A new hybrid signal amplification strategy for ultrasensitive electrochemical detection of DNA based on enzyme-assisted target recycling and DNA supersandwich assemblies.

A highly sensitive electrochemical sequence-specific DNA detection strategy is demonstrated by coupling N.BstNB I (a nicking endonuclease)-assisted target recycling amplification with DNA supersandwich assembly signal enhancement. The proposed method avoids any extra chemical labeling steps and offers high selectivity against single-base mismatch sequences and a low detection limit down to 0.36 fM.

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