gel electrophoresis. , 1977, The Journal of biological chemistry.

[27]  N. O. Solum,et al.  Human platelet glycoproteins. Further evidence that the "GP I band" from whole platelets contains three different polypeptides one of which may be involved in the interaction between platelets and Factor VIII. , 1977, Thrombosis research.

[28]  I. Clemmensen,et al.  The primary inhibitor of plasmin in human plasma. , 1976, The Biochemical journal.

[29]  G. Jamieson,et al.  Platelet glycocalicin. I. Orientation of glycoproteins of the human platelet surface. , 1976, The Journal of biological chemistry.

[30]  C. Lombart,et al.  Platelet glycocalicin. II. Purification and characterization. , 1976, The Journal of biological chemistry.

[31]  N. Aoki,et al.  Isolation and characterization of alpha2-plasmin inhibitor from human plasma. A novel proteinase inhibitor which inhibits activator-induced clot lysis. , 1976, Journal of Biological Chemistry.

[32]  G. Jamieson,et al.  Platelet glycocalicin: a single receptor for platelet aggregation induced by thrombin or ristocetin. , 1976, Thrombosis research.

[33]  M. Verstraete,et al.  Immunochemical distinction between antiplasmin and alpha-antitrypsin. , 1975, Thrombosis research.

[34]  J. Miller,et al.  Plasmin Inhibition of Thrombin-induced Platelet Aggregation , 1975, Thrombosis and Haemostasis.

[35]  J. Allain,et al.  Platelets fixed with paraformaldehyde: a new reagent for assay of von Willebrand factor and platelet aggregating factor. , 1975, The Journal of laboratory and clinical medicine.

[36]  D. Phillips,et al.  Platelet Membrane Glycoproteins Implicated in Ristocetin-Induced Aggregation , 1975, Thrombosis and Haemostasis.

[37]  H. Weiss,et al.  Decreased adhesion of platelets to subendothelium in von Willebrand's disease. , 1974, The Journal of laboratory and clinical medicine.

[38]  J. Caen,et al.  Platelet shape change: induction by trypsin and release of an acidic macroglycopeptide from washed human platelets and from those of three cases of Glanzmann's thrombasthenia. , 1974, Haemostasis.

[39]  A. Nurden.  Platelet macroglycopeptide , 1974, Nature.

[40]  S. Niewiarowski,et al.  Plasmin-induced platelet aggregation and platelet release reaction. Effects on hemostasis. , 1973, The Journal of clinical investigation.

[41]  D. Phillips.  Effect of trypsin on the exposed polypeptides and glycoproteins in the human platelet membrane. , 1972, Biochemistry.

[42]  E. Engvall,et al.  Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. , 1972, Journal of immunology.

[43]  J. Wilson,et al.  Studies on the pathogenesis of the pseudohermaphroditism in the mouse with testicular feminization. , 1972, The Journal of clinical investigation.

[44]  M. Weinstein,et al.  Differential specificities of the thrombin, plasmin and trypsin with regard to synthetic and natural substrates and inhibitors. , 1972, Biochimica et biophysica acta.

[45]  V. Marchesi,et al.  Human erythrocyte membrane glycoprotein: a re-evaluation of the molecular weight as determined by SDS polyacrylamide gel electrophoresis. , 1971, Biochemical and biophysical research communications.

[46]  D. Pepper,et al.  Isolation of a macroglycopeptide from human platelets. , 1970, Biochemistry.

[47]  M. Kopeć,et al.  Inhibition of Platelet Aggregation by Dialysable Fibrinogen Degradation Products (FDP) , 1970, Thrombosis and Haemostasis.

[48]  U. K. Laemmli,et al.  Cleavage of structural proteins during , 1970 .

[49]  D Rodbard,et al.  Rapid calculation of radioimmunoassay results. , 1969, The Journal of laboratory and clinical medicine.

[50]  Y. Sultan,et al.  Congenital bleeding disorders with long bleeding time and normal platelet count. II. Von Willebrand's disease (report of thirty-seven patients). , 1968, The American journal of medicine.

[51]  M. B. Zucker,et al.  Some Effects of Fibrinogen Degradation Products (FDP) on Blood Platelets , 1966, Thrombosis and Haemostasis.

[52]  A. Fletcher,et al.  ABNORMAL PLASMINOGEN-PLASMIN SYSTEM ACTIVITY (FIBRINOLYSIS) IN PATIENTS WITH HEPATIC CIRRHOSIS: ITS CAUSE AND CONSEQUENCES. , 1964, The Journal of clinical investigation.

[53]  G. Born,et al.  Effects of inorganic ions and of plasma proteins on the aggregation of blood platelets by adenosine diphosphate , 1964, The Journal of physiology.

[54]  H. D. Anderson,et al.  The Preparation of Human Fibrinolysin (Plasmin) * , 1960, Vox sanguinis.

[55]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[56]  H. Naim,et al.  Relationship between glycocalicin and glycoprotein lb of human platelets ( Factor VIII-related von Willebrand factor receptor / platelet glycoproteins / peanut agglutinin affinity chromatography / radioactive peptide map ) , 2022 .