Association between Dietary Habits and Fecal Microbiota Composition in Irritable Bowel Syndrome Patients: A Pilot Study

Intestinal dysbiosis seems to play a role in the pathophysiology of irritable bowel syndrome (IBS). The present pilot study aimed to elucidate the association between nutrient intake and Mediterranean diet (MD) adherence with IBS symptoms and gut microbiota in IBS patients. The nutrient intake of 28 IBS patients and 21 controls was assessed through a food diary, the reference intake ranges (RIs) for energy-yielding macronutrients and the MD serving score (MDSS) index. MD adherence and nutrients intake were compared to IBS symptoms and fecal microbiota, obtained by 16S rRNA targeted-metagenomics. In IBS patients MDSS index was altered compared to controls (p < 0.01). IBS patients with low-MD score reported severe abdominal pain and higher flatulence point-scales. Through Linear discriminant analysis effect size (LEfSe), Erysipelotrichaceae were detected as a microbial biomarker in IBS patients with altered RIs for macronutrients intake, compared to controls. Lactobacillaceae and Lactobacillus were associated to an altered carbohydrates intake in IBS patients, while specific taxonomic biomarkers, such as Aldercreuzia, Mogibacteriaceae, Rikenellaceae, Parabacteroides and F. prausnitzii were associated with an adequate intake of nutrient in these patients. This study supports an association between dietary patterns and gut microbial biomarkers in IBS patients. Further investigations are needed to clarify these connections.

[1]  D. Engelthaler,et al.  Isolation of Anti-Inflammatory and Epithelium Reinforcing Bacteroides and Parabacteroides Spp. from A Healthy Fecal Donor , 2020, Nutrients.

[2]  K. Fraser,et al.  Gut Microbial Metabolites and Biochemical Pathways Involved in Irritable Bowel Syndrome: Effects of Diet and Nutrition on the Microbiome , 2019, The Journal of nutrition.

[3]  D. Jonkers,et al.  Stool Consistency: Looking Beyond the Bristol Stool Form Scale , 2019, Journal of neurogastroenterology and motility.

[4]  M. Ciccozzi,et al.  Fecal and Mucosal Microbiota Profiling in Irritable Bowel Syndrome and Inflammatory Bowel Disease , 2019, Front. Microbiol..

[5]  S. Parthasarathy,et al.  Negative Effects of a High-Fat Diet on Intestinal Permeability: A Review. , 2019, Advances in nutrition.

[6]  P. Usai,et al.  Effect of Three Diets (Low-FODMAP, Gluten-free and Balanced) on Irritable Bowel Syndrome Symptoms and Health-Related Quality of Life , 2019, Nutrients.

[7]  Daniel S. Hitchcock,et al.  Bacteroides-Derived Sphingolipids Are Critical for Maintaining Intestinal Homeostasis and Symbiosis. , 2019, Cell host & microbe.

[8]  M. Ciccozzi,et al.  Gut mucosal-associated microbiota better discloses inflammatory bowel disease differential patterns than faecal microbiota. , 2019, Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver.

[9]  H. Zhang,et al.  Novel strains of Bacteroides fragilis and Bacteroides ovatus alleviate the LPS-induced inflammation in mice , 2019, Applied Microbiology and Biotechnology.

[10]  Inge A Lindseth,et al.  The Western Diet–Microbiome-Host Interaction and Its Role in Metabolic Disease , 2018, Nutrients.

[11]  D. Pardoll,et al.  Bacteroides fragilis Toxin Coordinates a Pro-carcinogenic Inflammatory Cascade via Targeting of Colonic Epithelial Cells. , 2018, Cell host & microbe.

[12]  B. Lacy,et al.  Rome Criteria and a Diagnostic Approach to Irritable Bowel Syndrome , 2017, Journal of clinical medicine.

[13]  S. S. Andrade,et al.  Action and function of Faecalibacterium prausnitzii in health and disease. , 2017, Best practice & research. Clinical gastroenterology.

[14]  P. Kashyap,et al.  Irritable bowel syndrome: a gut microbiota-related disorder? , 2017, American journal of physiology. Gastrointestinal and liver physiology.

[15]  W. Chey,et al.  A Randomized Controlled Trial Comparing the Low FODMAP Diet vs. Modified NICE Guidelines in US Adults with IBS-D , 2016, The American Journal of Gastroenterology.

[16]  Y. Benno,et al.  Comprehensive analysis of the fecal microbiota of healthy Japanese adults reveals a new bacterial lineage associated with a phenotype characterized by a high frequency of bowel movements and a lean body type , 2016, BMC Microbiology.

[17]  R. Cuomo,et al.  Good adherence to mediterranean diet can prevent gastrointestinal symptoms: A survey from Southern Italy , 2016, World journal of gastrointestinal pharmacology and therapeutics.

[18]  D. Drossman,et al.  Rome IV-Functional GI Disorders: Disorders of Gut-Brain Interaction. , 2016, Gastroenterology.

[19]  T. Preston,et al.  Formation of short chain fatty acids by the gut microbiota and their impact on human metabolism , 2016, Gut microbes.

[20]  K. Whelan,et al.  Altered gastrointestinal microbiota in irritable bowel syndrome and its modification by diet: probiotics, prebiotics and the low FODMAP diet , 2016, Proceedings of the Nutrition Society.

[21]  H. Flint,et al.  Changes in the Abundance of Faecalibacterium prausnitzii Phylogroups I and II in the Intestinal Mucosa of Inflammatory Bowel Disease and Patients with Colorectal Cancer , 2016, Inflammatory bowel diseases.

[22]  R. Spiller,et al.  Bowel Disorders. , 2016, Gastroenterology.

[23]  Y. Cong,et al.  Microbiota metabolite short chain fatty acids, GPCR, and inflammatory bowel diseases , 2016, Journal of Gastroenterology.

[24]  L. Böhn,et al.  Diet low in FODMAPs reduces symptoms of irritable bowel syndrome as well as traditional dietary advice: a randomized controlled trial. , 2015, Gastroenterology.

[25]  J. Štšepetova,et al.  New insights into the impact of Lactobacillus population on host-bacteria metabolic interplay , 2015, Oncotarget.

[26]  F. Olea-Serrano,et al.  Proposal of a Mediterranean Diet Serving Score , 2015, PloS one.

[27]  J. Steiner,et al.  The Fecal Microbiome in Cats with Diarrhea , 2015, PloS one.

[28]  A. Kane,et al.  Intestinal microbiota, microbial translocation, and systemic inflammation in chronic HIV infection. , 2015, The Journal of infectious diseases.

[29]  R. Soares.  Irritable bowel syndrome: a clinical review. , 2014, World journal of gastroenterology.

[30]  M. Bellini,et al.  Irritable bowel syndrome: a disease still searching for pathogenesis, diagnosis and therapy. , 2014, World journal of gastroenterology.

[31]  P. Gibson,et al.  Diets that differ in their FODMAP content alter the colonic luminal microenvironment , 2014, Gut.

[32]  Lawrence A. David,et al.  Diet rapidly and reproducibly alters the human gut microbiome , 2013, Nature.

[33]  M. J. Ramos-Peralonso.  European Food Safety Authority (EFSA) , 2014 .

[34]  C. Manichanh,et al.  Colonisation by Faecalibacterium prausnitzii and maintenance of clinical remission in patients with ulcerative colitis , 2013, Alimentary pharmacology & therapeutics.

[35]  C. Manichanh,et al.  Anal gas evacuation and colonic microbiota in patients with flatulence: effect of diet , 2013, Gut.

[36]  L. Böhn,et al.  Self-Reported Food-Related Gastrointestinal Symptoms in IBS Are Common and Associated With More Severe Symptoms and Reduced Quality of Life , 2013, The American Journal of Gastroenterology.

[37]  S. Mazmanian,et al.  Outer membrane vesicles of a human commensal mediate immune regulation and disease protection. , 2012, Cell host & microbe.

[38]  K. Harnett,et al.  Platelet‐activating factor and distinct chemokines are elevated in mucosal biopsies of erosive compared with non‐erosive reflux disease patients and controls , 2012, Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society.

[39]  C. Loguercio,et al.  Focus on irritable bowel syndrome. , 2012, European review for medical and pharmacological sciences.

[40]  Michele Iacono,et al.  “Remake” by High-Throughput Sequencing of the Microbiota Involved in the Production of Water Buffalo Mozzarella Cheese , 2012, Applied and Environmental Microbiology.

[41]  K. Whelan,et al.  Fermentable carbohydrate restriction reduces luminal bifidobacteria and gastrointestinal symptoms in patients with irritable bowel syndrome. , 2012, The Journal of nutrition.

[42]  Jennifer M. Brulc,et al.  454 pyrosequencing reveals a shift in fecal microbiota of healthy adult men consuming polydextrose or soluble corn fiber. , 2012, The Journal of nutrition.

[43]  T. Hausken,et al.  Diet and effects of diet management on quality of life and symptoms in patients with irritable bowel syndrome. , 2012, Molecular medicine reports.

[44]  T. Hausken,et al.  The role of diet in the pathogenesis and management of irritable bowel syndrome (Review). , 2012, International journal of molecular medicine.

[45]  R. Alfenas,et al.  Influence of a high-fat diet on gut microbiota, intestinal permeability and metabolic endotoxaemia , 2012, British Journal of Nutrition.

[46]  K. Whelan,et al.  Distinct microbial populations exist in the mucosa‐associated microbiota of sub‐groups of irritable bowel syndrome , 2012, Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society.

[47]  Marcus J Claesson,et al.  An irritable bowel syndrome subtype defined by species-specific alterations in faecal microbiota , 2011, Gut.

[48]  A. Trichopoulou,et al.  Mediterranean diet pyramid today. Science and cultural updates , 2011, Public Health Nutrition.

[49]  K. Whelan,et al.  Comparison of symptom response following advice for a diet low in fermentable carbohydrates (FODMAPs) versus standard dietary advice in patients with irritable bowel syndrome. , 2011, Journal of human nutrition and dietetics : the official journal of the British Dietetic Association.

[50]  C. Huttenhower,et al.  Metagenomic biomarker discovery and explanation , 2011, Genome Biology.

[51]  C. Manichanh,et al.  Colonization by Faecalibacterium Prausnitzii and Maintenance of Clinical Remission in Patients With Ulcerative Colitis , 2011 .

[52]  B. Corfe,et al.  Dietary intakes in people with irritable bowel syndrome , 2011, BMC gastroenterology.

[53]  R. Sartor,et al.  Luminal and mucosal-associated intestinal microbiota in patients with diarrhea-predominant irritable bowel syndrome , 2010, Gut pathogens.

[54]  Robert C. Edgar,et al.  Search and clustering orders of magnitude faster than BLAST , 2010, Bioinform..

[55]  R. Knight,et al.  Rapid denoising of pyrosequencing amplicon data: exploiting the rank-abundance distribution , 2010, Nature Methods.

[56]  S. Morini,et al.  Effect of ursodeoxycholic acid on inflammatory infiltrate in gallbladder muscle of cholesterol gallstone patients , 2010, Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society.

[57]  William A. Walters,et al.  QIIME allows analysis of high-throughput community sequencing data , 2010, Nature Methods.

[58]  E. Dusseldorp,et al.  Testing a biobehavioral model of irritable bowel syndrome , 2010, European journal of gastroenterology & hepatology.

[59]  P. Gibson,et al.  Comparison of the prevalence of fructose and lactose malabsorption across chronic intestinal disorders , 2009, Alimentary pharmacology & therapeutics.

[60]  M. Samsom,et al.  Lower Bifidobacteria counts in both duodenal mucosa-associated and fecal microbiota in irritable bowel syndrome patients. , 2009, World journal of gastroenterology.

[61]  A. Benson,et al.  Diet-Induced Metabolic Improvements in a Hamster Model of Hypercholesterolemia Are Strongly Linked to Alterations of the Gut Microbiota , 2009, Applied and Environmental Microbiology.

[62]  M. Crowell,et al.  Human gut microbiota in obesity and after gastric bypass , 2009, Proceedings of the National Academy of Sciences.

[63]  J. Doré,et al.  Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients , 2008, Proceedings of the National Academy of Sciences.

[64]  G. Holtrop,et al.  Effect of inulin on the human gut microbiota: stimulation of Bifidobacterium adolescentis and Faecalibacterium prausnitzii , 2008, British Journal of Nutrition.

[65]  Lin Chang,et al.  FUNCTIONAL BOWEL DISORDERS , 2018, The American Journal of Gastroenterology.

[66]  S. Miehlke,et al.  Prevalence of Bacteroides and Prevotella spp. in ulcerative colitis. , 2006, Journal of medical microbiology.

[67]  H. Ingmer,et al.  Proteolytic systems of lactic acid bacteria , 2006, Applied Microbiology and Biotechnology.

[68]  C. Cherbut,et al.  Lactate is mainly fermented to butyrate by human intestinal microfloras but inter‐individual variation is evident , 2005, Journal of applied microbiology.

[69]  A. Zinsmeister,et al.  Diet and Functional Gastrointestinal Disorders: A Population-Based Case—Control Study , 2003, The American Journal of Gastroenterology.

[70]  S. Murphy.  FOOD COMPOSITION DATA , 2005 .

[71]  A. Elfering,et al.  Pain assessment , 2005, European Spine Journal.

[72]  A. Zinsmeister,et al.  Diet and functional gastrointestinal disorders: a population based case-control study , 2003, American Journal of Gastroenterology.

[73]  M. Fons,et al.  Ruminococcin A, a New Lantibiotic Produced by aRuminococcus gnavus Strain Isolated from Human Feces , 2001, Applied and Environmental Microbiology.

[74]  Ulf Bengtsson,et al.  Food-Related Gastrointestinal Symptoms in the Irritable Bowel Syndrome , 2001, Digestion.

[75]  S. Lewis,et al.  Stool form scale as a useful guide to intestinal transit time. , 1997, Scandinavian journal of gastroenterology.

[76]  J. Cummings,et al.  Does digestibility of meat protein help explain large bowel cancer risk? , 1995, Nutrition and cancer.

[77]  M. Heitkemper,et al.  Comparison of diet composition in women with and without functional bowel disorder. , 1994, Gastroenterology nursing : the official journal of the Society of Gastroenterology Nurses and Associates.

[78]  F. Fidanza,et al.  Tabelle di composizione degli alimenti , 1987 .