Ecological security is the main task and applied field of present geography, resources and environment sciences and ecology.Ecological security evaluation will efficiently promote ecological security and environmental construction in regional land use. In this thesis, the authors put forward the index system of ecological security evaluation in karst mountainous area on three aspects, the pressure of resources and eco-environment, the quality of resources and eco-environment, and the ability of environmental protection and ecological improvement. Using the evaluation method with single index, based on the case study of Du’an Yao Autonomous County of Guangxi Zhuang Autonomous Region, the system of synthetic regionalization of ecological agricultural economy was formed, which includes three regions, south region with basic security and synthetic agricultural development, east region with critical security and agriculture, forestry, animal husbandry balanced development, mid-west and south region with ecological insecurity and compounded agriculture and forestry management. Meanwhile, for these regions, the countermeasures of sustainable agricultural development were pointed out, which provide the basis and example for ecological regulation and control of sustainable agricultural development in counties of karst mountainous area.