Solution structure and dynamics of the DNA-binding domain of the adipocyte-transcription factor FREAC-11.

Transcription factors of the forkhead type share a highly conserved DNA-binding domain of about 100 amino acid residues. FREAC-11, expressed in adipocytes, belongs to this class. Here, we report on NMR studies that established the three-dimensional structure of the FREAC-11, DNA-binding domain. Although apparent similarities to the structures of other members within the forkhead family are observed, the structure also reveals some remarkable differences. Along with the complementary dynamics, the data provide insight into the fundamentals of sequence specificity within a highly conserved motif.

[1]  X. Liao,et al.  Dynamic DNA contacts observed in the NMR structure of winged helix protein-DNA complex. , 1999, Journal of molecular biology.

[2]  X. Liao,et al.  The dissociation rate of a winged helix protein-DNA complex is influenced by non-DNA contact residues. , 1999, Archives of biochemistry and biophysics.

[3]  L. Kay,et al.  Determination of the Protein Backbone Dihedral Angle ψ from a Combination of NMR-Derived Cross-Correlation Spin Relaxation Rates , 1998 .

[4]  X. Liao,et al.  Structural changes in the region directly adjacent to the DNA-binding helix highlight a possible mechanism to explain the observed changes in the sequence-specific binding of winged helix proteins. , 1998, Journal of molecular biology.

[5]  X. Liao,et al.  Sequence specific collective motions in a winged helix DNA binding domain detected by 15N relaxation NMR. , 1998, Biochemistry.

[6]  A. Gronenborn,et al.  The solution structure of a fungal AREA protein-DNA complex: an alternative binding mode for the basic carboxyl tail of GATA factors. , 1998, Journal of molecular biology.

[7]  L. Kay,et al.  A Sensitive Pulse Scheme for Measuring the Backbone Dihedral Angle ψ Based on Cross-correlation Between 13Cα-1Hα Dipolar and Carbonyl Chemical Shift Anisotropy Relaxation Interactions , 1998, Journal of biomolecular NMR.

[8]  X. Liao,et al.  Evidence that the DNA binding specificity of winged helix proteins is mediated by a structural change in the amino acid sequence adjacent to the principal DNA binding helix. , 1997, Biochemistry.

[9]  A. Bax,et al.  Determination of φ and χ1 Angles in Proteins from 13C−13C Three-Bond J Couplings Measured by Three-Dimensional Heteronuclear NMR. How Planar Is the Peptide Bond? , 1997 .

[10]  X. Liao,et al.  Different DNA contact schemes are used by two winged helix proteins to recognize a DNA binding sequence. , 1997, Nucleic acids research.

[11]  L. Kay,et al.  Pulse schemes for the measurement of3 JC′Cγ and3 JNCγ scalar couplings in 15N,13C uniformly labeled proteins , 1997 .

[12]  A. Bax,et al.  Anisotropic rotational diffusion of perdeuterated HIV protease from 15N NMR relaxation measurements at two magnetic fields , 1996, Journal of biomolecular NMR.

[13]  M. Klemsz,et al.  Genesis, a Winged Helix Transcriptional Repressor with Expression Restricted to Embryonic Stem Cells* , 1996, The Journal of Biological Chemistry.

[14]  W. Knöchel,et al.  Five years on the wings of fork head , 1996, Mechanisms of Development.

[15]  A. Bax,et al.  Rotational diffusion anisotropy of human ubiquitin from 15N NMR relaxation , 1995 .

[16]  S. Grzesiek,et al.  NMRPipe: A multidimensional spectral processing system based on UNIX pipes , 1995, Journal of biomolecular NMR.

[17]  M. Akke,et al.  Solution structure of (Cd2+)1-calbindin D9k reveals details of the stepwise structural changes along the Apo-->(Ca2+)II1-->(Ca2+)I,II2 binding pathway. , 1995, Journal of molecular biology.

[18]  A. Palmer,et al.  Backbone dynamics of Escherichia coli ribonuclease HI: correlations with structure and function in an active enzyme. , 1995, Journal of molecular biology.

[19]  J. Michael Schurr,et al.  A test of the model-free formulas. Effects of anisotropic rotational diffusion and dimerization. , 1994, Journal of magnetic resonance. Series B.

[20]  P. Carlsson,et al.  Cloning and characterization of seven human forkhead proteins: binding site specificity and DNA bending. , 1994, The EMBO journal.

[21]  T. Pawson,et al.  Backbone dynamics of a free and phosphopeptide-complexed Src homology 2 domain studied by 15N NMR relaxation. , 1994, Biochemistry.

[22]  R. Costa,et al.  The DNA-binding specificity of the hepatocyte nuclear factor 3/forkhead domain is influenced by amino-acid residues adjacent to the recognition helix , 1994, Molecular and cellular biology.

[23]  P. Kraulis,et al.  Solution structure and dynamics of ras p21.GDP determined by heteronuclear three- and four-dimensional NMR spectroscopy. , 1994, Biochemistry.

[24]  S. Burley,et al.  Co-crystal structure of the HNF-3/fork head DNA-recognition motif resembles histone H5 , 1993, Nature.

[25]  S. Enerbäck,et al.  Characterization of the human lipoprotein lipase (LPL) promoter: evidence of two cis-regulatory regions, LP-alpha and LP-beta, of importance for the differentiation-linked induction of the LPL gene during adipogenesis , 1992, Molecular and cellular biology.

[26]  P. Wright,et al.  Intramolecular motions of a zinc finger DNA-binding domain from Xfin characterized by proton-detected natural abundance carbon-13 heteronuclear NMR spectroscopy , 1991 .

[27]  H. Jäckle,et al.  The homeotic gene fork head encodes a nuclear protein and is expressed in the terminal regions of the Drosophila embryo , 1989, Cell.

[28]  J E Darnell,et al.  Multiple hepatocyte-enriched nuclear factors function in the regulation of transthyretin and alpha 1-antitrypsin genes , 1989, Molecular and cellular biology.

[29]  A. Szabó,et al.  Model-free approach to the interpretation of nuclear magnetic resonance relaxation in macromolecules. 1. Theory and range of validity , 1982 .

[30]  A. Szabó,et al.  Model-free approach to the interpretation of nuclear magnetic resonance relaxation in macromolecules. 2. Analysis of experimental results , 1982 .