Short-Term Effects of Two COX-2 Selective Non-Steroidal Anti-Inflammatory Drugs on the Release of Growth Factors and Cytokines from Canine Platelet-Rich Gel Supernatants

(1) Background: There is a lack of knowledge about how a single dose of COX-2 selective non-steroidal anti-inflammatory drugs (NSAIDs) might affect the release of growth factors (GFs) and cytokines from canine platelet-rich gels (PRGs) and other hemocomponents. (2) Methods: A crossover study was conducted in six adult mongrel dogs. Animals were randomized to receive a single dose of either carprofen or firocoxib. PRG, temperature-induced platelet lysate (TIPL), chemically induced PL (CIPL), and plasma hemocomponents were obtained from each dog before (1 h) and after (6 h) the treatments. Platelet and leukocyte counts and determination of the concentrations of platelet-derived growth factor-BB, (PDGF-BB), transforming growth factor beta-1 (TGF-β1), interleukin 1 beta (IL-1β), tumor necrosis factor-alpha (TNF-α) and IL-10 concentrations were assayed by ELISA in all hemocomponents. (3) Results: Both platelet and leukocyte counts and PDGF-BB concentrations were not affected by NSAIDs and time. Total TGF-β1 concentrations were not affected by NSAIDs; however, the release of this GF was increased in PRG supernatants (PRGS) at 6 h. IL-1β and TNF-α concentrations were significantly (p < 0.001) lower in both firocoxib PRGS and plasma at 6 h, respectively. IL-10 concentrations were significantly (p < 0.001) lower at 6 h in all hemocomponents treated with both NSAIDs. (4) Conclusions: The clinical implications of our findings could indicate that these drugs should be withdrawn from patients to allow their clearance before the clinical use of PRP/PRG. On the other hand, the prophylactic use of NSAIDs to avoid the inflammatory reactions that some patients might have after PRP/PRG treatment should be performed only in those animals with severe reactive inflammation to the treatment.

[1]  H. Abrahamse,et al.  Targeting Wnt/β-catenin signaling and its interplay with TGF-β and Notch signaling pathways for the treatment of chronic wounds , 2024, Cell communication and signaling : CCS.

[2]  Xiuyue Qiu,et al.  Exploring causal correlations between inflammatory cytokines and knee osteoarthritis: a two-sample Mendelian randomization , 2024, Frontiers in immunology.

[3]  R. Rakoczy,et al.  The Effect of a Rotating Magnetic Field on the Regenerative Potential of Platelets , 2024, International journal of molecular sciences.

[4]  A. Colbath,et al.  Literature review details and supports the application of platelet-rich plasma products in canine medicine, particularly as an orthobiologic agent for osteoarthritis. , 2024, Journal of the American Veterinary Medical Association.

[5]  Jaclyn R Missanelli,et al.  Single-dose nonsteroidal anti-inflammatory drugs in horses have no impact on concentrations of cytokines or growth factors in autologous protein solution and platelet-rich plasma. , 2024, American journal of veterinary research.

[6]  Lei Yao,et al.  Platelet-Rich Plasma Has Better Results for Long-term Functional Improvement and Pain Relief for Lateral Epicondylitis: A Systematic Review and Meta-analysis of Randomized Controlled Trials. , 2024, The American journal of sports medicine.

[7]  Xiaogang Huang,et al.  Current advancements in therapeutic approaches in orthopedic surgery: a review of recent trends , 2024, Frontiers in bioengineering and biotechnology.

[8]  Oscar Covarrubias,et al.  Platelet rich plasma therapy versus other modalities for treatment of plantar fasciitis: A systematic review and meta-analysis. , 2024, Foot and ankle surgery : official journal of the European Society of Foot and Ankle Surgeons.

[9]  Liangbo Zhu,et al.  Platelet-rich plasma in orthopedics: Bridging innovation and clinical applications for bone repair , 2024, Journal of orthopaedic surgery.

[10]  Hai‐wei Xu,et al.  Increased Expression of Inflammatory Cytokines and Discogenic Neck Pain , 2023, Orthopaedic surgery.

[11]  V. Villar-Suárez,et al.  Regenerative Medicine Applied to Musculoskeletal Diseases in Equines: A Systematic Review , 2023, Veterinary sciences.

[12]  Takayuki Okumo,et al.  Multifactorial Comparative Analysis of Platelet-Rich Plasma and Serum Prepared Using a Commercially Available Centrifugation Kit , 2023, Cureus.

[13]  Rafee Alnajjar,et al.  Current Status and Advancements in Platelet-Rich Plasma Therapy , 2023, Cureus.

[14]  C. Suarez,et al.  Biotherapeutic Applications of Platelet-Rich Plasma in Regenerative Medicine , 2023, Tissue Engineering and Regenerative Medicine.

[15]  M. Carluccio,et al.  Progress in Regenerative Medicine: Exploring Autologous Platelet Concentrates and Their Clinical Applications , 2023, Genes.

[16]  S. Kapetanakis,et al.  Platelet-Rich Plasma for Degenerative Spine Disease: A Brief Overview , 2023, Spine surgery and related research.

[17]  H. Rahmatullah Bin Abd Razak,et al.  Three doses of PRP therapy may be more effective than one dose of Platelet-Rich Plasma (PRP) in the Treatment of Knee Osteoarthritis: A Systematic Review and Meta-analysis. , 2023, Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association.

[18]  A. Kaneps A one-health perspective: use of hemoderivative regenerative therapies in canine and equine patients. , 2023, Journal of the American Veterinary Medical Association.

[19]  Yi Sun,et al.  Combination of PDGF-BB and adipose-derived stem cells accelerated wound healing through modulating PTEN/AKT pathway. , 2023, Injury.

[20]  R. Smith,et al.  Tumour necrosis factor alpha, interleukin 1 beta and interferon gamma have detrimental effects on equine tenocytes that cannot be rescued by IL-1RA or mesenchymal stromal cell–derived factors , 2022, Cell and Tissue Research.

[21]  P. Vongchan,et al.  In Vitro Assessment of Lyophilized Advanced Platelet-Rich Fibrin from Dogs in Promotion of Growth Factor Release and Wound Healing , 2022, Veterinary sciences.

[22]  A. Ceballos-Márquez,et al.  Temporal Release and Denature of Several Mediators in Pure Platelet-Rich Plasma and Temperature-Induced Platelet Lysates Derived from a Similar Bovine Platelet Concentrate , 2022, Veterinary medicine international.

[23]  J. Gines Effect of Leukoreduced Platelet Rich Plasma on Intra-Articular Pro-Inflammatory Cytokines in a Canine Pilot Study , 2022, Animals : an open access journal from MDPI.

[24]  J. U. Carmona,et al.  Evaluation of the Pro-, Anti-Inflammatory, and Anabolic Effects of Autologous Platelet-Rich Gel Supernatants in an in vitro Coculture System of Canine Osteoarthritis , 2022, Veterinary medicine international.

[25]  R. Berghaus,et al.  Evidence for anti‐inflammatory effects of firocoxib administered to mares with experimentally induced placentitis , 2021, American journal of reproductive immunology.

[26]  R. Todhunter,et al.  Spontaneous dog osteoarthritis — a One Medicine vision , 2019, Nature Reviews Rheumatology.

[27]  Brendan H. Lee,et al.  Effects of Aspirin on Growth Factor Release From Freshly Isolated Leukocyte-Rich Platelet-Rich Plasma in Healthy Men: A Prospective Fixed-Sequence Controlled Laboratory Study , 2019, The American journal of sports medicine.

[28]  Stephanie Kelly,et al.  Penetration and efficacy of transdermal NSAIDs in a model of acute joint inflammation , 2018, Journal of Pain Research.

[29]  Anna Rita Attili,et al.  Platelet-rich Plasma and Other Hemocomponents in Veterinary Regenerative Medicine. , 2018, Wounds : a compendium of clinical research and practice.

[30]  J. U. Carmona,et al.  Influence of calcium salts and bovine thrombin on growth factor release from equine platelet-rich gel supernatants , 2016, Veterinary and Comparative Orthopaedics and Traumatology.

[31]  C. Martínez,et al.  The influence of platelet-derived products on angiogenesis and tissue repair: a concise update , 2015, Front. Physiol..

[32]  A. Teixeira-Carvalho,et al.  Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining , 2015, Acta Veterinaria Scandinavica.

[33]  A. McCoy Animal Models of Osteoarthritis , 2015, Veterinary pathology.

[34]  S. Kohl,et al.  Platelet-rich Concentrates Differentially Release Growth Factors and Induce Cell Migration In Vitro , 2015, Clinical orthopaedics and related research.

[35]  M. Mitchell,et al.  Grazing dairy cows had decreased interferon-γ, tumor necrosis factor, and interleukin-17, and increased expression of interleukin-10 during the first week after calving. , 2015, Journal of dairy science.

[36]  D. M. Dohan Ehrenfest,et al.  Classification of platelet concentrates (Platelet-Rich Plasma-PRP, Platelet-Rich Fibrin-PRF) for topical and infiltrative use in orthopedic and sports medicine: current consensus, clinical implications and perspectives. , 2014, Muscles, ligaments and tendons journal.

[37]  Dariusz Szukiewicz,et al.  The Role of Inflammatory and Anti-Inflammatory Cytokines in the Pathogenesis of Osteoarthritis , 2014, Mediators of inflammation.

[38]  N. Willits,et al.  Synovial fluid growth factor and cytokine concentrations after intra-articular injection of a platelet-rich product in horses. , 2013, Veterinary journal.

[39]  R. Silva,et al.  Comparison of the effect of calcium gluconate and batroxobin on the release of transforming growth factor beta 1 in canine platelet concentrates , 2012, BMC Veterinary Research.

[40]  T. Albrektsson,et al.  Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF). , 2009, Trends in biotechnology.

[41]  W. E. van den Brom,et al.  Comparison of the effects of firocoxib, carprofen and vedaprofen in a sodium urate crystal induced synovitis model of arthritis in dogs. , 2008, Research in veterinary science.

[42]  P. Hanson,et al.  Clinical evaluation of firocoxib and carprofen for the treatment of dogs with osteoarthritis , 2006, Veterinary Record.

[43]  J. Wiltfang,et al.  Sample preparation technique and white cell content influence the detectable levels of growth factors in platelet concentrates , 2003, Vox sanguinis.

[44]  A. Manning,et al.  Cloning of a canine cDNA homologous to the human transforming growth factor-beta 1-encoding gene. , 1995, Gene.

[45]  R. LaPrade,et al.  The Influence of Naproxen on Biological Factors in Leukocyte-Rich Platelet-Rich Plasma: A Prospective Comparative Study. , 2019, Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association.

[46]  R. Marx,et al.  Platelet-rich plasma (PRP): what is PRP and what is not PRP? , 2001, Implant dentistry.