Proof-of-principle demonstration of a Mueller matrix decomposition method for polarized light tissue characterization in vivo.

We demonstrate the first in vivo use of a Mueller matrix decomposition method for polarization-based characterization of tissue. Collagenase is injected into a region of dermal tissue in a dorsal skin window chamber in a nude mouse to alter the structure of the extracellular matrix. Mueller matrices for polarized light transmitted through the window chamber in the collagenase-treated region, as well as a distal control region, are measured. From the measured matrices, the individual constituent polarization properties of the tissue are extracted through polar matrix decomposition. Large decreases in birefringence and depolarization are seen in the collagenase-treated region due to the destruction of collagen, showing the potential for this method to monitor the organization and structural anisotropy of tissue. This study represents the first in vivo demonstration of a Mueller matrix decomposition method for polarimetric tissue characterization.

[1]  M J Everett,et al.  Mapping of Birefringence and Thermal Damage in Tissue by use of Polarization-Sensitive Optical Coherence Tomography. , 1998, Applied optics.

[2]  J. Seltzer,et al.  Cleavage of type VII collagen by interstitial collagenase and type IV collagenase (gelatinase) derived from human skin. , 1989, The Journal of biological chemistry.

[3]  Xinxin Guo,et al.  Angular measurements of light scattered by turbid chiral media using linear Stokes polarimeter. , 2006, Journal of biomedical optics.

[4]  V. Duance,et al.  Type I and III collagen content and fibre distribution in normal human skin during ageing , 1987, The British journal of dermatology.

[5]  Nirmalya Ghosh,et al.  Polarized Light Assessment of Complex Turbid Media Such as Biological Tissues Using Mueller Matrix Decomposition , 2010 .

[6]  R. Chipman,et al.  Interpretation of Mueller matrices based on polar decomposition , 1996 .

[7]  Adrian Mariampillai,et al.  Intravital high-resolution optical imaging of individual vessel response to photodynamic treatment. , 2008, Journal of biomedical optics.

[8]  Nirmalya Ghosh,et al.  Mueller matrix decomposition for extraction of individual polarization parameters from complex turbid media exhibiting multiple scattering, optical activity, and linear birefringence. , 2008, Journal of biomedical optics.

[9]  Yoichi Hirashima,et al.  Recursive Filter Design for Estimating Time Varying Multijoint Human Arm Viscoelasticity , 2006, Int. J. Comput. Syst. Signals.

[10]  Xinxin Guo,et al.  Polarized light propagation in multiply scattering media exhibiting both linear birefringence and optical activity: Monte Carlo model and experimental methodology. , 2007, Journal of biomedical optics.

[11]  Lihong V. Wang,et al.  Propagation of polarized light in birefringent turbid media: a Monte Carlo study. , 2002, Journal of biomedical optics.

[12]  Lihong V. Wang,et al.  Jones-matrix imaging of biological tissues with quadruple-channel optical coherence tomography. , 2002, Journal of biomedical optics.

[13]  Steven L. Jacques,et al.  Special Section Guest Editorial: Tissue Polarimetry , 1999 .