Preparation of monospecific HLA antibodies from cross-reactive multispecific antisera.

Multispecific, cross-reactive sera can be demonstrated by absorption studies to be of two types; a single antibody that is multivalent, and some that are mixtures of monospecific and multispecific antibody populations. The second type can be separated by a differential absorption technique. The characteristics of these sera that can be separated and the techniques are discussed.