DETECTION OF FUNGAL DNA IN THE MIDDLE EAR EFFUSION OF PATIENTS SUFFERING FROM OTITIS MEDIA WITH EFFUSION

Background: Otitis media with effusion (OME) is common in the children. It is proven that pathogenic bacteria as a causative agent of middle ear effusion, however the role of fungal infection in otitis media with effusion remains unclear. Therefore, this study was conducted to assess presence of fungi in OME. Methods: From January 2005 to September 2006, a number of 62 children with proven otitis media with effusion subjected to the case series study at Amiralmomenin Hospital in Rasht City, Iran. After myringotomy, middle ear effusion was collected. In 48 patients, both ears demonstrated effusion, whereas in 14 patients, only one ear had effusion. Standard mycological culture and polymerase chain reaction (PCR) assay were performed in 110 and 79 samples, respectively. Results: The growth of fungi was observed in 9 samples (8.8%). The result of our PCR assay showed that 23 samples (29.1%) were positive for fungal DNA. Conclusion: Middle ear effusion from cases with OME contains fungi and it might play a role in the pathogens of OME. PCR assay is a better indicator in detection of fungus in middle ear effusion, compared with fungal culturing method. However, the estimation of its sensitivity and specificity in detection of fungal agents in these patients needs more molecular epidemiological studies.

[1]  S. Pelton,et al.  Recent Advances in Otitis Media , 2009, The Pediatric infectious disease journal.

[2]  R. Bento,et al.  Craniofacial morphology and otitis media with effusion in children. , 2008, International journal of pediatric otorhinolaryngology.

[3]  S. Vijayasekaran,et al.  The role of chronic infection in children with otitis media with effusion: Evidence for intracellular persistence of bacteria , 2008, Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery.

[4]  N. Papadopoulos,et al.  Human rhinoviruses in otitis media with effusion , 2006, Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology.

[5]  J. Sok,et al.  Basic Otorhinolaryngology: A Step-by-Step Learning Guide, 2nd ed. , 2006 .

[6]  Maria José Figueras,et al.  Atlas of clinical fungi. , 2005 .

[7]  J. J. Grote,et al.  Otitis media with effusion: disease or defense? A review of the literature. , 2004, International journal of pediatric otorhinolaryngology.

[8]  R. Rosenfeld,et al.  Otitis media and speech and language: a meta-analysis of prospective studies. , 2004, Pediatrics.

[9]  A. Lalwani,et al.  Screening of middle ear effusion for the common sinus pathogen Bipolaris , 2003, European Archives of Oto-Rhino-Laryngology.

[10]  A. Lalwani,et al.  Detection of Fungal DNA in Effusion Associated With Acute and Serous Otitis Media , 2002, The Laryngoscope.

[11]  J. Post,et al.  Candidate's Thesis: Direct Evidence of Bacterial Biofilms in Otitis Media , 2001, The Laryngoscope.

[12]  M. Doymaz,et al.  Bacteriological and PCR analysis of clinical material aspirated from otitis media with effusions. , 2001, International journal of pediatric otorhinolaryngology.

[13]  A. Lalwani,et al.  Detection of Fungi in the Nasal Mucosa Using Polymerase Chain Reaction , 2001, The Laryngoscope.

[14]  E. Moyse,et al.  Viral RNA in middle ear mucosa and exudates in patients with chronic otitis media with effusion. , 2000, Archives of otolaryngology--head & neck surgery.

[15]  M. Mildner,et al.  Characterization of a cDNA clone, encoding a 70 kDa heat shock protein from the dermatophyte pathogen Trichophyton rubrum. , 2000, Gene.

[16]  S. Tsai,et al.  Effects of indoor environmental factors on risk for acute otitis media in a subtropical area. , 1999, Journal of toxicology and environmental health. Part A.

[17]  P. Van cauwenberge,et al.  Recent advances in otitis media. Definition and classification. , 1989, The Annals of otology, rhinology & laryngology. Supplement.

[18]  J. Klein,et al.  Otitis media in infants and children , 1988 .

[19]  C. Sweet.  OTITIS MEDIA IN INFANTS AND CHILDREN. , 1923, California state journal of medicine.