Quantitative Analysis of 1-deoxynojirimycin in Mulberry Leaves

Countries of the world are competing for the collection and utilization of genetic resources, which is a resource of valueadded industrialization. We are building database of silkworm and mulberry resources with morphological, genetic characteristics data etc. Moreover, recent bioactive compound has been receiving increasing attention. So we analyzed 1deoxynojirimycin(1-DNJ) content as a this item in addition to the basic information using 363 strains(varieties) of mulberry genetic resources. They were grown under the same environment and conditions. Mulberry leaves were collected and then freeze-dried and powdered for 1-DNJ test. As a result, 1-DNJ mean content of 363 mulberry strains was 0.176±0.077%, and the coefficient of variation (CV) was 43.5%. The variation between the strains was greatly severe. Among the tested strains, ‘C1D89/29’ was showed the highest content of 0.47%, whereas ‘Pumbo 24’ and ‘Turkey E’ were showed the lowest content of 0.05% respectively. The content of 1-DNJ of 16 mulberry varieties for silkworm rearing were compared. Average concentration was 0.17±0.04%, and the coefficient of variation (CV) was 22.8%. Variation among cultivars was not significant. Finally we selected 1-DNJ high-containing 11 strains. They are as follows. ‘C1D89/29’, ‘Hiihak’, ‘Jeokasibmunja’, ‘Gweonchil’, ‘Botongsibmunja’, ‘Jeokchuk’, ‘bulguksang’, ‘Geunsookgojo', ‘Busanggeum’, ‘Guksang 20’ and ‘Taekwang’ which are more than twice of the overall average content.