1H magnetic resonance spectroscopy of preinvasive and invasive cervical cancer: In vivo–ex vivo profiles and effect of tumor load

To compare in vivo 1H magnetic resonance (MR) spectra of preinvasive and invasive cervical lesions with ex vivo magic angle spinning (MAS) spectra of intact biopsies from the same subjects and to establish the effects of tumor load in the tissue sampled on the findings.

[1]  G. Sutherland,et al.  Mobile lipids and metabolic heterogeneity of brain tumours as detectable by Ex Vivo 1H MR spectroscopy , 1994, NMR in biomedicine.

[2]  D. Cory,et al.  Classification of human liposarcoma and lipoma using ex vivo proton NMR spectroscopy , 1999, Magnetic resonance in medicine.

[3]  W. Mackinnon,et al.  Uterine cervical punch biopsy specimens can be analyzed by 1h mrs , 1990, Magnetic resonance in medicine.

[4]  I. Cox,et al.  1H magnetic resonance spectroscopy of invasive cervical cancer: an in vivo study with ex vivo corroboration , 2004, NMR in biomedicine.

[5]  J. H. Lee,et al.  Localized in vivo 1H nuclear MR spectroscopy for evaluation of human uterine cervical carcinoma. , 1998, AJR. American journal of roentgenology.

[6]  D G Cory,et al.  Gradient, high‐resolution, magic angle spinning 1H nuclear magnetic resonance spectroscopy of intact cells , 1998, Magnetic resonance in medicine.

[7]  J. Griffin,et al.  The biochemical profile of rat testicular tissue as measured by magic angle spinning 1H NMR spectroscopy , 2000, FEBS letters.

[8]  N. deSouza,et al.  High-resolution MR imaging of stage I cervical neoplasia with a dedicated transvaginal coil: MR features and correlation of imaging and pathologic findings. , 1996, AJR. American journal of roentgenology.

[9]  Ian C. P. Smith,et al.  1H MRS of high grade astrocytomas: Mobile lipid accumulation in necrotic tissue , 1994, NMR in biomedicine.

[10]  H. Shimizu,et al.  Correlation between choline level measured by proton MR spectroscopy and Ki-67 labeling index in gliomas. , 2000, AJNR. American journal of neuroradiology.

[11]  J. Nelson,et al.  Detection of increased choline compounds with proton nuclear magnetic resonance spectroscopy subsequent to malignant transformation of human prostatic epithelial cells. , 2001, Cancer research.

[12]  K. Brindle,et al.  The appearance of neutral lipid signals in the 1H NMR spectra of a myeloma cell line correlates with the induced formation of cytoplasmic lipid droplets , 1993, Magnetic resonance in medicine.

[13]  F. Blankenberg,et al.  Quantitative analysis of apoptotic cell death using proton nuclear magnetic resonance spectroscopy. , 1997, Blood.

[14]  P. Flemming,et al.  MMP-1 and MMP-2 in the cervix uteri in different steps of malignant transformation--an immunohistochemical study. , 2002, Gynecologic oncology.

[15]  K. Brindle,et al.  Induction of apoptosis in two mammalian cell lines results in increased levels of fructose‐1,6‐Bisphosphate and CDP‐choline as determined by 31P MRS , 1998, Magnetic resonance in medicine.

[16]  C. Mountford,et al.  Organization of lipids in the plasma membranes of malignant and stimulated cells: a new model. , 1988, Trends in biochemical sciences.

[17]  M. Leach,et al.  The phosphocholine and glycerophosphocholine content of an oestrogen-sensitive rat mammary tumour correlates strongly with growth rate. , 1991, British Journal of Cancer.

[18]  R. Dina,et al.  A morphometric analysis of cytological features of tall cell variant and classical papillary carcinoma of the thyroid , 2000, Cytopathology : official journal of the British Society for Clinical Cytology.

[19]  A. Molinari,et al.  Lower levels of 1H MRS-visible mobile lipids in H-ras transformed tumorigenic fibroblasts with respect to their untransformed parental cells. , 1997, Cellular and molecular biology.

[20]  James R. Allen,et al.  In Vivo Proton (H1) Magnetic Resonance Spectroscopy for Cervical Carcinoma , 2001, American journal of clinical oncology.

[21]  J. Hu,et al.  High‐resolution 1H NMR spectroscopy in organs and tissues using slow magic angle spinning , 2001 .

[22]  M. Hoehn,et al.  1H‐ and 31P‐MR spectroscopy of primary and recurrent human brain tumors in vitro: malignancy‐characteristic profiles of water soluble and lipophilic spectral components , 2001, NMR in biomedicine.

[23]  S F Keevil,et al.  The performance of volume selection sequences for in vivo NMR spectroscopy: implications for quantitative MRS. , 2001, Magnetic resonance imaging.

[24]  Leo L. Cheng,et al.  Non‐destructive quantitation of spermine in human prostate tissue samples using HRMAS 1H NMR spectroscopy at 9.4 T , 2001, FEBS letters.

[25]  M J Bissell,et al.  Cellular changes involved in conversion of normal to malignant breast: importance of the stromal reaction. , 1996, Physiological reviews.

[26]  D J Collins,et al.  Quantification of signal selection efficiency, extra volume suppression and contamination for ISIS, STEAM and PRESS localized 1H NMR spectroscopy using an EEC localization test object , 1995, Physics in medicine and biology.

[27]  I. Gribbestad,et al.  1H NMR spectroscopic characterization of perchloric acid extracts from breast carcinomas and non‐involved breast tissue , 1994, NMR in biomedicine.

[28]  R. Sauter,et al.  Intratumoral lipids in 1H MRS in vivo in brain tumors: experience of the Siemens cooperative clinical trial. , 1996, Anticancer research.

[29]  C. Mountford,et al.  Proton MR and human cervical neoplasia: ex vivo spectroscopy allows distinction of invasive carcinoma of the cervix from carcinoma in situ and other preinvasive lesions. , 1993, Radiology.

[30]  L. Le Moyec,et al.  Proton nuclear magnetic resonance spectroscopy reveals cellular lipids involved in resistance to adriamycin and taxol by the K562 leukemia cell line. , 1996, Cancer research.

[31]  I Barba,et al.  The relationship between nuclear magnetic resonance-visible lipids, lipid droplets, and cell proliferation in cultured C6 cells. , 1999, Cancer research.

[32]  D. Rowley What Might A Stromal Response Mean to Prostate Cancer Progression? , 1998, Cancer and Metastasis Reviews.

[33]  J. Kurhanewicz,et al.  Citrate alterations in primary and metastatic human prostatic adenocarcinomas: 1H magnetic resonance spectroscopy and biochemical study , 1993, Magnetic resonance in medicine.

[34]  R. Gonzalez,et al.  Correlation of high-resolution magic angle spinning proton magnetic resonance spectroscopy with histopathology of intact human brain tumor specimens. , 1998, Cancer research.

[35]  S S Cross,et al.  Observer accuracy in estimating proportions in images: implications for the semiquantitative assessment of staining reactions and a proposal for a new system , 2001, Journal of clinical pathology.

[36]  A. Stringaro,et al.  Biophysical and structural characterization of 1H-NMR-detectable mobile lipid domains in NIH-3T3 fibroblasts. , 1999, Biochimica et biophysica acta.