The negatively charged protein extracted from Tetrahymena pyriformis as an attractant in Amoeba proteus chemotaxis

We extracted a pure protein from Tetrahymena pyriformis as the chemotactic substance in Amoeba proteus. The protein was heat‐stable, negatively charged at neutral pH (since its isoelectric point was near pH 4.5) and composed of three subunits. Its molecular weight was 3 × 8,000.