Intracutaneous anthrax infection in wild rodents.
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Received for publication May 1, 1957. This work was supported by U. S. Army Chemical Corps contract, No. DA-18-064-CML2639, with the University of Utah. was obtained from Dr. George C. Wright, Fort Detrick, Frederick, Maryland. Upon arrival the suspension was diluted 1:100 in buffered saline (pH 7.0; 0.1M phosphate buffer) and stored at 4 C. From this stock suspension the "challenge" dilutions were prepared in 0.85% sodium chloride. Challenge injections.?Each species was divided into five groups of eight animals each, except where an inadequate number of animals necessitated the use of fewer groups and/or fewer animals per group. The spore suspension was injected intracutaneously into a region on the abdomen from which the fur had been plucked just prior to inoculation. In each instance a tuberculin syringe with a 27 gauge needle was used to administer the inoculum. The animals of
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[2] W. J. Cromartie,et al. Studies on infection with Bacillus anthracis; chemical and immunological properties of the protective antigen in crude extracts of skin lesions of B. anthracis. , 1947, The Journal of infectious diseases.