Stable H3K4me3 is associated with transcription initiation during early embryo development

MOTIVATION During development of the mammalian embryo, histone modification H3K4me3 plays an important role in regulating gene expression and exhibits extensive reprogramming on the parental genomes. In addition to these dramatic epigenetic changes, certain unchanging regulatory elements are also essential for embryonic development. RESULTS Using large-scale H3K4me3 chromatin immunoprecipitation sequencing (ChIP-Seq) data, we identified a form of H3K4me3 that was present during all 8 stages of the mouse embryo before implantation. This "stable H3K4me3" was highly accessible and much longer than normal H3K4me3. Moreover, most of the stable H3K4me3 was in the promoter region and was enriched in higher chromatin architecture. Using in-depth analysis, we demonstrated that stable H3K4me3 was related to higher gene expression levels and transcriptional initiation during embryonic development. Furthermore, stable H3K4me3 was much more active in blood tumor cells than in normal blood cells, suggesting a potential mechanism of cancer progression. SUPPLEMENTARY INFORMATION Supplementary data are available at Bioinformatics online.

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