Innovative and Sustainable Production of Biopolymers

This work aims to develop methodologies and tools to support the design and management of sustainable processes for the production of biodegradable polyhydroxyalcanoates (PHAs) biopolymers. PHAs are linear polyesters produced in nature by bacteria through aerobic fermentation of many carbon sources, completely biodegradable and biocompatible. We carried out a study inherent to the advancement of an innovative, cost-effective and environmentally sustainable technology for isolating PHAs from bacteria mixed cultures by combining: (a) innovative cells’ pre-treatments and polymer purification’s strategy by means of TiO2/UV or Ag0 nanostructured materials; (b) polymer extraction through a green and safe system directly applicable to bacterial cultures, which combines the advantages of solvent extraction and these of dissolution of the non-PHAs cellular matrix through surfactants; (c) monitoring and control tools for process energy and efficiency management. The outcomes put the basis for the design and subsequent building of a working pilot system for the production of completely biodegradable and biocompatible PHAs. The efficiency can be improved and the investments and operating costs can be decreased thanks to the optimization of the production process with the introduction of safe and cheap PHAs extraction route without use of toxic and harmful chemicals and the integration of monitoring and automation tools. The engineering and integration of nano-TiO2 phase within textile fibres and their use as photocatalytic active media for bacteria pre- and post-treatment of waste water added a new opportunity for improving process efficiency and sustainability.

[1]  Vishwas G. Pangarkar,et al.  Photocatalytic degradation for environmental applications – a review , 2002 .

[2]  Gerard Muyzer,et al.  Enrichment of a mixed bacterial culture with a high polyhydroxyalkanoate storage capacity. , 2009, Biomacromolecules.

[3]  S. Popova,et al.  On—Line State and Parameters Estimation Based Measurements of the Glucose in Mixed Culture System , 2006 .

[4]  B. Witholt,et al.  Closed-loop control of bacterial high-cell-density fed-batch cultures: production of mcl-PHAs by Pseudomonas putida KT2442 under single-substrate and cofeeding conditions. , 1999, Biotechnology and bioengineering.

[5]  Chi-Wei Lo,et al.  Isolation and purification of bacterial poly(3-hydroxyalkanoates) , 2008 .

[6]  Ralf Cord-Ruwisch,et al.  Online detection of feed demand in high cell density cultures of Escherichia coli by measurement of changes in dissolved oxygen transients in complex media , 2004, Biotechnology and bioengineering.

[7]  J. Sun,et al.  Recent developments in heterogeneous photocatalytic water treatment using visible light-responsive photocatalysts: a review , 2015 .

[8]  C. Torri,et al.  Extraction of polyhydroxyalkanoates from mixed microbial cultures: Impact on polymer quality and recovery. , 2015, Bioresource technology.

[9]  Maria A M Reis,et al.  The influence of process parameters on the characteristics of polyhydroxyalkanoates produced by mixed cultures. , 2008, Macromolecular bioscience.

[10]  Mark C M van Loosdrecht,et al.  Waste to resource: Converting paper mill wastewater to bioplastic. , 2012, Water research.

[11]  A. Barbetta,et al.  Polyhydroxyalkanoates production with mixed microbial cultures: from culture selection to polymer recovery in a high-rate continuous process. , 2014, New biotechnology.

[12]  Julián Blanco,et al.  Decontamination and disinfection of water by solar photocatalysis: Recent overview and trends , 2009 .

[13]  Suchada Chanprateep,et al.  Current trends in biodegradable polyhydroxyalkanoates. , 2010, Journal of bioscience and bioengineering.

[14]  Cristian Torri,et al.  Dimethyl carbonate and switchable anionic surfactants: two effective tools for the extraction of polyhydroxyalkanoates from microbial biomass , 2015 .

[15]  M C M Van Loosdrecht,et al.  Production of polyhydroxyalkanoates by mixed culture: recent trends and biotechnological importance. , 2004, Biotechnology advances.

[16]  R. L. Sawhney,et al.  Treatment of Hazardous Organic and Inorganic Compounds through Aqueous-Phase Photocatalysis: A Review , 2004 .

[17]  C. Saint,et al.  Recent developments in photocatalytic water treatment technology: a review. , 2010, Water research.

[18]  M. Reis,et al.  The impact of pH control on the volumetric productivity of mixed culture PHA production from fermented molasses , 2014 .

[19]  T. Ojeda.  Polymers and the Environment , 1997 .

[20]  A. Werker,et al.  Thermal properties and crystallization behavior of fractionated blocky and random polyhydroxyalkanoate copolymers from mixed microbial cultures , 2014 .

[21]  R. Newman,et al.  Physico-chemical properties of polyhydroxyalkanoate produced by mixed-culture nitrogen-fixing bacteria , 2009, Applied Microbiology and Biotechnology.