The Gene Cloning and Bioactivity of the Expression Product of the Human FKBP12

To obtain bioactive hFKBP12 protein for screening novel neurotrophic drugs,RT PCR,computer aided gene expression discriminating model and M.W.cut off ultrafiltration were used.hFKBP12 gene was cloned successfully and then redesigned optimally according to the high expression model for the foreign gene.The redesigned gene was highly expressed in pBV220 vector as inclusion bodies,which were renatured and purified to only one band on SDS PAGE.The purified hFKBP12 showed peptidyl prolyl cis trans isomerase (PPIase) activity.It indicated that recombinant hFKBP12 was bioactive just as its wild type's.