Direct immunosensing using differential electrochemical measurements of impedimetric variations

In this paper a new electrochemical method for direct immunodetection in real time is presented. The method is based on measurement of the impedimetric variations due to interaction with antigens on platinum electrodes functionalized with polymeric layers containing antibodies. In contrast to a classical impedimetric method, our method allows a differential mode of measurement, ensuring the specificity of the signal. The set-up includes a flow injection system, which is a convenient method of changing solutions and displaying the effect of the different parameters on the resulting responses.

[1]  Michael Grätzel,et al.  Detection of viruses and bacteria with piezoelectric immunosensors , 1993 .

[2]  K. A. Davis,et al.  Continuous liquid-phase piezoelectric biosensor for kinetic immunoassays. , 1989, Analytical chemistry.

[3]  M. Maeda,et al.  Electrode Modification by Long-Chain, Dialkyl Disulfide Reagent Haying Terminal Dinitrophenyl Group and Its Application to Impedimetric Immunosensors , 1993 .

[4]  J. Therasse,et al.  Toxin detection using capacitance measurements on immunospecies grafted onto a semiconductor substrate , 1991 .

[5]  R. Murray,et al.  Chemically modified electrodes: XV. Electrochemistry and waveshape analysis of aminophenylferrocene bonded to acid chloride functionalized ruthenium, platinum, and tin oxide electrodes , 1979 .

[6]  N. Jaffrezic‐Renault,et al.  Impedance analysis of Si/SiO2 heterostructures grafted with antibodies: an approach for immunosensor development , 1996 .

[7]  M. J. Eddowes,et al.  Surface plasmon resonance applied to immunosensing , 1988 .

[8]  R. Murray,et al.  Chemically modified electrodes. 13. Monolayer/multilayer coverage, decay kinetics, and solvent and interaction effects for ferrocenes covalently linked to platinum electrodes , 1978 .

[9]  R. Murray,et al.  Chemically modified electrodes Part VII. Covalent bonding of a reversible electrode reactant to Pt electrodes using an organosilane reagent , 1977 .

[10]  M. Keddam.  Electrochimie, principes, methodes et applications : A.J. Bard et L. R. Faulkner Masson, Paris, 1983, 781 pp. , 1985 .