Correlation Between Lead Exposure Indicators and Sister Chromatid Exchange (SCE) Frequencies in Lymphocytes from Inorganic Lead Exposed Workers

Inorganic lead exposure was studied in 31 volunteers employed in storage battery plant. The genotoxicity of lead was measured in terms of sister chromatid exchange (SCE). Erythrocyte δ-aminolevulinic acid dehydrogenase (ALAD) activity, urinary δ-aminolevulinic acid (U-ALA), and blood lead levels (PbBs) were also determined to evaluate some possible relations between these lead exposure indicators and the observed SCE frequencies. Blood lead concentration of 36.31 μg/dl was determined as an average level in the workers. Consequently decreased ALAD activity in erythrocytes and increased U-ALA excretion was observed in statistically higher PbBs when compared with the control group. A statistically significant correlation was observed between the PbBs and SCE frequencies (p < 0.05). Moreover, the correlation between U-ALA excretion and SCE frequencies (p < 0.01) was relatively higher than the correlation between PbBs and SCE frequencies. These results might indicate a possible mechanism of ALA mediation in the genotoxic effects of lead.

[1]  Y. Hiraku,et al.  Mechanism of Oxidative DNA Damage Induced by δ-Aminolevulinic Acid in the Presence of Copper Ion , 1996 .

[2]  M. Markowitz.  Lead poisoning. , 2000, Pediatrics in review.

[3]  D. Rempel.  The lead-exposed worker. , 1989, JAMA.

[4]  M. Hermes-Lima,et al.  Are free radicals involved in lead poisoning? , 1991, Xenobiotica; the fate of foreign compounds in biological systems.

[5]  R. Marcos,et al.  Biomonitoring of workers exposed to lead. Genotoxic effects, its modulation by polyvitamin treatment and evaluation of the induced radioresistance. , 1998, Mutation research.

[6]  A. Aydın,et al.  Urinary Excretion of Total and Inorganic Lead in Tetraethyllead Exposed Workers , 1998, Bulletin of environmental contamination and toxicology.

[7]  Randall C.Baselt,et al.  Biological monitoring methods for industrial chemicals , 1980 .

[8]  K. Schaller,et al.  European standardized method for the determination of delta-aminolevulinic acid dehydratase activity in blood. , 1974, Zeitschrift fur klinische Chemie und klinische Biochemie.

[9]  T. Sakai,et al.  δ-Aminolevulinic acid in plasma or whole blood as a sensitive indicator of lead effects, and its relation to the other home-related parameters , 1996 .

[10]  C. Pellicciari,et al.  Lead-induced changes in the stabilization of the mouse sperm chromatin. , 1988, Toxicology.

[11]  C Winder,et al.  The genotoxicity of lead. , 1993, Mutation research.

[12]  A. Trevisan.  Concentration adjustment of spot samples in analysis of urinary xenobiotic metabolites. , 1990, American journal of industrial medicine.

[13]  M. Ogata,et al.  Simple method for determination of urinary -aminolevulinic acid as an index of lead exposure. , 1972, Clinical chemistry.

[14]  H. Wichmann,et al.  The effect of low-level blood lead on hematologic parameters in children. , 2000, Environmental research.

[15]  W. Sanger,et al.  Increased frequency of sister-chromatid exchanges in alcoholics. , 1981, Mutation research.

[16]  Wulf Hc.  Monitoring of genotoxic exposure of humans by the sister chromatid exchange test. Methodology and confounding factors. , 1990 .

[17]  D. Houston,et al.  Does vitamin C intake protect against lead toxicity? , 2009, Nutrition reviews.

[18]  J. H. Li,et al.  Genetic toxicology of lead compounds. , 1988, Carcinogenesis.

[19]  G. Gerber,et al.  Chromosomal aberrations in bone-marrow cells of mice given a normal or a calcium-deficient diet supplemented with various heavy metals. , 1979, Mutation research.

[20]  E. Bechara,et al.  Correlation between plasma 5-aminolevulinic acid concentrations and indicators of oxidative stress in lead-exposed workers. , 1997, Clinical chemistry.

[21]  A. Hartwig,et al.  Indirect mechanism of lead-induced genotoxicity in cultured mammalian cells. , 1990, Mutation research.

[22]  S. Wolff,et al.  New Giemsa method for the differential staining of sister chromatids , 1974, Nature.