Clinical assessment of oral malodor using autofluorescence of tongue coating.

OBJECTIVES The aim of this study was to evaluate whether a new method using quantitative light-induced fluorescence-digital (QLF-D) was appropriate for the diagnosis of oral malodor by quantifying the fluorescence of tongue coating. METHODS This study examined 103 healthy subjects who have an oral malodor as a main complaint. The levels of oral malodor were measured by organoleptic scores (OLS) and volatile sulfur compound (VSC) levels. The fluorescent tongue coating images captured by QLF-D were quantified as the integrated fluorescence score (IF score) by multiplying the intensity and area of fluorescence. The correlations between the fluorescence parameters and OLS as well as VSC levels and the diagnostic accuracy of the IF score were evaluated. RESULTS The IF score of tongue coating showed a significant positive correlation with the OLS (r=0.54, p<0.01) and the VSC levels (r=0.49, p<0.01). This score was significantly differed with the level of oral malodor (p<0.001), and its AUC was 0.72 in identifying the patient with definite oral malodor (≥OLS 2). CONCLUSIONS A new method quantifying tongue coating fluorescence detected by QLF-D can be used to diagnose oral malodor and assess its severity in the clinical practice.

[1]  K. Yaegaki,et al.  Biochemical and clinical factors influencing oral malodor in periodontal patients. , 1992, Journal of periodontology.

[2]  J Tonzetich,et al.  Production and origin of oral malodor: a review of mechanisms and methods of analysis. , 1977, Journal of periodontology.

[3]  T Takehara,et al.  Correlation between volatile sulphur compounds and certain oral health measurements in the general population. , 1995, Journal of periodontology.

[4]  K. Yaegaki,et al.  Volatile sulfur compounds in mouth air from clinically healthy subjects and patients with periodontal disease. , 1992, Journal of periodontal research.

[5]  M Rosenberg,et al.  Reproducibility and Sensitivity of Oral Malodor Measurements with a Portable Sulphide Monitor , 1991, Journal of dental research.

[6]  D. Hinode,et al.  Relationship between halitosis and periodontal disease - associated oral bacteria in tongue coatings. , 2014, International journal of dental hygiene.

[7]  K. Yaegaki,et al.  Tongue brushing and mouth rinsing as basic treatment measures for halitosis. , 2002, International Dental Journal.

[8]  M. Quirynen,et al.  Instrumental assessment of halitosis for the general dental practitioner , 2014, Journal of breath research.

[9]  M Quirynen,et al.  Management of oral malodour. , 2003, Journal of clinical periodontology.

[10]  M. Sanz,et al.  Biofilms and the tongue: therapeutical approaches for the control of halitosis , 2003, Clinical Oral Investigations.

[11]  Si-Mook Kang,et al.  Association between the cariogenicity of a dental microcosm biofilm and its red fluorescence detected by Quantitative Light-induced Fluorescence-Digital (QLF-D). , 2013, Journal of dentistry.

[12]  G. V. D. Van der Weijden,et al.  Tongue coating and salivary bacterial counts in healthy/gingivitis subjects and periodontitis patients. , 2001, Journal of clinical periodontology.

[13]  Wim Teughels,et al.  Clinical reliability of non-organoleptic oral malodour measurements. , 2009, Journal of clinical periodontology.

[14]  E. de Josselin de Jong,et al.  Photobleaching of red fluorescence in oral biofilms. , 2011, Journal of periodontal research.

[15]  J. D. de Soet,et al.  Red Autofluorescence of Dental Plaque Bacteria , 2006, Caries Research.

[16]  Misaki Ohmori,et al.  The relationship between physiologic halitosis and periodontopathic bacteria of the tongue and gingival sulcus , 2010, Odontology.

[17]  T. Attin,et al.  The Ability of Selected Oral Microorganisms to Emit Red Fluorescence , 2005, Caries Research.

[18]  J Bagg,et al.  Microbiological culture analysis of the tongue anaerobic microflora in subjects with and without halitosis. , 2005, Oral diseases.

[19]  M Rosenberg,et al.  Relationship of oral malodor to periodontitis: evidence of independence in discrete subpopulations. , 1994, Journal of periodontology.

[20]  Eun-Song Lee,et al.  Monitoring the maturation process of a dental microcosm biofilm using the Quantitative Light-induced Fluorescence-Digital (QLF-D). , 2014, Journal of dentistry.

[21]  T. Ueda,et al.  New method for evaluation of tongue-coating status. , 2007, Journal of oral rehabilitation.

[22]  F C Groppo,et al.  The relationship between volatile sulphur compounds, tongue coating and periodontal disease. , 2009, International journal of dental hygiene.

[23]  E. D. De Boever,et al.  Assessing the contribution of anaerobic microflora of the tongue to oral malodor. , 1995, Journal of the American Dental Association.

[24]  F. Dewhirst,et al.  Diversity of Bacterial Populations on the Tongue Dorsa of Patients with Halitosis and Healthy Patients , 2003, Journal of Clinical Microbiology.

[25]  M. B. Edlund,et al.  The formation of hydrogen sulfide and methyl mercaptan by oral bacteria. , 1990, Oral microbiology and immunology.

[26]  J Egelberg,et al.  Evaluation of tongue coating indices. , 2007, Oral diseases.

[27]  Philip Smith,et al.  The Microbiological Origin of Fluorescence Observed in Plaque on Dentures during QLF Analysis , 2006, Caries Research.

[28]  H L Wang,et al.  Association between oral malodor and adult periodontitis: a review. , 2001, Journal of clinical periodontology.

[29]  Hakan Çolak,et al.  Halitosis: From diagnosis to management , 2013, Journal of natural science, biology, and medicine.

[30]  L. Feenstra,et al.  A review of the current literature on aetiology and measurement methods of halitosis. , 2007, Journal of dentistry.

[31]  I A Pretty,et al.  The intra- and inter-examiner reliability of quantitative light-induced fluorescence (QLF) analyses , 2002, British Dental Journal.

[32]  G. V. D. Van der Weijden,et al.  Tongue coating and tongue brushing: a literature review. , 2003, International journal of dental hygiene.

[33]  Jumpei Washio,et al.  Hydrogen sulfide-producing bacteria in tongue biofilm and their relationship with oral malodour. , 2005, Journal of medical microbiology.

[34]  C. Volgenant,et al.  Effect of metalloporphyrins on red autofluorescence from oral bacteria. , 2013, European journal of oral sciences.