论文信息 - Electrochemical sensing of DNA immobilization and hybridization based on carbon nanotubes/nano zinc oxide/chitosan composite film

Electrochemical sensing of DNA immobilization and hybridization based on carbon nanotubes/nano zinc oxide/chitosan composite film

Abstract A novel electrochemical DNA biosensor based on zinc oxide (ZnO) nanoparticles and multi-walled carbon nanotubes (MWNTs) for DNA immobilization and enhanced hybridization detection is presented. The MWNTs/nano ZnO/chitosan composite film modified glassy carbon electrode (MWNTs/ZnO/CHIT/GCE) was fabricated and DNA probes were immobilized on the electrode surface. The hybridization events were monitored by differential pulse voltammetry (DPV) using methylene blue (MB) as an indicator. The sensor can effectively discriminate different DNA sequences related to PAT gene in the transgenic corn, with a detection limit of 2.8 × 10 −12 mol/L of target sequence.

[1] V. Zucolotto,et al. Physicochemical properties and sensing ability of metallophthalocyanines/chitosan nanocomposites. , 2006, The journal of physical chemistry. B.

[2] Guo-Li Shen,et al. A mediator-free phenol biosensor based on immobilizing tyrosinase to ZnO nanoparticles. , 2006, Analytical biochemistry.

[3] P. He,et al. Carbon nanotube-enhanced electrochemical DNA biosensor for DNA hybridization detection , 2003, Analytical and bioanalytical chemistry.

[4] J. Justin Gooding,et al. Evidence for the direct interaction between methylene blue and guanine bases using DNA-modified carbon paste electrodes , 2002 .

[5] Zhu Chang,et al. Electrochemically fabricated polyaniline nanowire-modified electrode for voltammetric detection of DNA hybridization , 2006 .

[6] S. Iijima. Helical microtubules of graphitic carbon , 1991, Nature.

[7] P. He,et al. Electrochemical DNA biosensors based on platinum nanoparticles combined carbon nanotubes , 2005 .