Nutritional and Environmental Factors Affecting Cellulase Production by Two Strains of Cellulolytic Bacilli

Effect of some nutritional and environmental factors on g ro wt h and cellulase production 3 by Bacillus alcalophilus S39 and Bacillus amyloliquefaciens C2 was inves tigated. Results indicated t h a t 1% carboxymethylcellulose (CMC) and 0.7% yeas t extract were mos t effective as the carbon an d nitro g e n s o urces respectively. Initial pH 7 and 3% inoculum s ize found to be optimal for growth and cellu la se production. Incubation temperature at 30 and 45oC achieved the highes t activity of cellulase 3 for Bacillus alcalophilus S39 and Bacillus amyloliquefaciens C2 respectively, and the suitable shaking rate was 150 and 200 rpm.

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